Job ID = 2010488 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,057,709 reads read : 17,057,709 reads written : 17,057,709 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:12 17057709 reads; of these: 17057709 (100.00%) were unpaired; of these: 7754686 (45.46%) aligned 0 times 7543995 (44.23%) aligned exactly 1 time 1759028 (10.31%) aligned >1 times 54.54% overall alignment rate Time searching: 00:02:12 Overall time: 00:02:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5699296 / 9303023 = 0.6126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:57:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:57:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:57:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:57:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:57:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:57:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:57:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:57:25: 1000000 INFO @ Fri, 05 Jul 2019 22:57:25: 1000000 INFO @ Fri, 05 Jul 2019 22:57:27: 1000000 INFO @ Fri, 05 Jul 2019 22:57:36: 2000000 INFO @ Fri, 05 Jul 2019 22:57:40: 2000000 INFO @ Fri, 05 Jul 2019 22:57:40: 2000000 INFO @ Fri, 05 Jul 2019 22:57:47: 3000000 INFO @ Fri, 05 Jul 2019 22:57:54: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:57:54: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:57:54: #1 total tags in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:57:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:57:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:57:54: #1 tags after filtering in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:57:54: #1 finished! INFO @ Fri, 05 Jul 2019 22:57:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:57:54: #2 number of paired peaks: 43 WARNING @ Fri, 05 Jul 2019 22:57:54: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:57:54: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:57:54: 3000000 INFO @ Fri, 05 Jul 2019 22:57:56: 3000000 INFO @ Fri, 05 Jul 2019 22:58:02: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:58:02: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:58:02: #1 total tags in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:58:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:58:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:58:02: #1 tags after filtering in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:58:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:58:02: #1 finished! INFO @ Fri, 05 Jul 2019 22:58:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:58:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:58:03: #2 number of paired peaks: 43 WARNING @ Fri, 05 Jul 2019 22:58:03: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:58:03: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:58:04: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:58:04: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:58:04: #1 total tags in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:58:04: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:58:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:58:05: #1 tags after filtering in treatment: 3603727 INFO @ Fri, 05 Jul 2019 22:58:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:58:05: #1 finished! INFO @ Fri, 05 Jul 2019 22:58:05: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:58:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:58:05: #2 number of paired peaks: 43 WARNING @ Fri, 05 Jul 2019 22:58:05: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:58:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms)pass1 - making usageList (0 chroms): 14 millis : 14 millis pass1 - making usageList (0 chroms): 15 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377169/SRX377169.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。