Job ID = 2010513 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:39 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #10387457 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #15564801 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T13:49:41 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: connection busy while validating within network system module - Failed to KHttpFileRead('https://sra-download.ncbi.nlm.nih.gov/traces/sra15/SRR/001011/SRR1035319' (), 32768) from '172.19.7.77' 2019-07-05T13:49:41 fasterq-dump.2.9.6 err: cmn_iter.c cmn_read_String( #20758529 ).VCursorCellDataDirect() -> RC(rcNS,rcNoTarg,rcValidating,rcConnection,rcBusy) 2019-07-05T14:24:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:27:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:27:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T14:53:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 31,118,805 reads read : 62,237,610 reads written : 62,237,610 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:11 31118805 reads; of these: 31118805 (100.00%) were paired; of these: 2667190 (8.57%) aligned concordantly 0 times 23541253 (75.65%) aligned concordantly exactly 1 time 4910362 (15.78%) aligned concordantly >1 times ---- 2667190 pairs aligned concordantly 0 times; of these: 1072969 (40.23%) aligned discordantly 1 time ---- 1594221 pairs aligned 0 times concordantly or discordantly; of these: 3188442 mates make up the pairs; of these: 2042008 (64.04%) aligned 0 times 575238 (18.04%) aligned exactly 1 time 571196 (17.91%) aligned >1 times 96.72% overall alignment rate Time searching: 00:25:11 Overall time: 00:25:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1596247 / 29276865 = 0.0545 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 00:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:47:12: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:47:12: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:47:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:47:13: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:47:13: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 00:47:14: #1 read tag files... INFO @ Sat, 06 Jul 2019 00:47:14: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 00:47:22: 1000000 INFO @ Sat, 06 Jul 2019 00:47:24: 1000000 INFO @ Sat, 06 Jul 2019 00:47:25: 1000000 INFO @ Sat, 06 Jul 2019 00:47:31: 2000000 INFO @ Sat, 06 Jul 2019 00:47:35: 2000000 INFO @ Sat, 06 Jul 2019 00:47:36: 2000000 INFO @ Sat, 06 Jul 2019 00:47:41: 3000000 INFO @ Sat, 06 Jul 2019 00:47:45: 3000000 INFO @ Sat, 06 Jul 2019 00:47:46: 3000000 INFO @ Sat, 06 Jul 2019 00:47:50: 4000000 INFO @ Sat, 06 Jul 2019 00:47:56: 4000000 INFO @ Sat, 06 Jul 2019 00:47:57: 4000000 INFO @ Sat, 06 Jul 2019 00:48:00: 5000000 INFO @ Sat, 06 Jul 2019 00:48:07: 5000000 INFO @ Sat, 06 Jul 2019 00:48:08: 5000000 INFO @ Sat, 06 Jul 2019 00:48:09: 6000000 INFO @ Sat, 06 Jul 2019 00:48:18: 6000000 INFO @ Sat, 06 Jul 2019 00:48:19: 7000000 INFO @ Sat, 06 Jul 2019 00:48:19: 6000000 INFO @ Sat, 06 Jul 2019 00:48:28: 8000000 INFO @ Sat, 06 Jul 2019 00:48:29: 7000000 INFO @ Sat, 06 Jul 2019 00:48:30: 7000000 INFO @ Sat, 06 Jul 2019 00:48:38: 9000000 INFO @ Sat, 06 Jul 2019 00:48:39: 8000000 INFO @ Sat, 06 Jul 2019 00:48:41: 8000000 INFO @ Sat, 06 Jul 2019 00:48:47: 10000000 INFO @ Sat, 06 Jul 2019 00:48:50: 9000000 INFO @ Sat, 06 Jul 2019 00:48:51: 9000000 INFO @ Sat, 06 Jul 2019 00:48:56: 11000000 INFO @ Sat, 06 Jul 2019 00:49:01: 10000000 INFO @ Sat, 06 Jul 2019 00:49:02: 10000000 INFO @ Sat, 06 Jul 2019 00:49:06: 12000000 INFO @ Sat, 06 Jul 2019 00:49:12: 11000000 INFO @ Sat, 06 Jul 2019 00:49:13: 11000000 INFO @ Sat, 06 Jul 2019 00:49:15: 13000000 INFO @ Sat, 06 Jul 2019 00:49:23: 12000000 INFO @ Sat, 06 Jul 2019 00:49:24: 12000000 INFO @ Sat, 06 Jul 2019 00:49:24: 14000000 INFO @ Sat, 06 Jul 2019 00:49:33: 13000000 INFO @ Sat, 06 Jul 2019 00:49:33: 15000000 INFO @ Sat, 06 Jul 2019 00:49:34: 13000000 INFO @ Sat, 06 Jul 2019 00:49:43: 16000000 INFO @ Sat, 06 Jul 2019 00:49:44: 14000000 INFO @ Sat, 06 Jul 2019 00:49:45: 14000000 INFO @ Sat, 06 Jul 2019 00:49:52: 17000000 INFO @ Sat, 06 Jul 2019 00:49:55: 15000000 INFO @ Sat, 06 Jul 2019 00:49:55: 15000000 INFO @ Sat, 06 Jul 2019 00:50:01: 18000000 INFO @ Sat, 06 Jul 2019 00:50:05: 16000000 INFO @ Sat, 06 Jul 2019 00:50:06: 16000000 INFO @ Sat, 06 Jul 2019 00:50:11: 19000000 INFO @ Sat, 06 Jul 2019 00:50:16: 17000000 INFO @ Sat, 06 Jul 2019 00:50:17: 17000000 INFO @ Sat, 06 Jul 2019 00:50:20: 20000000 INFO @ Sat, 06 Jul 2019 00:50:27: 18000000 INFO @ Sat, 06 Jul 2019 00:50:27: 18000000 INFO @ Sat, 06 Jul 2019 00:50:29: 21000000 INFO @ Sat, 06 Jul 2019 00:50:38: 19000000 INFO @ Sat, 06 Jul 2019 00:50:38: 19000000 INFO @ Sat, 06 Jul 2019 00:50:39: 22000000 INFO @ Sat, 06 Jul 2019 00:50:48: 23000000 INFO @ Sat, 06 Jul 2019 00:50:49: 20000000 INFO @ Sat, 06 Jul 2019 00:50:49: 20000000 INFO @ Sat, 06 Jul 2019 00:50:57: 24000000 INFO @ Sat, 06 Jul 2019 00:50:59: 21000000 INFO @ Sat, 06 Jul 2019 00:51:00: 21000000 INFO @ Sat, 06 Jul 2019 00:51:07: 25000000 INFO @ Sat, 06 Jul 2019 00:51:10: 22000000 INFO @ Sat, 06 Jul 2019 00:51:11: 22000000 INFO @ Sat, 06 Jul 2019 00:51:16: 26000000 INFO @ Sat, 06 Jul 2019 00:51:21: 23000000 INFO @ Sat, 06 Jul 2019 00:51:21: 23000000 INFO @ Sat, 06 Jul 2019 00:51:25: 27000000 INFO @ Sat, 06 Jul 2019 00:51:31: 24000000 INFO @ Sat, 06 Jul 2019 00:51:32: 24000000 INFO @ Sat, 06 Jul 2019 00:51:35: 28000000 INFO @ Sat, 06 Jul 2019 00:51:42: 25000000 INFO @ Sat, 06 Jul 2019 00:51:43: 25000000 INFO @ Sat, 06 Jul 2019 00:51:44: 29000000 INFO @ Sat, 06 Jul 2019 00:51:53: 26000000 INFO @ Sat, 06 Jul 2019 00:51:53: 30000000 INFO @ Sat, 06 Jul 2019 00:51:53: 26000000 INFO @ Sat, 06 Jul 2019 00:52:02: 31000000 INFO @ Sat, 06 Jul 2019 00:52:04: 27000000 INFO @ Sat, 06 Jul 2019 00:52:04: 27000000 INFO @ Sat, 06 Jul 2019 00:52:12: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 00:52:15: 28000000 INFO @ Sat, 06 Jul 2019 00:52:15: 28000000 INFO @ Sat, 06 Jul 2019 00:52:21: 33000000 INFO @ Sat, 06 Jul 2019 00:52:25: 29000000 INFO @ Sat, 06 Jul 2019 00:52:26: 29000000 INFO @ Sat, 06 Jul 2019 00:52:30: 34000000 INFO @ Sat, 06 Jul 2019 00:52:36: 30000000 INFO @ Sat, 06 Jul 2019 00:52:36: 30000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 00:52:40: 35000000 INFO @ Sat, 06 Jul 2019 00:52:47: 31000000 INFO @ Sat, 06 Jul 2019 00:52:47: 31000000 INFO @ Sat, 06 Jul 2019 00:52:49: 36000000 INFO @ Sat, 06 Jul 2019 00:52:58: 37000000 INFO @ Sat, 06 Jul 2019 00:52:58: 32000000 INFO @ Sat, 06 Jul 2019 00:52:58: 32000000 INFO @ Sat, 06 Jul 2019 00:53:07: 38000000 INFO @ Sat, 06 Jul 2019 00:53:09: 33000000 INFO @ Sat, 06 Jul 2019 00:53:09: 33000000 INFO @ Sat, 06 Jul 2019 00:53:16: 39000000 INFO @ Sat, 06 Jul 2019 00:53:20: 34000000 INFO @ Sat, 06 Jul 2019 00:53:20: 34000000 INFO @ Sat, 06 Jul 2019 00:53:25: 40000000 INFO @ Sat, 06 Jul 2019 00:53:30: 35000000 INFO @ Sat, 06 Jul 2019 00:53:31: 35000000 INFO @ Sat, 06 Jul 2019 00:53:34: 41000000 INFO @ Sat, 06 Jul 2019 00:53:41: 36000000 INFO @ Sat, 06 Jul 2019 00:53:41: 36000000 INFO @ Sat, 06 Jul 2019 00:53:44: 42000000 INFO @ Sat, 06 Jul 2019 00:53:51: 37000000 INFO @ Sat, 06 Jul 2019 00:53:51: 37000000 INFO @ Sat, 06 Jul 2019 00:53:53: 43000000 INFO @ Sat, 06 Jul 2019 00:54:02: 38000000 INFO @ Sat, 06 Jul 2019 00:54:02: 38000000 INFO @ Sat, 06 Jul 2019 00:54:02: 44000000 INFO @ Sat, 06 Jul 2019 00:54:12: 45000000 INFO @ Sat, 06 Jul 2019 00:54:12: 39000000 INFO @ Sat, 06 Jul 2019 00:54:12: 39000000 INFO @ Sat, 06 Jul 2019 00:54:21: 46000000 INFO @ Sat, 06 Jul 2019 00:54:23: 40000000 INFO @ Sat, 06 Jul 2019 00:54:23: 40000000 INFO @ Sat, 06 Jul 2019 00:54:30: 47000000 INFO @ Sat, 06 Jul 2019 00:54:34: 41000000 INFO @ Sat, 06 Jul 2019 00:54:34: 41000000 INFO @ Sat, 06 Jul 2019 00:54:39: 48000000 INFO @ Sat, 06 Jul 2019 00:54:44: 42000000 INFO @ Sat, 06 Jul 2019 00:54:45: 42000000 INFO @ Sat, 06 Jul 2019 00:54:49: 49000000 INFO @ Sat, 06 Jul 2019 00:54:55: 43000000 INFO @ Sat, 06 Jul 2019 00:54:56: 43000000 INFO @ Sat, 06 Jul 2019 00:54:58: 50000000 INFO @ Sat, 06 Jul 2019 00:55:06: 44000000 INFO @ Sat, 06 Jul 2019 00:55:07: 44000000 INFO @ Sat, 06 Jul 2019 00:55:07: 51000000 INFO @ Sat, 06 Jul 2019 00:55:16: 52000000 INFO @ Sat, 06 Jul 2019 00:55:17: 45000000 INFO @ Sat, 06 Jul 2019 00:55:17: 45000000 INFO @ Sat, 06 Jul 2019 00:55:26: 53000000 INFO @ Sat, 06 Jul 2019 00:55:28: 46000000 INFO @ Sat, 06 Jul 2019 00:55:28: 46000000 INFO @ Sat, 06 Jul 2019 00:55:35: 54000000 INFO @ Sat, 06 Jul 2019 00:55:39: 47000000 INFO @ Sat, 06 Jul 2019 00:55:39: 47000000 INFO @ Sat, 06 Jul 2019 00:55:44: 55000000 INFO @ Sat, 06 Jul 2019 00:55:49: 48000000 INFO @ Sat, 06 Jul 2019 00:55:50: 48000000 INFO @ Sat, 06 Jul 2019 00:55:53: 56000000 INFO @ Sat, 06 Jul 2019 00:56:00: 49000000 INFO @ Sat, 06 Jul 2019 00:56:00: 49000000 INFO @ Sat, 06 Jul 2019 00:56:03: 57000000 INFO @ Sat, 06 