Job ID = 14520474 SRX = SRX3659054 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9131550 spots for SRR6682751/SRR6682751.sra Written 9131550 spots for SRR6682751/SRR6682751.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:11 9131550 reads; of these: 9131550 (100.00%) were paired; of these: 707887 (7.75%) aligned concordantly 0 times 7822320 (85.66%) aligned concordantly exactly 1 time 601343 (6.59%) aligned concordantly >1 times ---- 707887 pairs aligned concordantly 0 times; of these: 402091 (56.80%) aligned discordantly 1 time ---- 305796 pairs aligned 0 times concordantly or discordantly; of these: 611592 mates make up the pairs; of these: 468667 (76.63%) aligned 0 times 78995 (12.92%) aligned exactly 1 time 63930 (10.45%) aligned >1 times 97.43% overall alignment rate Time searching: 00:14:11 Overall time: 00:14:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 785246 / 8762238 = 0.0896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:38:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:38:31: #1 read treatment tags... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:03: 1000000 INFO @ Sat, 15 Jan 2022 19:39:16: 2000000 INFO @ Sat, 15 Jan 2022 19:39:19: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:39:29: 3000000 INFO @ Sat, 15 Jan 2022 19:39:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:39:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:39:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:39:36: 2000000 INFO @ Sat, 15 Jan 2022 19:39:44: 1000000 INFO @ Sat, 15 Jan 2022 19:39:45: 4000000 INFO @ Sat, 15 Jan 2022 19:39:52: 3000000 INFO @ Sat, 15 Jan 2022 19:39:58: 2000000 INFO @ Sat, 15 Jan 2022 19:40:00: 5000000 INFO @ Sat, 15 Jan 2022 19:40:09: 4000000 INFO @ Sat, 15 Jan 2022 19:40:12: 3000000 INFO @ Sat, 15 Jan 2022 19:40:17: 6000000 INFO @ Sat, 15 Jan 2022 19:40:24: 5000000 INFO @ Sat, 15 Jan 2022 19:40:25: 4000000 INFO @ Sat, 15 Jan 2022 19:40:33: 7000000 INFO @ Sat, 15 Jan 2022 19:40:40: 5000000 INFO @ Sat, 15 Jan 2022 19:40:40: 6000000 INFO @ Sat, 15 Jan 2022 19:40:51: 8000000 INFO @ Sat, 15 Jan 2022 19:40:54: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:40:57: 7000000 INFO @ Sat, 15 Jan 2022 19:41:07: 9000000 INFO @ Sat, 15 Jan 2022 19:41:09: 7000000 INFO @ Sat, 15 Jan 2022 19:41:14: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:41:25: 8000000 INFO @ Sat, 15 Jan 2022 19:41:26: 10000000 INFO @ Sat, 15 Jan 2022 19:41:32: 9000000 INFO @ Sat, 15 Jan 2022 19:41:40: 9000000 INFO @ Sat, 15 Jan 2022 19:41:44: 11000000 INFO @ Sat, 15 Jan 2022 19:41:50: 10000000 INFO @ Sat, 15 Jan 2022 19:41:57: 10000000 INFO @ Sat, 15 Jan 2022 19:42:06: 12000000 INFO @ Sat, 15 Jan 2022 19:42:12: 11000000 INFO @ Sat, 15 Jan 2022 19:42:16: 11000000 INFO @ Sat, 15 Jan 2022 19:42:23: 13000000 INFO @ Sat, 15 Jan 2022 19:42:27: 12000000 INFO @ Sat, 15 Jan 2022 19:42:31: 12000000 INFO @ Sat, 15 Jan 2022 19:42:39: 14000000 INFO @ Sat, 15 Jan 2022 19:42:42: 13000000 INFO @ Sat, 15 Jan 2022 19:42:45: 13000000 INFO @ Sat, 15 Jan 2022 19:42:54: 15000000 INFO @ Sat, 15 Jan 2022 19:42:57: 14000000 INFO @ Sat, 15 Jan 2022 19:43:01: 14000000 INFO @ Sat, 15 Jan 2022 19:43:09: 16000000 INFO @ Sat, 15 Jan 2022 19:43:11: 15000000 INFO @ Sat, 15 Jan 2022 19:43:12: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:43:12: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:43:12: #1 total tags in treatment: 7653036 INFO @ Sat, 15 Jan 2022 19:43:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:12: #1 tags after filtering in treatment: 4633257 INFO @ Sat, 15 Jan 2022 19:43:12: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:43:12: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:43:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:43:15: 15000000 INFO @ Sat, 15 Jan 2022 19:43:25: 16000000 INFO @ Sat, 15 Jan 2022 19:43:28: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:43:28: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:43:28: #1 total tags in treatment: 7653036 INFO @ Sat, 15 Jan 2022 19:43:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:28: #1 tags after filtering in treatment: 4633257 INFO @ Sat, 15 Jan 2022 19:43:28: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:43:28: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:43:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:43:29: 16000000 INFO @ Sat, 15 Jan 2022 19:43:32: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:43:32: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:43:32: #1 total tags in treatment: 7653036 INFO @ Sat, 15 Jan 2022 19:43:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:32: #1 tags after filtering in treatment: 4633257 INFO @ Sat, 15 Jan 2022 19:43:32: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:43:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:43:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659054/SRX3659054.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling