Job ID = 2010516 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #14024705 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #16385 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #7012353 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #17530881 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035321' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #10518529 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T14:24:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:25:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:26:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:30:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:33:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:38:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:38:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 21,020,869 reads read : 42,041,738 reads written : 42,041,738 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:20 21020869 reads; of these: 21020869 (100.00%) were paired; of these: 6636812 (31.57%) aligned concordantly 0 times 10973563 (52.20%) aligned concordantly exactly 1 time 3410494 (16.22%) aligned concordantly >1 times ---- 6636812 pairs aligned concordantly 0 times; of these: 456498 (6.88%) aligned discordantly 1 time ---- 6180314 pairs aligned 0 times concordantly or discordantly; of these: 12360628 mates make up the pairs; of these: 11795388 (95.43%) aligned 0 times 227564 (1.84%) aligned exactly 1 time 337676 (2.73%) aligned >1 times 71.94% overall alignment rate Time searching: 00:14:20 Overall time: 00:14:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2656904 / 14826191 = 0.1792 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:08:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:08:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:08:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:08:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:08:32: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:08:32: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:08:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:08:33: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:08:33: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:08:38: 1000000 INFO @ Sat, 06 Jul 2019 00:08:40: 1000000 INFO @ Sat, 06 Jul 2019 00:08:41: 1000000 INFO @ Sat, 06 Jul 2019 00:08:45: 2000000 INFO @ Sat, 06 Jul 2019 00:08:48: 2000000 INFO @ Sat, 06 Jul 2019 00:08:49: 2000000 INFO @ Sat, 06 Jul 2019 00:08:53: 3000000 INFO @ Sat, 06 Jul 2019 00:08:56: 3000000 INFO @ Sat, 06 Jul 2019 00:08:57: 3000000 INFO @ Sat, 06 Jul 2019 00:09:00: 4000000 INFO @ Sat, 06 Jul 2019 00:09:05: 4000000 INFO @ Sat, 06 Jul 2019 00:09:06: 4000000 INFO @ Sat, 06 Jul 2019 00:09:07: 5000000 INFO @ Sat, 06 Jul 2019 00:09:13: 5000000 INFO @ Sat, 06 Jul 2019 00:09:14: 5000000 INFO @ Sat, 06 Jul 2019 00:09:14: 6000000 INFO @ Sat, 06 Jul 2019 00:09:21: 6000000 INFO @ Sat, 06 Jul 2019 00:09:21: 7000000 INFO @ Sat, 06 Jul 2019 00:09:22: 6000000 INFO @ Sat, 06 Jul 2019 00:09:29: 7000000 INFO @ Sat, 06 Jul 2019 00:09:29: 8000000 INFO @ Sat, 06 Jul 2019 00:09:30: 7000000 INFO @ Sat, 06 Jul 2019 00:09:37: 9000000 INFO @ Sat, 06 Jul 2019 00:09:38: 8000000 INFO @ Sat, 06 Jul 2019 00:09:39: 8000000 INFO @ Sat, 06 Jul 2019 00:09:44: 10000000 INFO @ Sat, 06 Jul 2019 00:09:46: 9000000 INFO @ Sat, 06 Jul 2019 00:09:47: 9000000 INFO @ Sat, 06 Jul 2019 00:09:52: 11000000 INFO @ Sat, 06 Jul 2019 00:09:54: 10000000 INFO @ Sat, 06 Jul 2019 00:09:55: 10000000 INFO @ Sat, 06 Jul 2019 00:09:59: 12000000 INFO @ Sat, 06 Jul 2019 00:10:02: 11000000 INFO @ Sat, 06 Jul 2019 00:10:03: 11000000 INFO @ Sat, 06 Jul 2019 00:10:06: 13000000 INFO @ Sat, 06 Jul 2019 00:10:11: 12000000 INFO @ Sat, 06 Jul 2019 00:10:12: 12000000 INFO @ Sat, 06 Jul 2019 00:10:14: 14000000 INFO @ Sat, 06 Jul 2019 00:10:19: 13000000 INFO @ Sat, 06 Jul 2019 