Job ID = 10851107 sra ファイルのダウンロード中... Completed: 336696K bytes transferred in 6 seconds (456574K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 19190458 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3748398/SRR6789148.sra Written 19190458 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3748398/SRR6789148.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:38 19190458 reads; of these: 19190458 (100.00%) were unpaired; of these: 5872482 (30.60%) aligned 0 times 10972014 (57.17%) aligned exactly 1 time 2345962 (12.22%) aligned >1 times 69.40% overall alignment rate Time searching: 00:03:38 Overall time: 00:03:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4878269 / 13317976 = 0.3663 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 05 Jul 2018 22:52:45: # Command line: callpeak -t SRX3748398.bam -f BAM -g 12100000 -n SRX3748398.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3748398.20 # format = BAM # ChIP-seq file = ['SRX3748398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Jul 2018 22:52:45: #1 read tag files... INFO @ Thu, 05 Jul 2018 22:52:45: #1 read treatment tags... INFO @ Thu, 05 Jul 2018 22:52:45: # Command line: callpeak -t SRX3748398.bam -f BAM -g 12100000 -n SRX3748398.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3748398.10 # format = BAM # ChIP-seq file = ['SRX3748398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Jul 2018 22:52:45: #1 read tag files... INFO @ Thu, 05 Jul 2018 22:52:45: #1 read treatment tags... INFO @ Thu, 05 Jul 2018 22:52:45: # Command line: callpeak -t SRX3748398.bam -f BAM -g 12100000 -n SRX3748398.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3748398.05 # format = BAM # ChIP-seq file = ['SRX3748398.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Jul 2018 22:52:45: #1 read tag files... INFO @ Thu, 05 Jul 2018 22:52:45: #1 read treatment tags... INFO @ Thu, 05 Jul 2018 22:52:51: 1000000 INFO @ Thu, 05 Jul 2018 22:52:51: 1000000 INFO @ Thu, 05 Jul 2018 22:52:51: 1000000 INFO @ Thu, 05 Jul 2018 22:52:57: 2000000 INFO @ Thu, 05 Jul 2018 22:52:58: 2000000 INFO @ Thu, 05 Jul 2018 22:52:58: 2000000 INFO @ Thu, 05 Jul 2018 22:53:04: 3000000 INFO @ Thu, 05 Jul 2018 22:53:04: 3000000 INFO @ Thu, 05 Jul 2018 22:53:04: 3000000 INFO @ Thu, 05 Jul 2018 22:53:10: 4000000 INFO @ Thu, 05 Jul 2018 22:53:10: 4000000 INFO @ Thu, 05 Jul 2018 22:53:10: 4000000 INFO @ Thu, 05 Jul 2018 22:53:16: 5000000 INFO @ Thu, 05 Jul 2018 22:53:16: 5000000 INFO @ Thu, 05 Jul 2018 22:53:17: 5000000 INFO @ Thu, 05 Jul 2018 22:53:22: 6000000 INFO @ Thu, 05 Jul 2018 22:53:22: 6000000 INFO @ Thu, 05 Jul 2018 22:53:23: 6000000 INFO @ Thu, 05 Jul 2018 22:53:28: 7000000 INFO @ Thu, 05 Jul 2018 22:53:28: 7000000 INFO @ Thu, 05 Jul 2018 22:53:30: 7000000 INFO @ Thu, 05 Jul 2018 22:53:34: 8000000 INFO @ Thu, 05 Jul 2018 22:53:34: 8000000 INFO @ Thu, 05 Jul 2018 22:53:36: 8000000 INFO @ Thu, 05 Jul 2018 22:53:36: #1 tag size is determined as 51 bps INFO @ Thu, 05 Jul 2018 22:53:36: #1 tag size = 51 INFO @ Thu, 05 Jul 2018 22:53:36: #1 total tags in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:36: #1 user defined the maximum tags... INFO @ Thu, 05 Jul 2018 22:53:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Jul 2018 22:53:37: #1 tags after filtering in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Jul 2018 22:53:37: #1 finished! INFO @ Thu, 05 Jul 2018 22:53:37: #2 Build Peak Model... INFO @ Thu, 05 Jul 2018 22:53:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Jul 2018 22:53:37: #1 tag size is determined as 51 bps INFO @ Thu, 05 Jul 2018 22:53:37: #1 tag size = 51 INFO @ Thu, 05 Jul 2018 22:53:37: #1 total tags in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:37: #1 user defined the maximum tags... INFO @ Thu, 05 Jul 2018 22:53:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Jul 2018 22:53:37: #2 number of paired peaks: 0 WARNING @ Thu, 05 Jul 2018 22:53:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Jul 2018 22:53:37: Process for pairing-model is terminated! cat: SRX3748398.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3748398.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 05 Jul 2018 22:53:37: #1 tags after filtering in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Jul 2018 22:53:37: #1 finished! INFO @ Thu, 05 Jul 2018 22:53:37: #2 Build Peak Model... INFO @ Thu, 05 Jul 2018 22:53:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Jul 2018 22:53:38: #2 number of paired peaks: 0 WARNING @ Thu, 05 Jul 2018 22:53:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Jul 2018 22:53:38: Process for pairing-model is terminated! cat: SRX3748398.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3748398.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 05 Jul 2018 22:53:39: #1 tag size is determined as 51 bps INFO @ Thu, 05 Jul 2018 22:53:39: #1 tag size = 51 INFO @ Thu, 05 Jul 2018 22:53:39: #1 total tags in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:39: #1 user defined the maximum tags... INFO @ Thu, 05 Jul 2018 22:53:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Jul 2018 22:53:40: #1 tags after filtering in treatment: 8439707 INFO @ Thu, 05 Jul 2018 22:53:40: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Jul 2018 22:53:40: #1 finished! INFO @ Thu, 05 Jul 2018 22:53:40: #2 Build Peak Model... INFO @ Thu, 05 Jul 2018 22:53:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Jul 2018 22:53:40: #2 number of paired peaks: 0 WARNING @ Thu, 05 Jul 2018 22:53:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Jul 2018 22:53:40: Process for pairing-model is terminated! cat: SRX3748398.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3748398.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3748398.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。