Job ID = 14520544 SRX = SRX3659076 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7050063 spots for SRR6682791/SRR6682791.sra Written 7050063 spots for SRR6682791/SRR6682791.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:28 7050063 reads; of these: 7050063 (100.00%) were paired; of these: 174617 (2.48%) aligned concordantly 0 times 6111277 (86.68%) aligned concordantly exactly 1 time 764169 (10.84%) aligned concordantly >1 times ---- 174617 pairs aligned concordantly 0 times; of these: 50100 (28.69%) aligned discordantly 1 time ---- 124517 pairs aligned 0 times concordantly or discordantly; of these: 249034 mates make up the pairs; of these: 153474 (61.63%) aligned 0 times 72834 (29.25%) aligned exactly 1 time 22726 (9.13%) aligned >1 times 98.91% overall alignment rate Time searching: 00:09:28 Overall time: 00:09:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 774705 / 6841458 = 0.1132 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:40:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:40:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:40:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:07: 1000000 INFO @ Sat, 15 Jan 2022 19:41:18: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:41:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:41:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:41:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:30: 3000000 INFO @ Sat, 15 Jan 2022 19:41:37: 1000000 INFO @ Sat, 15 Jan 2022 19:41:41: 4000000 INFO @ Sat, 15 Jan 2022 19:41:48: 2000000 INFO @ Sat, 15 Jan 2022 19:41:53: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:41:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:41:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:41:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:59: 3000000 INFO @ Sat, 15 Jan 2022 19:42:04: 6000000 INFO @ Sat, 15 Jan 2022 19:42:07: 1000000 INFO @ Sat, 15 Jan 2022 19:42:11: 4000000 INFO @ Sat, 15 Jan 2022 19:42:16: 7000000 INFO @ Sat, 15 Jan 2022 19:42:17: 2000000 INFO @ Sat, 15 Jan 2022 19:42:22: 5000000 INFO @ Sat, 15 Jan 2022 19:42:28: 8000000 INFO @ Sat, 15 Jan 2022 19:42:28: 3000000 INFO @ Sat, 15 Jan 2022 19:42:34: 6000000 INFO @ Sat, 15 Jan 2022 19:42:39: 4000000 INFO @ Sat, 15 Jan 2022 19:42:39: 9000000 INFO @ Sat, 15 Jan 2022 19:42:45: 7000000 INFO @ Sat, 15 Jan 2022 19:42:49: 5000000 INFO @ Sat, 15 Jan 2022 19:42:51: 10000000 INFO @ Sat, 15 Jan 2022 19:42:56: 8000000 INFO @ Sat, 15 Jan 2022 19:43:00: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:43:03: 11000000 INFO @ Sat, 15 Jan 2022 19:43:08: 9000000 INFO @ Sat, 15 Jan 2022 19:43:11: 7000000 INFO @ Sat, 15 Jan 2022 19:43:15: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:43:19: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:43:19: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:43:19: #1 total tags in treatment: 6101153 INFO @ Sat, 15 Jan 2022 19:43:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:19: 10000000 INFO @ Sat, 15 Jan 2022 19:43:19: #1 tags after filtering in treatment: 3845435 INFO @ Sat, 15 Jan 2022 19:43:19: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:43:19: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:43:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:43:22: 8000000 INFO @ Sat, 15 Jan 2022 19:43:31: 11000000 INFO @ Sat, 15 Jan 2022 19:43:32: 9000000 INFO @ Sat, 15 Jan 2022 19:43:42: 12000000 INFO @ Sat, 15 Jan 2022 19:43:42: 10000000 INFO @ Sat, 15 Jan 2022 19:43:46: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:43:46: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:43:46: #1 total tags in treatment: 6101153 INFO @ Sat, 15 Jan 2022 19:43:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:46: #1 tags after filtering in treatment: 3845435 INFO @ Sat, 15 Jan 2022 19:43:46: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:43:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:43:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:43:53: 11000000 INFO @ Sat, 15 Jan 2022 19:44:02: 12000000 INFO @ Sat, 15 Jan 2022 19:44:06: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:44:06: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:44:06: #1 total tags in treatment: 6101153 INFO @ Sat, 15 Jan 2022 19:44:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:44:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:44:06: #1 tags after filtering in treatment: 3845435 INFO @ Sat, 15 Jan 2022 19:44:06: #1 Redundant rate of treatment: 0.37 INFO @ Sat, 15 Jan 2022 19:44:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:44:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:44:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:44:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:44:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:44:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659076/SRX3659076.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling