Job ID = 14520539 SRX = SRX3659072 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11844005 spots for SRR6682787/SRR6682787.sra Written 11844005 spots for SRR6682787/SRR6682787.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:29 11844005 reads; of these: 11844005 (100.00%) were paired; of these: 365332 (3.08%) aligned concordantly 0 times 10648604 (89.91%) aligned concordantly exactly 1 time 830069 (7.01%) aligned concordantly >1 times ---- 365332 pairs aligned concordantly 0 times; of these: 87178 (23.86%) aligned discordantly 1 time ---- 278154 pairs aligned 0 times concordantly or discordantly; of these: 556308 mates make up the pairs; of these: 382878 (68.82%) aligned 0 times 146939 (26.41%) aligned exactly 1 time 26491 (4.76%) aligned >1 times 98.38% overall alignment rate Time searching: 00:15:29 Overall time: 00:15:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2154911 / 11342689 = 0.1900 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:01: 1000000 INFO @ Sat, 15 Jan 2022 19:52:08: 2000000 INFO @ Sat, 15 Jan 2022 19:52:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:22: 4000000 INFO @ Sat, 15 Jan 2022 19:52:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:29: 5000000 INFO @ Sat, 15 Jan 2022 19:52:32: 1000000 INFO @ Sat, 15 Jan 2022 19:52:37: 6000000 INFO @ Sat, 15 Jan 2022 19:52:39: 2000000 INFO @ Sat, 15 Jan 2022 19:52:45: 7000000 INFO @ Sat, 15 Jan 2022 19:52:47: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:52: 8000000 INFO @ Sat, 15 Jan 2022 19:52:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:54: 4000000 INFO @ Sat, 15 Jan 2022 19:53:00: 9000000 INFO @ Sat, 15 Jan 2022 19:53:02: 1000000 INFO @ Sat, 15 Jan 2022 19:53:02: 5000000 INFO @ Sat, 15 Jan 2022 19:53:08: 10000000 INFO @ Sat, 15 Jan 2022 19:53:10: 6000000 INFO @ Sat, 15 Jan 2022 19:53:11: 2000000 INFO @ Sat, 15 Jan 2022 19:53:16: 11000000 INFO @ Sat, 15 Jan 2022 19:53:18: 7000000 INFO @ Sat, 15 Jan 2022 19:53:19: 3000000 INFO @ Sat, 15 Jan 2022 19:53:24: 12000000 INFO @ Sat, 15 Jan 2022 19:53:25: 8000000 INFO @ Sat, 15 Jan 2022 19:53:27: 4000000 INFO @ Sat, 15 Jan 2022 19:53:31: 13000000 INFO @ Sat, 15 Jan 2022 19:53:33: 9000000 INFO @ Sat, 15 Jan 2022 19:53:35: 5000000 INFO @ Sat, 15 Jan 2022 19:53:39: 14000000 INFO @ Sat, 15 Jan 2022 19:53:40: 10000000 INFO @ Sat, 15 Jan 2022 19:53:43: 6000000 INFO @ Sat, 15 Jan 2022 19:53:47: 15000000 INFO @ Sat, 15 Jan 2022 19:53:48: 11000000 INFO @ Sat, 15 Jan 2022 19:53:51: 7000000 INFO @ Sat, 15 Jan 2022 19:53:55: 16000000 INFO @ Sat, 15 Jan 2022 19:53:55: 12000000 INFO @ Sat, 15 Jan 2022 19:53:59: 8000000 INFO @ Sat, 15 Jan 2022 19:54:02: 17000000 INFO @ Sat, 15 Jan 2022 19:54:03: 13000000 INFO @ Sat, 15 Jan 2022 19:54:07: 9000000 INFO @ Sat, 15 Jan 2022 19:54:10: 18000000 INFO @ Sat, 15 Jan 2022 19:54:11: 14000000 INFO @ Sat, 15 Jan 2022 19:54:15: 10000000 INFO @ Sat, 15 Jan 2022 19:54:18: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:54:18: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:54:18: #1 total tags in treatment: 9325979 INFO @ Sat, 15 Jan 2022 19:54:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:18: #1 tags after filtering in treatment: 5111262 INFO @ Sat, 15 Jan 2022 19:54:18: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 19:54:18: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:54:18: 15000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:54:23: 11000000 INFO @ Sat, 15 Jan 2022 19:54:25: 16000000 INFO @ Sat, 15 Jan 2022 19:54:32: 12000000 INFO @ Sat, 15 Jan 2022 19:54:32: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:54:39: 18000000 INFO @ Sat, 15 Jan 2022 19:54:41: 13000000 INFO @ Sat, 15 Jan 2022 19:54:46: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:54:46: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:54:46: #1 total tags in treatment: 9325979 INFO @ Sat, 15 Jan 2022 19:54:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:46: #1 tags after filtering in treatment: 5111262 INFO @ Sat, 15 Jan 2022 19:54:46: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 19:54:46: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:54:49: 14000000 INFO @ Sat, 15 Jan 2022 19:54:56: 15000000 INFO @ Sat, 15 Jan 2022 19:55:04: 16000000 INFO @ Sat, 15 Jan 2022 19:55:13: 17000000 INFO @ Sat, 15 Jan 2022 19:55:21: 18000000 INFO @ Sat, 15 Jan 2022 19:55:28: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:55:28: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:55:28: #1 total tags in treatment: 9325979 INFO @ Sat, 15 Jan 2022 19:55:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:29: #1 tags after filtering in treatment: 5111262 INFO @ Sat, 15 Jan 2022 19:55:29: #1 Redundant rate of treatment: 0.45 INFO @ Sat, 15 Jan 2022 19:55:29: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659072/SRX3659072.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling