Job ID = 2010482 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,959,204 reads read : 13,959,204 reads written : 13,959,204 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029320.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:45 13959204 reads; of these: 13959204 (100.00%) were unpaired; of these: 8860663 (63.48%) aligned 0 times 4223038 (30.25%) aligned exactly 1 time 875503 (6.27%) aligned >1 times 36.52% overall alignment rate Time searching: 00:01:45 Overall time: 00:01:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2935720 / 5098541 = 0.5758 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:46:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:46:56: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:46:56: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:46:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:46:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:46:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:46:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:46:58: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:46:58: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:47:04: 1000000 INFO @ Fri, 05 Jul 2019 22:47:04: 1000000 INFO @ Fri, 05 Jul 2019 22:47:06: 1000000 INFO @ Fri, 05 Jul 2019 22:47:11: 2000000 INFO @ Fri, 05 Jul 2019 22:47:12: 2000000 INFO @ Fri, 05 Jul 2019 22:47:12: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:47:12: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:47:12: #1 total tags in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:12: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:47:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:47:12: #1 tags after filtering in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:47:12: #1 finished! INFO @ Fri, 05 Jul 2019 22:47:12: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:47:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:47:13: #2 number of paired peaks: 66 WARNING @ Fri, 05 Jul 2019 22:47:13: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:47:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 22:47:13: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:47:13: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:47:13: #1 total tags in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:13: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:47:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:47:13: #1 tags after filtering in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:47:13: #1 finished! INFO @ Fri, 05 Jul 2019 22:47:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:47:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:47:13: #2 number of paired peaks: 66 WARNING @ Fri, 05 Jul 2019 22:47:13: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:47:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:47:14: 2000000 INFO @ Fri, 05 Jul 2019 22:47:15: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:47:15: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:47:15: #1 total tags in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:47:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:47:15: #1 tags after filtering in treatment: 2162821 INFO @ Fri, 05 Jul 2019 22:47:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:47:15: #1 finished! INFO @ Fri, 05 Jul 2019 22:47:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:47:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:47:16: #2 number of paired peaks: 66 WARNING @ Fri, 05 Jul 2019 22:47:16: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:47:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377163/SRX377163.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。