Job ID = 4306413 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,990,099 reads read : 5,990,099 reads written : 5,990,099 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029207.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 5990099 reads; of these: 5990099 (100.00%) were unpaired; of these: 382633 (6.39%) aligned 0 times 4485671 (74.88%) aligned exactly 1 time 1121795 (18.73%) aligned >1 times 93.61% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1883270 / 5607466 = 0.3359 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 22:36:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 22:36:19: #1 read tag files... INFO @ Thu, 12 Dec 2019 22:36:19: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 22:36:26: 1000000 INFO @ Thu, 12 Dec 2019 22:36:33: 2000000 INFO @ Thu, 12 Dec 2019 22:36:40: 3000000 INFO @ Thu, 12 Dec 2019 22:36:45: #1 tag size is determined as 36 bps INFO @ Thu, 12 Dec 2019 22:36:45: #1 tag size = 36 INFO @ Thu, 12 Dec 2019 22:36:45: #1 total tags in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:36:45: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 22:36:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 22:36:45: #1 tags after filtering in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:36:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 22:36:45: #1 finished! INFO @ Thu, 12 Dec 2019 22:36:45: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 22:36:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 22:36:45: #2 number of paired peaks: 28 WARNING @ Thu, 12 Dec 2019 22:36:45: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 22:36:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 22:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 22:36:49: #1 read tag files... INFO @ Thu, 12 Dec 2019 22:36:49: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 22:36:56: 1000000 INFO @ Thu, 12 Dec 2019 22:37:03: 2000000 INFO @ Thu, 12 Dec 2019 22:37:10: 3000000 INFO @ Thu, 12 Dec 2019 22:37:15: #1 tag size is determined as 36 bps INFO @ Thu, 12 Dec 2019 22:37:15: #1 tag size = 36 INFO @ Thu, 12 Dec 2019 22:37:15: #1 total tags in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:37:15: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 22:37:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 22:37:15: #1 tags after filtering in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:37:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 22:37:15: #1 finished! INFO @ Thu, 12 Dec 2019 22:37:15: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 22:37:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 22:37:16: #2 number of paired peaks: 28 WARNING @ Thu, 12 Dec 2019 22:37:16: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 22:37:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Thu, 12 Dec 2019 22:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 22:37:19: #1 read tag files... INFO @ Thu, 12 Dec 2019 22:37:19: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 22:37:26: 1000000 INFO @ Thu, 12 Dec 2019 22:37:34: 2000000 INFO @ Thu, 12 Dec 2019 22:37:41: 3000000 INFO @ Thu, 12 Dec 2019 22:37:46: #1 tag size is determined as 36 bps INFO @ Thu, 12 Dec 2019 22:37:46: #1 tag size = 36 INFO @ Thu, 12 Dec 2019 22:37:46: #1 total tags in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:37:46: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 22:37:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 22:37:46: #1 tags after filtering in treatment: 3724196 INFO @ Thu, 12 Dec 2019 22:37:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 22:37:46: #1 finished! INFO @ Thu, 12 Dec 2019 22:37:46: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 22:37:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 22:37:46: #2 number of paired peaks: 28 WARNING @ Thu, 12 Dec 2019 22:37:46: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 22:37:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377050/SRX377050.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。