Job ID = 2010423 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,428,728 reads read : 8,428,728 reads written : 8,428,728 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 8428728 reads; of these: 8428728 (100.00%) were unpaired; of these: 5535023 (65.67%) aligned 0 times 2507044 (29.74%) aligned exactly 1 time 386661 (4.59%) aligned >1 times 34.33% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1007518 / 2893705 = 0.3482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:36:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:36:57: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:36:57: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:36:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:36:58: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:36:58: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:36:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:36:59: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:36:59: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:37:06: 1000000 INFO @ Fri, 05 Jul 2019 22:37:06: 1000000 INFO @ Fri, 05 Jul 2019 22:37:08: 1000000 INFO @ Fri, 05 Jul 2019 22:37:13: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:37:13: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:37:13: #1 total tags in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:13: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:37:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:37:13: #1 tags after filtering in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:37:13: #1 finished! INFO @ Fri, 05 Jul 2019 22:37:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:37:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:37:14: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:37:14: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:37:14: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:37:14: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:37:14: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:37:14: #1 total tags in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:14: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:37:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:37:14: #1 tags after filtering in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:37:14: #1 finished! INFO @ Fri, 05 Jul 2019 22:37:14: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:37:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:37:14: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:37:14: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:37:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:37:16: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:37:16: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:37:16: #1 total tags in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:37:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:37:16: #1 tags after filtering in treatment: 1886187 INFO @ Fri, 05 Jul 2019 22:37:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:37:16: #1 finished! INFO @ Fri, 05 Jul 2019 22:37:16: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:37:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:37:16: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:37:16: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:37:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377109/SRX377109.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。