Job ID = 2010397 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,551,622 reads read : 15,551,622 reads written : 15,551,622 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029227.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 15551622 reads; of these: 15551622 (100.00%) were unpaired; of these: 1759001 (11.31%) aligned 0 times 11747874 (75.54%) aligned exactly 1 time 2044747 (13.15%) aligned >1 times 88.69% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6810097 / 13792621 = 0.4937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:37:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:37:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:37:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:37:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:37:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:37:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:37:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:37:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:37:47: 1000000 INFO @ Fri, 05 Jul 2019 22:37:48: 1000000 INFO @ Fri, 05 Jul 2019 22:37:50: 1000000 INFO @ Fri, 05 Jul 2019 22:37:55: 2000000 INFO @ Fri, 05 Jul 2019 22:37:58: 2000000 INFO @ Fri, 05 Jul 2019 22:38:00: 2000000 INFO @ Fri, 05 Jul 2019 22:38:02: 3000000 INFO @ Fri, 05 Jul 2019 22:38:07: 3000000 INFO @ Fri, 05 Jul 2019 22:38:10: 3000000 INFO @ Fri, 05 Jul 2019 22:38:11: 4000000 INFO @ Fri, 05 Jul 2019 22:38:18: 4000000 INFO @ Fri, 05 Jul 2019 22:38:19: 5000000 INFO @ Fri, 05 Jul 2019 22:38:20: 4000000 INFO @ Fri, 05 Jul 2019 22:38:28: 6000000 INFO @ Fri, 05 Jul 2019 22:38:28: 5000000 INFO @ Fri, 05 Jul 2019 22:38:30: 5000000 INFO @ Fri, 05 Jul 2019 22:38:35: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:38:35: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:38:35: #1 total tags in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:38:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:38:36: #1 tags after filtering in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:38:36: #1 finished! INFO @ Fri, 05 Jul 2019 22:38:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:38:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:38:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:38:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:38:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:38:38: 6000000 INFO @ Fri, 05 Jul 2019 22:38:41: 6000000 INFO @ Fri, 05 Jul 2019 22:38:48: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:38:48: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:38:48: #1 total tags in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:38:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:38:48: #1 tags after filtering in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:38:48: #1 finished! INFO @ Fri, 05 Jul 2019 22:38:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:38:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:38:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:38:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:38:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:38:50: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:38:50: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:38:50: #1 total tags in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:38:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:38:50: #1 tags after filtering in treatment: 6982524 INFO @ Fri, 05 Jul 2019 22:38:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:38:50: #1 finished! INFO @ Fri, 05 Jul 2019 22:38:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:38:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:38:51: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:38:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:38:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377070/SRX377070.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。