00:10:20: 13000000 INFO @ Sat, 06 Jul 2019 00:10:21: 15000000 INFO @ Sat, 06 Jul 2019 00:10:27: 14000000 INFO @ Sat, 06 Jul 2019 00:10:28: 14000000 INFO @ Sat, 06 Jul 2019 00:10:28: 16000000 INFO @ Sat, 06 Jul 2019 00:10:35: 15000000 INFO @ Sat, 06 Jul 2019 00:10:35: 17000000 INFO @ Sat, 06 Jul 2019 00:10:36: 15000000 INFO @ Sat, 06 Jul 2019 00:10:42: 18000000 INFO @ Sat, 06 Jul 2019 00:10:43: 16000000 INFO @ Sat, 06 Jul 2019 00:10:44: 16000000 INFO @ Sat, 06 Jul 2019 00:10:49: 19000000 INFO @ Sat, 06 Jul 2019 00:10:51: 17000000 INFO @ Sat, 06 Jul 2019 00:10:52: 17000000 INFO @ Sat, 06 Jul 2019 00:10:56: 20000000 INFO @ Sat, 06 Jul 2019 00:10:59: 18000000 INFO @ Sat, 06 Jul 2019 00:11:00: 18000000 INFO @ Sat, 06 Jul 2019 00:11:03: 21000000 INFO @ Sat, 06 Jul 2019 00:11:07: 19000000 INFO @ Sat, 06 Jul 2019 00:11:08: 19000000 INFO @ Sat, 06 Jul 2019 00:11:10: 22000000 INFO @ Sat, 06 Jul 2019 00:11:15: 20000000 INFO @ Sat, 06 Jul 2019 00:11:16: 20000000 INFO @ Sat, 06 Jul 2019 00:11:17: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:11:23: 21000000 INFO @ Sat, 06 Jul 2019 00:11:24: 21000000 INFO @ Sat, 06 Jul 2019 00:11:25: 24000000 INFO @ Sat, 06 Jul 2019 00:11:30: 22000000 INFO @ Sat, 06 Jul 2019 00:11:31: 22000000 INFO @ Sat, 06 Jul 2019 00:11:32: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:11:32: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:11:32: #1 total tags in treatment: 11780600 INFO @ Sat, 06 Jul 2019 00:11:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:11:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:11:32: #1 tags after filtering in treatment: 7259755 INFO @ Sat, 06 Jul 2019 00:11:32: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 06 Jul 2019 00:11:32: #1 finished! INFO @ Sat, 06 Jul 2019 00:11:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:11:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:11:32: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:11:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:11:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:11:38: 23000000 INFO @ Sat, 06 Jul 2019 00:11:39: 23000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 00:11:46: 24000000 INFO @ Sat, 06 Jul 2019 00:11:47: 24000000 INFO @ Sat, 06 Jul 2019 00:11:53: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:11:53: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:11:53: #1 total tags in treatment: 11780600 INFO @ Sat, 06 Jul 2019 00:11:53: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:11:54: #1 tags after filtering in treatment: 7259755 INFO @ Sat, 06 Jul 2019 00:11:54: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 06 Jul 2019 00:11:54: #1 finished! INFO @ Sat, 06 Jul 2019 00:11:54: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:11:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:11:54: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:11:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:11:54: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:11:54: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:11:54: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:11:54: #1 total tags in treatment: 11780600 INFO @ Sat, 06 Jul 2019 00:11:54: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:11:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:11:55: #1 tags after filtering in treatment: 7259755 INFO @ Sat, 06 Jul 2019 00:11:55: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 06 Jul 2019 00:11:55: #1 finished! INFO @ Sat, 06 Jul 2019 00:11:55: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:11:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:11:55: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:11:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:11:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381220/SRX381220.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling