Job ID = 2010514 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #10698753 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #3571713 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #17825793 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035320' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #16385 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T14:23:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:23:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:30:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:32:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:38:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 21,385,281 reads read : 42,770,562 reads written : 42,770,562 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:59 21385281 reads; of these: 21385281 (100.00%) were paired; of these: 1478948 (6.92%) aligned concordantly 0 times 17569702 (82.16%) aligned concordantly exactly 1 time 2336631 (10.93%) aligned concordantly >1 times ---- 1478948 pairs aligned concordantly 0 times; of these: 275883 (18.65%) aligned discordantly 1 time ---- 1203065 pairs aligned 0 times concordantly or discordantly; of these: 2406130 mates make up the pairs; of these: 1971761 (81.95%) aligned 0 times 270107 (11.23%) aligned exactly 1 time 164262 (6.83%) aligned >1 times 95.39% overall alignment rate Time searching: 00:18:59 Overall time: 00:18:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1698760 / 20161514 = 0.0843 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:14:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:14:38: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:14:38: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:14:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:14:39: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:14:39: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:14:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:14:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:14:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:14:45: 1000000 INFO @ Sat, 06 Jul 2019 00:14:47: 1000000 INFO @ Sat, 06 Jul 2019 00:14:48: 1000000 INFO @ Sat, 06 Jul 2019 00:14:52: 2000000 INFO @ Sat, 06 Jul 2019 00:14:54: 2000000 INFO @ Sat, 06 Jul 2019 00:14:55: 2000000 INFO @ Sat, 06 Jul 2019 00:14:58: 3000000 INFO @ Sat, 06 Jul 2019 00:15:02: 3000000 INFO @ Sat, 06 Jul 2019 00:15:03: 3000000 INFO @ Sat, 06 Jul 2019 00:15:05: 4000000 INFO @ Sat, 06 Jul 2019 00:15:09: 4000000 INFO @ Sat, 06 Jul 2019 00:15:10: 4000000 INFO @ Sat, 06 Jul 2019 00:15:12: 5000000 INFO @ Sat, 06 Jul 2019 00:15:18: 6000000 INFO @ Sat, 06 Jul 2019 00:15:19: 5000000 INFO @ Sat, 06 Jul 2019 00:15:19: 5000000 INFO @ Sat, 06 Jul 2019 00:15:25: 7000000 INFO @ Sat, 06 Jul 2019 00:15:27: 6000000 INFO @ Sat, 06 Jul 2019 00:15:27: 6000000 INFO @ Sat, 06 Jul 2019 00:15:32: 8000000 INFO @ Sat, 06 Jul 2019 00:15:35: 7000000 INFO @ Sat, 06 Jul 2019 00:15:35: 7000000 INFO @ Sat, 06 Jul 2019 00:15:38: 9000000 INFO @ Sat, 06 Jul 2019 00:15:43: 8000000 INFO @ Sat, 06 Jul 2019 00:15:43: 8000000 INFO @ Sat, 06 Jul 2019 00:15:45: 10000000 INFO @ Sat, 06 Jul 2019 00:15:51: 9000000 INFO @ Sat, 06 Jul 2019 00:15:51: 9000000 INFO @ Sat, 06 Jul 2019 00:15:51: 11000000 INFO @ Sat, 06 Jul 2019 00:15:58: 12000000 INFO @ Sat, 06 Jul 2019 00:15:59: 10000000 INFO @ Sat, 06 Jul 2019 00:15:59: 10000000 INFO @ Sat, 06 Jul 2019 00:16:04: 13000000 INFO @ Sat, 06 Jul 2019 00:16:06: 11000000 INFO @ Sat, 06 Jul 2019 00:16:07: 11000000 INFO @ Sat, 06 Jul 2019 00:16:11: 14000000 INFO @ Sat, 06 Jul 2019 00:16:14: 12000000 INFO @ Sat, 06 Jul 2019 00:16:14: 12000000 INFO @ Sat, 06 Jul 2019 00:16:17: 15000000 INFO @ Sat, 06 Jul 2019 00:16:22: 13000000 INFO @ Sat, 06 Jul 2019 00:16:22: 13000000 INFO @ Sat, 06 Jul 2019 00:16:24: 16000000 INFO @ Sat, 06 Jul 2019 00:16:30: 14000000 INFO @ Sat, 06 Jul 2019 00:16:30: 14000000 INFO @ Sat, 06 Jul 2019 00:16:30: 17000000 INFO @ Sat, 06 Jul 2019 00:16:37: 15000000 INFO @ Sat, 06 Jul 2019 00:16:37: 18000000 INFO @ Sat, 06 Jul 2019 00:16:39: 15000000 INFO @ Sat, 06 Jul 2019 00:16:44: 19000000 INFO @ Sat, 06 Jul 2019 00:16:44: 16000000 INFO @ Sat, 06 Jul 2019 00:16:47: 16000000 INFO @ Sat, 06 Jul 2019 00:16:50: 20000000 INFO @ Sat, 06 Jul 2019 00:16:51: 17000000 INFO @ Sat, 06 Jul 2019 00:16:56: 17000000 INFO @ Sat, 06 Jul 2019 00:16:57: 21000000 INFO @ Sat, 06 Jul 2019 00:16:59: 18000000 INFO @ Sat, 06 Jul 2019 00:17:04: 22000000 INFO @ Sat, 06 Jul 2019 00:17:05: 18000000 INFO @ Sat, 06 Jul 2019 00:17:06: 19000000 INFO @ Sat, 06 Jul 2019 00:17:10: 23000000 INFO @ Sat, 06 Jul 2019 00:17:13: 19000000 INFO @ Sat, 06 Jul 2019 00:17:13: 20000000 