Job ID = 11162637 sra ファイルのダウンロード中... Completed: 47199K bytes transferred in 3 seconds (114376K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 1556536 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3709389/SRR6736441.sra Written 1556536 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3709389/SRR6736441.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 1556536 reads; of these: 1556536 (100.00%) were paired; of these: 326209 (20.96%) aligned concordantly 0 times 841738 (54.08%) aligned concordantly exactly 1 time 388589 (24.96%) aligned concordantly >1 times ---- 326209 pairs aligned concordantly 0 times; of these: 197 (0.06%) aligned discordantly 1 time ---- 326012 pairs aligned 0 times concordantly or discordantly; of these: 652024 mates make up the pairs; of these: 574697 (88.14%) aligned 0 times 52090 (7.99%) aligned exactly 1 time 25237 (3.87%) aligned >1 times 81.54% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1042226 / 1230420 = 0.8470 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:39:44: # Command line: callpeak -t SRX3709389.bam -f BAM -g 12100000 -n SRX3709389.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3709389.05 # format = BAM # ChIP-seq file = ['SRX3709389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:39:44: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:39:44: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:39:44: # Command line: callpeak -t SRX3709389.bam -f BAM -g 12100000 -n SRX3709389.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3709389.10 # format = BAM # ChIP-seq file = ['SRX3709389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:39:44: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:39:44: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:39:44: # Command line: callpeak -t SRX3709389.bam -f BAM -g 12100000 -n SRX3709389.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3709389.20 # format = BAM # ChIP-seq file = ['SRX3709389.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:39:44: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:39:44: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size is determined as 40 bps INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size = 40 INFO @ Wed, 05 Sep 2018 10:39:47: #1 total tags in treatment: 188148 INFO @ Wed, 05 Sep 2018 10:39:47: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:39:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:39:47: #1 tags after filtering in treatment: 135484 INFO @ Wed, 05 Sep 2018 10:39:47: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 05 Sep 2018 10:39:47: #1 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #2 number of paired peaks: 362 WARNING @ Wed, 05 Sep 2018 10:39:47: Fewer paired peaks (362) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 362 pairs to build model! INFO @ Wed, 05 Sep 2018 10:39:47: start model_add_line... INFO @ Wed, 05 Sep 2018 10:39:47: start X-correlation... INFO @ Wed, 05 Sep 2018 10:39:47: end of X-cor INFO @ Wed, 05 Sep 2018 10:39:47: #2 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 predicted fragment length is 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2 alternative fragment length(s) may be 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2.2 Generate R script for model : SRX3709389.20_model.r INFO @ Wed, 05 Sep 2018 10:39:47: #3 Call peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size is determined as 40 bps INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size = 40 INFO @ Wed, 05 Sep 2018 10:39:47: #1 total tags in treatment: 188148 INFO @ Wed, 05 Sep 2018 10:39:47: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:39:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:39:47: #1 tags after filtering in treatment: 135484 INFO @ Wed, 05 Sep 2018 10:39:47: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 05 Sep 2018 10:39:47: #1 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #2 number of paired peaks: 362 WARNING @ Wed, 05 Sep 2018 10:39:47: Fewer paired peaks (362) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 362 pairs to build model! INFO @ Wed, 05 Sep 2018 10:39:47: start model_add_line... INFO @ Wed, 05 Sep 2018 10:39:47: start X-correlation... INFO @ Wed, 05 Sep 2018 10:39:47: end of X-cor INFO @ Wed, 05 Sep 2018 10:39:47: #2 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 predicted fragment length is 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2 alternative fragment length(s) may be 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2.2 Generate R script for model : SRX3709389.05_model.r INFO @ Wed, 05 Sep 2018 10:39:47: #3 Call peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size is determined as 40 bps INFO @ Wed, 05 Sep 2018 10:39:47: #1 tag size = 40 INFO @ Wed, 05 Sep 2018 10:39:47: #1 total tags in treatment: 188148 INFO @ Wed, 05 Sep 2018 10:39:47: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:39:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:39:47: #1 tags after filtering in treatment: 135484 INFO @ Wed, 05 Sep 2018 10:39:47: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 05 Sep 2018 10:39:47: #1 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:39:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #2 number of paired peaks: 362 WARNING @ Wed, 05 Sep 2018 10:39:47: Fewer paired peaks (362) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 362 pairs to build model! INFO @ Wed, 05 Sep 2018 10:39:47: start model_add_line... INFO @ Wed, 05 Sep 2018 10:39:47: start X-correlation... INFO @ Wed, 05 Sep 2018 10:39:47: end of X-cor INFO @ Wed, 05 Sep 2018 10:39:47: #2 finished! INFO @ Wed, 05 Sep 2018 10:39:47: #2 predicted fragment length is 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2 alternative fragment length(s) may be 170 bps INFO @ Wed, 05 Sep 2018 10:39:47: #2.2 Generate R script for model : SRX3709389.10_model.r INFO @ Wed, 05 Sep 2018 10:39:47: #3 Call peaks... INFO @ Wed, 05 Sep 2018 10:39:47: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 05 Sep 2018 10:39:47: #3 Call peaks for each chromosome... INFO @ Wed, 05 Sep 2018 10:39:48: #3 Call peaks for each chromosome... INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write output xls file... SRX3709389.20_peaks.xls INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write peak in narrowPeak format file... SRX3709389.20_peaks.narrowPeak INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write summits bed file... SRX3709389.20_summits.bed INFO @ Wed, 05 Sep 2018 10:39:48: Done! INFO @ Wed, 05 Sep 2018 10:39:48: #3 Call peaks for each chromosome... pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (184 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write output xls file... SRX3709389.05_peaks.xls INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write peak in narrowPeak format file... SRX3709389.05_peaks.narrowPeak INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write summits bed file... SRX3709389.05_summits.bed INFO @ Wed, 05 Sep 2018 10:39:48: Done! INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write output xls file... SRX3709389.10_peaks.xls pass1 - making usageList (16 chroms): 3 millis INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write peak in narrowPeak format file... SRX3709389.10_peaks.narrowPeak pass2 - checking and writing primary data (418 records, 4 fields): 4 millis INFO @ Wed, 05 Sep 2018 10:39:48: #4 Write summits bed file... SRX3709389.10_summits.bed INFO @ Wed, 05 Sep 2018 10:39:48: Done! CompletedMACS2peakCalling pass1 - making usageList (16 chroms): 2 millis pass2 - checking and writing primary data (330 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。