Job ID = 2640845 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,498,708 reads read : 17,498,708 reads written : 17,498,708 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029231.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 17498708 reads; of these: 17498708 (100.00%) were unpaired; of these: 2696776 (15.41%) aligned 0 times 12523728 (71.57%) aligned exactly 1 time 2278204 (13.02%) aligned >1 times 84.59% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6642987 / 14801932 = 0.4488 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:34:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:34:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:34:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:34:10: 1000000 INFO @ Sat, 24 Aug 2019 19:34:20: 2000000 INFO @ Sat, 24 Aug 2019 19:34:29: 3000000 INFO @ Sat, 24 Aug 2019 19:34:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:34:31: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:34:31: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:34:38: 4000000 INFO @ Sat, 24 Aug 2019 19:34:40: 1000000 INFO @ Sat, 24 Aug 2019 19:34:47: 5000000 INFO @ Sat, 24 Aug 2019 19:34:49: 2000000 INFO @ Sat, 24 Aug 2019 19:34:56: 6000000 INFO @ Sat, 24 Aug 2019 19:34:56: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:35:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:35:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:35:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:35:04: 4000000 INFO @ Sat, 24 Aug 2019 19:35:05: 7000000 INFO @ Sat, 24 Aug 2019 19:35:11: 1000000 INFO @ Sat, 24 Aug 2019 19:35:12: 5000000 INFO @ Sat, 24 Aug 2019 19:35:15: 8000000 INFO @ Sat, 24 Aug 2019 19:35:16: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:35:16: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:35:16: #1 total tags in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:35:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:35:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:35:16: #1 tags after filtering in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:35:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:35:16: #1 finished! INFO @ Sat, 24 Aug 2019 19:35:16: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:35:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:35:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:35:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:35:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:35:19: 6000000 INFO @ Sat, 24 Aug 2019 19:35:20: 2000000 INFO @ Sat, 24 Aug 2019 19:35:27: 7000000 INFO @ Sat, 24 Aug 2019 19:35:30: 3000000 INFO @ Sat, 24 Aug 2019 19:35:34: 8000000 INFO @ Sat, 24 Aug 2019 19:35:35: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:35:35: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:35:35: #1 total tags in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:35:35: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:35:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:35:35: #1 tags after filtering in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:35:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:35:35: #1 finished! INFO @ Sat, 24 Aug 2019 19:35:35: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:35:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:35:36: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:35:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:35:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:35:39: 4000000 INFO @ Sat, 24 Aug 2019 19:35:48: 5000000 INFO @ Sat, 24 Aug 2019 19:35:57: 6000000 INFO @ Sat, 24 Aug 2019 19:36:06: 7000000 INFO @ Sat, 24 Aug 2019 19:36:15: 8000000 INFO @ Sat, 24 Aug 2019 19:36:16: #1 tag size is determined as 50 bps INFO @ Sat, 24 Aug 2019 19:36:16: #1 tag size = 50 INFO @ Sat, 24 Aug 2019 19:36:16: #1 total tags in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:36:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:36:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:36:16: #1 tags after filtering in treatment: 8158945 INFO @ Sat, 24 Aug 2019 19:36:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:36:16: #1 finished! INFO @ Sat, 24 Aug 2019 19:36:16: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:36:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:36:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:36:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:36:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377074/SRX377074.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。