Job ID = 14520606 SRX = SRX3659094 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6047829 spots for SRR6682810/SRR6682810.sra Written 6047829 spots for SRR6682810/SRR6682810.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:30 6047829 reads; of these: 6047829 (100.00%) were paired; of these: 709937 (11.74%) aligned concordantly 0 times 4256244 (70.38%) aligned concordantly exactly 1 time 1081648 (17.88%) aligned concordantly >1 times ---- 709937 pairs aligned concordantly 0 times; of these: 102831 (14.48%) aligned discordantly 1 time ---- 607106 pairs aligned 0 times concordantly or discordantly; of these: 1214212 mates make up the pairs; of these: 1083714 (89.25%) aligned 0 times 59552 (4.90%) aligned exactly 1 time 70946 (5.84%) aligned >1 times 91.04% overall alignment rate Time searching: 00:05:30 Overall time: 00:05:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 309524 / 5428344 = 0.0570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:07: 1000000 INFO @ Sat, 15 Jan 2022 19:39:13: 2000000 INFO @ Sat, 15 Jan 2022 19:39:19: 3000000 INFO @ Sat, 15 Jan 2022 19:39:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:31: 5000000 INFO @ Sat, 15 Jan 2022 19:39:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:36: 6000000 INFO @ Sat, 15 Jan 2022 19:39:37: 1000000 INFO @ Sat, 15 Jan 2022 19:39:42: 7000000 INFO @ Sat, 15 Jan 2022 19:39:44: 2000000 INFO @ Sat, 15 Jan 2022 19:39:49: 8000000 INFO @ Sat, 15 Jan 2022 19:39:50: 3000000 INFO @ Sat, 15 Jan 2022 19:39:55: 9000000 INFO @ Sat, 15 Jan 2022 19:39:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:40:02: 10000000 INFO @ Sat, 15 Jan 2022 19:40:03: 5000000 INFO @ Sat, 15 Jan 2022 19:40:05: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:40:05: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:40:05: #1 total tags in treatment: 5030392 INFO @ Sat, 15 Jan 2022 19:40:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:05: #1 tags after filtering in treatment: 3856720 INFO @ Sat, 15 Jan 2022 19:40:05: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:40:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:05: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:40:05: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:40:08: 1000000 INFO @ Sat, 15 Jan 2022 19:40:10: 6000000 INFO @ Sat, 15 Jan 2022 19:40:14: 2000000 INFO @ Sat, 15 Jan 2022 19:40:16: 7000000 INFO @ Sat, 15 Jan 2022 19:40:20: 3000000 INFO @ Sat, 15 Jan 2022 19:40:22: 8000000 INFO @ Sat, 15 Jan 2022 19:40:27: 4000000 INFO @ Sat, 15 Jan 2022 19:40:28: 9000000 INFO @ Sat, 15 Jan 2022 19:40:33: 5000000 INFO @ Sat, 15 Jan 2022 19:40:34: 10000000 INFO @ Sat, 15 Jan 2022 19:40:37: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:40:37: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:40:37: #1 total tags in treatment: 5030392 INFO @ Sat, 15 Jan 2022 19:40:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:37: #1 tags after filtering in treatment: 3856720 INFO @ Sat, 15 Jan 2022 19:40:37: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:40:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:37: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:40:37: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:40:39: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:40:45: 7000000 INFO @ Sat, 15 Jan 2022 19:40:51: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:40:57: 9000000 INFO @ Sat, 15 Jan 2022 19:41:03: 10000000 INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:41:05: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:41:05: #1 total tags in treatment: 5030392 INFO @ Sat, 15 Jan 2022 19:41:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:41:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:41:05: #1 tags after filtering in treatment: 3856720 INFO @ Sat, 15 Jan 2022 19:41:05: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:41:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:41:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:41:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:41:05: #2 number of paired peaks: 39 WARNING @ Sat, 15 Jan 2022 19:41:05: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:41:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659094/SRX3659094.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling