Job ID = 2010428 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,328,966 reads read : 13,328,966 reads written : 13,328,966 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 13328966 reads; of these: 13328966 (100.00%) were unpaired; of these: 2997830 (22.49%) aligned 0 times 9081820 (68.14%) aligned exactly 1 time 1249316 (9.37%) aligned >1 times 77.51% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7706848 / 10331136 = 0.7460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:50:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:50:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:50:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:50:28: 1000000 INFO @ Fri, 05 Jul 2019 22:50:28: 1000000 INFO @ Fri, 05 Jul 2019 22:50:29: 1000000 INFO @ Fri, 05 Jul 2019 22:50:37: 2000000 INFO @ Fri, 05 Jul 2019 22:50:37: 2000000 INFO @ Fri, 05 Jul 2019 22:50:37: 2000000 INFO @ Fri, 05 Jul 2019 22:50:42: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:50:42: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:50:42: #1 total tags in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:42: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:50:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:50:42: #1 tags after filtering in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:50:42: #1 finished! INFO @ Fri, 05 Jul 2019 22:50:42: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:50:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:50:42: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 22:50:42: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:50:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:50:43: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:50:43: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:50:43: #1 total tags in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:50:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:50:43: #1 tags after filtering in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:50:43: #1 finished! INFO @ Fri, 05 Jul 2019 22:50:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:50:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:50:43: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 22:50:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:50:43: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:50:43: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:50:43: #1 total tags in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:50:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:50:43: #1 tags after filtering in treatment: 2624288 INFO @ Fri, 05 Jul 2019 22:50:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:50:43: #1 finished! INFO @ Fri, 05 Jul 2019 22:50:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:50:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:50:43: #2 number of paired peaks: 30 WARNING @ Fri, 05 Jul 2019 22:50:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377114/SRX377114.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。