Job ID = 2010369 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,797,787 reads read : 7,797,787 reads written : 7,797,787 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1029179.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:14 7797787 reads; of these: 7797787 (100.00%) were unpaired; of these: 520005 (6.67%) aligned 0 times 6402734 (82.11%) aligned exactly 1 time 875048 (11.22%) aligned >1 times 93.33% overall alignment rate Time searching: 00:01:14 Overall time: 00:01:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3648263 / 7277782 = 0.5013 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:17:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:17:48: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:17:48: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:17:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:17:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:17:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:17:50: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:17:50: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:17:57: 1000000 INFO @ Fri, 05 Jul 2019 22:17:57: 1000000 INFO @ Fri, 05 Jul 2019 22:17:57: 1000000 INFO @ Fri, 05 Jul 2019 22:18:04: 2000000 INFO @ Fri, 05 Jul 2019 22:18:06: 2000000 INFO @ Fri, 05 Jul 2019 22:18:06: 2000000 INFO @ Fri, 05 Jul 2019 22:18:12: 3000000 INFO @ Fri, 05 Jul 2019 22:18:15: 3000000 INFO @ Fri, 05 Jul 2019 22:18:15: 3000000 INFO @ Fri, 05 Jul 2019 22:18:16: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:18:16: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:18:16: #1 total tags in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:18:16: #1 tags after filtering in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:18:16: #1 finished! INFO @ Fri, 05 Jul 2019 22:18:16: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:18:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:18:16: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:18:16: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:18:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:18:20: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:18:20: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:18:20: #1 total tags in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:18:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:18:20: #1 tags after filtering in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:18:20: #1 finished! INFO @ Fri, 05 Jul 2019 22:18:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:18:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:18:20: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 22:18:20: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 22:18:20: #1 total tags in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:18:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:18:20: #1 tags after filtering in treatment: 3629519 INFO @ Fri, 05 Jul 2019 22:18:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:18:20: #1 finished! INFO @ Fri, 05 Jul 2019 22:18:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:18:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:18:20: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:18:20: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:18:20: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:18:21: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:18:21: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:18:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05_*.xls’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX377022/SRX377022.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。