Jul 2019 00:56:03: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:56:03: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:56:03: #1 total tags in treatment: 26861646 INFO @ Sat, 06 Jul 2019 00:56:03: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:56:04: #1 tags after filtering in treatment: 14355177 INFO @ Sat, 06 Jul 2019 00:56:04: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 06 Jul 2019 00:56:04: #1 finished! INFO @ Sat, 06 Jul 2019 00:56:04: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:56:05: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:56:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:56:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 00:56:11: 50000000 INFO @ Sat, 06 Jul 2019 00:56:11: 50000000 INFO @ Sat, 06 Jul 2019 00:56:22: 51000000 INFO @ Sat, 06 Jul 2019 00:56:22: 51000000 INFO @ Sat, 06 Jul 2019 00:56:32: 52000000 INFO @ Sat, 06 Jul 2019 00:56:32: 52000000 INFO @ Sat, 06 Jul 2019 00:56:43: 53000000 INFO @ Sat, 06 Jul 2019 00:56:43: 53000000 INFO @ Sat, 06 Jul 2019 00:56:54: 54000000 INFO @ Sat, 06 Jul 2019 00:56:54: 54000000 INFO @ Sat, 06 Jul 2019 00:57:05: 55000000 INFO @ Sat, 06 Jul 2019 00:57:05: 55000000 INFO @ Sat, 06 Jul 2019 00:57:15: 56000000 INFO @ Sat, 06 Jul 2019 00:57:16: 56000000 INFO @ Sat, 06 Jul 2019 00:57:26: 57000000 INFO @ Sat, 06 Jul 2019 00:57:26: 57000000 INFO @ Sat, 06 Jul 2019 00:57:26: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:57:26: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:57:26: #1 total tags in treatment: 26861646 INFO @ Sat, 06 Jul 2019 00:57:26: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:57:26: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 00:57:26: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 00:57:26: #1 total tags in treatment: 26861646 INFO @ Sat, 06 Jul 2019 00:57:26: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 00:57:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 00:57:27: #1 tags after filtering in treatment: 14355177 INFO @ Sat, 06 Jul 2019 00:57:27: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 06 Jul 2019 00:57:27: #1 finished! INFO @ Sat, 06 Jul 2019 00:57:27: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:57:27: #1 tags after filtering in treatment: 14355177 INFO @ Sat, 06 Jul 2019 00:57:27: #1 Redundant rate of treatment: 0.47 INFO @ Sat, 06 Jul 2019 00:57:27: #1 finished! INFO @ Sat, 06 Jul 2019 00:57:27: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 00:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 00:57:28: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:57:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:57:28: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 00:57:28: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 00:57:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 00:57:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX381218/SRX381218.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling