Job ID = 2010466 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,220,289 reads read : 16,220,289 reads written : 16,220,289 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 16220289 reads; of these: 16220289 (100.00%) were unpaired; of these: 10942699 (67.46%) aligned 0 times 4349030 (26.81%) aligned exactly 1 time 928560 (5.72%) aligned >1 times 32.54% overall alignment rate Time searching: 00:01:41 Overall time: 00:01:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3064675 / 5277590 = 0.5807 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:50:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:45: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:45: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:51: 1000000 INFO @ Fri, 05 Jul 2019 22:50:51: 1000000 INFO @ Fri, 05 Jul 2019 22:50:53: 1000000 INFO @ Fri, 05 Jul 2019 22:50:58: 2000000 INFO @ Fri, 05 Jul 2019 22:50:59: 2000000 INFO @ Fri, 05 Jul 2019 22:51:00: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:51:00: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:51:00: #1 total tags in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:51:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:51:00: #1 tags after filtering in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:51:00: #1 finished! INFO @ Fri, 05 Jul 2019 22:51:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:51:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:51:00: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:51:00: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:51:00: #1 total tags in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:51:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:51:00: #2 number of paired peaks: 64 WARNING @ Fri, 05 Jul 2019 22:51:00: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:51:00: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:51:00: #1 tags after filtering in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:51:00: #1 finished! INFO @ Fri, 05 Jul 2019 22:51:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:51:00: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:51:00: #2 number of paired peaks: 64 WARNING @ Fri, 05 Jul 2019 22:51:00: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:51:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:51:01: 2000000 INFO @ Fri, 05 Jul 2019 22:51:02: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:51:02: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:51:02: #1 total tags in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:51:02: #1 tags after filtering in treatment: 2212915 INFO @ Fri, 05 Jul 2019 22:51:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:51:02: #1 finished! INFO @ Fri, 05 Jul 2019 22:51:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:51:03: #2 number of paired peaks: 64 WARNING @ Fri, 05 Jul 2019 22:51:03: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:51:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377149/SRX377149.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。