Job ID = 2010465 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,632,152 reads read : 13,632,152 reads written : 13,632,152 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029305.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:04 13632152 reads; of these: 13632152 (100.00%) were unpaired; of these: 4770064 (34.99%) aligned 0 times 7282068 (53.42%) aligned exactly 1 time 1580020 (11.59%) aligned >1 times 65.01% overall alignment rate Time searching: 00:02:04 Overall time: 00:02:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 6474810 / 8862088 = 0.7306 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:44:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:44:01: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:44:01: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:44:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:44:02: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:44:02: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:44:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:44:04: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:44:04: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:44:10: 1000000 INFO @ Fri, 05 Jul 2019 22:44:13: 1000000 INFO @ Fri, 05 Jul 2019 22:44:13: 1000000 INFO @ Fri, 05 Jul 2019 22:44:17: 2000000 INFO @ Fri, 05 Jul 2019 22:44:20: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:44:20: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:44:20: #1 total tags in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:44:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:44:20: #1 tags after filtering in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:44:20: #1 finished! INFO @ Fri, 05 Jul 2019 22:44:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:44:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:44:20: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 22:44:20: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:44:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:44:23: 2000000 INFO @ Fri, 05 Jul 2019 22:44:23: 2000000 INFO @ Fri, 05 Jul 2019 22:44:26: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:44:26: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:44:26: #1 total tags in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:44:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:44:26: #1 tags after filtering in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:44:26: #1 finished! INFO @ Fri, 05 Jul 2019 22:44:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:44:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:44:26: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 22:44:26: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:44:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:44:27: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:44:27: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:44:27: #1 total tags in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:44:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:44:27: #1 tags after filtering in treatment: 2387278 INFO @ Fri, 05 Jul 2019 22:44:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:44:27: #1 finished! INFO @ Fri, 05 Jul 2019 22:44:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:44:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:44:27: #2 number of paired peaks: 58 WARNING @ Fri, 05 Jul 2019 22:44:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377148/SRX377148.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。