Job ID = 2010395 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,626,033 reads read : 15,626,033 reads written : 15,626,033 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029225.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 15626033 reads; of these: 15626033 (100.00%) were unpaired; of these: 3582697 (22.93%) aligned 0 times 10252152 (65.61%) aligned exactly 1 time 1791184 (11.46%) aligned >1 times 77.07% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6525599 / 12043336 = 0.5418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:32:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:32:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:32:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:32:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:32:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:32:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:32:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:32:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:32:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:32:29: 1000000 INFO @ Fri, 05 Jul 2019 22:32:30: 1000000 INFO @ Fri, 05 Jul 2019 22:32:32: 1000000 INFO @ Fri, 05 Jul 2019 22:32:38: 2000000 INFO @ Fri, 05 Jul 2019 22:32:39: 2000000 INFO @ Fri, 05 Jul 2019 22:32:43: 2000000 INFO @ Fri, 05 Jul 2019 22:32:46: 3000000 INFO @ Fri, 05 Jul 2019 22:32:47: 3000000 INFO @ Fri, 05 Jul 2019 22:32:54: 3000000 INFO @ Fri, 05 Jul 2019 22:32:55: 4000000 INFO @ Fri, 05 Jul 2019 22:32:56: 4000000 INFO @ Fri, 05 Jul 2019 22:33:03: 5000000 INFO @ Fri, 05 Jul 2019 22:33:04: 5000000 INFO @ Fri, 05 Jul 2019 22:33:04: 4000000 INFO @ Fri, 05 Jul 2019 22:33:08: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:33:08: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:33:08: #1 total tags in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:33:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:33:08: #1 tags after filtering in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:33:08: #1 finished! INFO @ Fri, 05 Jul 2019 22:33:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:33:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:33:08: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:33:08: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:33:08: #1 total tags in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:33:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:33:08: #1 tags after filtering in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:33:08: #1 finished! INFO @ Fri, 05 Jul 2019 22:33:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:33:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:33:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:33:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:33:08: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:33:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:33:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:33:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:33:14: 5000000 INFO @ Fri, 05 Jul 2019 22:33:19: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:33:19: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:33:19: #1 total tags in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:19: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:33:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:33:19: #1 tags after filtering in treatment: 5517737 INFO @ Fri, 05 Jul 2019 22:33:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:33:19: #1 finished! INFO @ Fri, 05 Jul 2019 22:33:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:33:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:33:19: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:33:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:33:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377068/SRX377068.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。