Job ID = 2010413 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,315,061 reads read : 5,315,061 reads written : 5,315,061 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029245.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:50 5315061 reads; of these: 5315061 (100.00%) were unpaired; of these: 315658 (5.94%) aligned 0 times 4112203 (77.37%) aligned exactly 1 time 887200 (16.69%) aligned >1 times 94.06% overall alignment rate Time searching: 00:00:50 Overall time: 00:00:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1209405 / 4999403 = 0.2419 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:31:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:31:50: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:31:50: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:31:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:31:51: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:31:51: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:31:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:31:52: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:31:52: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:31:58: 1000000 INFO @ Fri, 05 Jul 2019 22:31:59: 1000000 INFO @ Fri, 05 Jul 2019 22:31:59: 1000000 INFO @ Fri, 05 Jul 2019 22:32:05: 2000000 INFO @ Fri, 05 Jul 2019 22:32:06: 2000000 INFO @ Fri, 05 Jul 2019 22:32:07: 2000000 INFO @ Fri, 05 Jul 2019 22:32:13: 3000000 INFO @ Fri, 05 Jul 2019 22:32:13: 3000000 INFO @ Fri, 05 Jul 2019 22:32:14: 3000000 INFO @ Fri, 05 Jul 2019 22:32:18: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:32:18: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:32:18: #1 total tags in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:32:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:32:18: #1 tags after filtering in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:32:18: #1 finished! INFO @ Fri, 05 Jul 2019 22:32:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:32:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:32:19: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:32:19: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:32:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 22:32:19: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:32:19: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:32:19: #1 total tags in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:19: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:32:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:32:19: #1 tags after filtering in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:32:19: #1 finished! INFO @ Fri, 05 Jul 2019 22:32:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:32:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:32:19: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:32:19: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:32:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:32:20: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:32:20: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:32:20: #1 total tags in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:32:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:32:20: #1 tags after filtering in treatment: 3789998 INFO @ Fri, 05 Jul 2019 22:32:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:32:20: #1 finished! INFO @ Fri, 05 Jul 2019 22:32:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:32:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:32:20: #2 number of paired peaks: 27 WARNING @ Fri, 05 Jul 2019 22:32:20: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:32:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377088/SRX377088.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。