INFO @ Sat, 06 Jul 2019 00:17:17: 24000000 INFO @ Sat, 06 Jul 2019 00:17:21: 21000000 INFO @ Sat, 06 Jul 2019 00:17:22: 20000000 INFO @ Sat, 06 Jul 2019 00:17:23: 25000000 INFO @ Sat, 06 Jul 2019 00:17:28: 22000000 INFO @ Sat, 06 Jul 2019 00:17:30: 26000000 INFO @ Sat, 06 Jul 2019 00:17:30: 21000000 INFO @ Sat, 06 Jul 2019 00:17:35: 23000000 INFO @ Sat, 06 Jul 2019 00:17:37: 27000000 INFO @ Sat, 06 Jul 2019 00:17:39: 22000000 INFO @ Sat, 06 Jul 2019 00:17:43: 24000000 INFO @ Sat, 06 Jul 2019 00:17:43: 28000000 INFO @ Sat, 06 Jul 2019 00:17:48: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:17:50: 29000000 INFO @ Sat, 06 Jul 2019 00:17:50: 25000000 INFO @ Sat, 06 Jul 2019 00:17:56: 24000000 INFO @ Sat, 06 Jul 2019 00:17:56: 30000000 INFO @ Sat, 06 Jul 2019 00:17:57: 26000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 00:18:03: 31000000 INFO @ Sat, 06 Jul 2019 00:18:05: 27000000 INFO @ Sat, 06 Jul 2019 00:18:05: 25000000 INFO @ Sat, 06 Jul 2019 00:18:10: 32000000 INFO @ Sat, 06 Jul 2019 00:18:12: 28000000 INFO @ Sat, 06 Jul 2019 00:18:14: 26000000 INFO @ Sat, 06 Jul 2019 00:18:16: 33000000 INFO @ Sat, 06 Jul 2019 00:18:19: 29000000 INFO @ Sat, 06 Jul 2019 00:18:23: 27000000 INFO @ Sat, 06 Jul 2019 00:18:23: 34000000 INFO @ Sat, 06 Jul 2019 00:18:27: 30000000 INFO @ Sat, 06 Jul 2019 00:18:30: 35000000 INFO @ Sat, 06 Jul 2019 00:18:32: 28000000 INFO @ Sat, 06 Jul 2019 00:18:34: 31000000 INFO @ Sat, 06 Jul 2019 00:18:36: 36000000 INFO @ Sat, 06 Jul 2019 00:18:40: 29000000 INFO @ Sat, 06 Jul 2019 00:18:41: 32000000 INFO @ Sat, 06 Jul 2019 00:18:43: 37000000 INFO @ Sat, 06 Jul 2019 00:18:46: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:18:46: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:18:46: #1 total tags in treatment: 18217453 INFO @ Sat, 06 Jul 2019 00:18:46: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:18:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:18:46: #1 tags after filtering in treatment: 5484037 INFO @ Sat, 06 Jul 2019 00:18:46: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 06 Jul 2019 00:18:46: #1 finished! INFO @ Sat, 06 Jul 2019 00:18:46: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:18:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:18:47: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:18:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:18:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:18:49: 33000000 INFO @ Sat, 06 Jul 2019 00:18:49: 30000000 INFO @ Sat, 06 Jul 2019 00:18:56: 34000000 INFO @ Sat, 06 Jul 2019 00:18:58: 31000000 INFO @ Sat, 06 Jul 2019 00:19:03: 35000000 INFO @ Sat, 06 Jul 2019 00:19:06: 32000000 INFO @ Sat, 06 Jul 2019 00:19:10: 36000000 INFO @ Sat, 06 Jul 2019 00:19:15: 33000000 INFO @ Sat, 06 Jul 2019 00:19:18: 37000000 INFO @ Sat, 06 Jul 2019 00:19:21: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:19:21: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:19:21: #1 total tags in treatment: 18217453 INFO @ Sat, 06 Jul 2019 00:19:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:19:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:19:21: #1 tags after filtering in treatment: 5484037 INFO @ Sat, 06 Jul 2019 00:19:21: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 06 Jul 2019 00:19:21: #1 finished! INFO @ Sat, 06 Jul 2019 00:19:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:19:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:19:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:19:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:19:22: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:19:23: 34000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:19:32: 35000000 INFO @ Sat, 06 Jul 2019 00:19:40: 36000000 INFO @ Sat, 06 Jul 2019 00:19:48: 37000000 INFO @ Sat, 06 Jul 2019 00:19:52: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:19:52: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:19:52: #1 total tags in treatment: 18217453 INFO @ Sat, 06 Jul 2019 00:19:52: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:19:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:19:53: #1 tags after filtering in treatment: 5484037 INFO @ Sat, 06 Jul 2019 00:19:53: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 06 Jul 2019 00:19:53: #1 finished! INFO @ Sat, 06 Jul 2019 00:19:53: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:19:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:19:53: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:19:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:19:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381219/SRX381219.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling