Job ID = 2010470 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,009,366 reads read : 17,009,366 reads written : 17,009,366 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:09 17009366 reads; of these: 17009366 (100.00%) were unpaired; of these: 8459512 (49.73%) aligned 0 times 6714372 (39.47%) aligned exactly 1 time 1835482 (10.79%) aligned >1 times 50.27% overall alignment rate Time searching: 00:02:09 Overall time: 00:02:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3839688 / 8549854 = 0.4491 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:52:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:52:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:52:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:52:10: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:52:10: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:52:19: 1000000 INFO @ Fri, 05 Jul 2019 22:52:20: 1000000 INFO @ Fri, 05 Jul 2019 22:52:30: 2000000 INFO @ Fri, 05 Jul 2019 22:52:31: 2000000 INFO @ Fri, 05 Jul 2019 22:52:40: 3000000 INFO @ Fri, 05 Jul 2019 22:52:42: 3000000 INFO @ Fri, 05 Jul 2019 22:52:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:52:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:52:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:52:51: 4000000 INFO @ Fri, 05 Jul 2019 22:52:53: 4000000 INFO @ Fri, 05 Jul 2019 22:52:53: 1000000 INFO @ Fri, 05 Jul 2019 22:52:58: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:52:58: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:52:58: #1 total tags in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:52:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:52:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:52:58: #1 tags after filtering in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:52:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:52:58: #1 finished! INFO @ Fri, 05 Jul 2019 22:52:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:52:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:52:59: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:52:59: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:52:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:53:00: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:53:00: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:53:00: #1 total tags in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:53:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:53:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:53:00: #1 tags after filtering in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:53:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:53:00: #1 finished! INFO @ Fri, 05 Jul 2019 22:53:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:53:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:53:00: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:53:00: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:53:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:53:02: 2000000 INFO @ Fri, 05 Jul 2019 22:53:10: 3000000 INFO @ Fri, 05 Jul 2019 22:53:18: 4000000 INFO @ Fri, 05 Jul 2019 22:53:24: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:53:24: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:53:24: #1 total tags in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:53:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:53:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:53:24: #1 tags after filtering in treatment: 4710166 INFO @ Fri, 05 Jul 2019 22:53:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:53:24: #1 finished! INFO @ Fri, 05 Jul 2019 22:53:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:53:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:53:24: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:53:24: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:53:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377152/SRX377152.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。