Job ID = 14520473 SRX = SRX3659053 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10954623 spots for SRR6682750/SRR6682750.sra Written 10954623 spots for SRR6682750/SRR6682750.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:21 10954623 reads; of these: 10954623 (100.00%) were paired; of these: 849127 (7.75%) aligned concordantly 0 times 9283510 (84.75%) aligned concordantly exactly 1 time 821986 (7.50%) aligned concordantly >1 times ---- 849127 pairs aligned concordantly 0 times; of these: 496844 (58.51%) aligned discordantly 1 time ---- 352283 pairs aligned 0 times concordantly or discordantly; of these: 704566 mates make up the pairs; of these: 523478 (74.30%) aligned 0 times 91646 (13.01%) aligned exactly 1 time 89442 (12.69%) aligned >1 times 97.61% overall alignment rate Time searching: 00:13:21 Overall time: 00:13:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 966794 / 10523157 = 0.0919 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:25: 1000000 INFO @ Sat, 15 Jan 2022 19:36:34: 2000000 INFO @ Sat, 15 Jan 2022 19:36:43: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:52: 4000000 INFO @ Sat, 15 Jan 2022 19:36:56: 1000000 INFO @ Sat, 15 Jan 2022 19:37:02: 5000000 INFO @ Sat, 15 Jan 2022 19:37:06: 2000000 INFO @ Sat, 15 Jan 2022 19:37:12: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:16: 3000000 INFO @ Sat, 15 Jan 2022 19:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:22: 7000000 INFO @ Sat, 15 Jan 2022 19:37:24: 1000000 INFO @ Sat, 15 Jan 2022 19:37:27: 4000000 INFO @ Sat, 15 Jan 2022 19:37:32: 2000000 INFO @ Sat, 15 Jan 2022 19:37:33: 8000000 INFO @ Sat, 15 Jan 2022 19:37:37: 5000000 INFO @ Sat, 15 Jan 2022 19:37:40: 3000000 INFO @ Sat, 15 Jan 2022 19:37:43: 9000000 INFO @ Sat, 15 Jan 2022 19:37:47: 6000000 INFO @ Sat, 15 Jan 2022 19:37:47: 4000000 INFO @ Sat, 15 Jan 2022 19:37:54: 10000000 INFO @ Sat, 15 Jan 2022 19:37:55: 5000000 INFO @ Sat, 15 Jan 2022 19:37:58: 7000000 INFO @ Sat, 15 Jan 2022 19:38:02: 6000000 INFO @ Sat, 15 Jan 2022 19:38:04: 11000000 INFO @ Sat, 15 Jan 2022 19:38:08: 8000000 INFO @ Sat, 15 Jan 2022 19:38:10: 7000000 INFO @ Sat, 15 Jan 2022 19:38:15: 12000000 INFO @ Sat, 15 Jan 2022 19:38:18: 8000000 INFO @ Sat, 15 Jan 2022 19:38:19: 9000000 INFO @ Sat, 15 Jan 2022 19:38:25: 9000000 INFO @ Sat, 15 Jan 2022 19:38:25: 13000000 INFO @ Sat, 15 Jan 2022 19:38:29: 10000000 INFO @ Sat, 15 Jan 2022 19:38:33: 10000000 INFO @ Sat, 15 Jan 2022 19:38:35: 14000000 INFO @ Sat, 15 Jan 2022 19:38:39: 11000000 INFO @ Sat, 15 Jan 2022 19:38:40: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:38:45: 15000000 INFO @ Sat, 15 Jan 2022 19:38:48: 12000000 INFO @ Sat, 15 Jan 2022 19:38:49: 12000000 INFO @ Sat, 15 Jan 2022 19:38:55: 16000000 INFO @ Sat, 15 Jan 2022 19:38:55: 13000000 INFO @ Sat, 15 Jan 2022 19:38:59: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:39:03: 14000000 INFO @ Sat, 15 Jan 2022 19:39:05: 17000000 INFO @ Sat, 15 Jan 2022 19:39:09: 14000000 INFO @ Sat, 15 Jan 2022 19:39:11: 15000000 INFO @ Sat, 15 Jan 2022 19:39:16: 18000000 INFO @ Sat, 15 Jan 2022 19:39:18: 16000000 INFO @ Sat, 15 Jan 2022 19:39:20: 15000000 INFO @ Sat, 15 Jan 2022 19:39:26: 19000000 INFO @ Sat, 15 Jan 2022 19:39:26: 17000000 INFO @ Sat, 15 Jan 2022 19:39:30: 16000000 INFO @ Sat, 15 Jan 2022 19:39:31: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:39:31: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:39:31: #1 total tags in treatment: 9160081 INFO @ Sat, 15 Jan 2022 19:39:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:31: #1 tags after filtering in treatment: 5202658 INFO @ Sat, 15 Jan 2022 19:39:31: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:39:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:34: 18000000 INFO @ Sat, 15 Jan 2022 19:39:39: 17000000 INFO @ Sat, 15 Jan 2022 19:39:41: 19000000 INFO @ Sat, 15 Jan 2022 19:39:45: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:39:45: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:39:45: #1 total tags in treatment: 9160081 INFO @ Sat, 15 Jan 2022 19:39:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:39:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:39:45: #1 tags after filtering in treatment: 5202658 INFO @ Sat, 15 Jan 2022 19:39:45: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:39:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:39:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:39:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:39:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:39:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:39:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:39:48: 18000000 INFO @ Sat, 15 Jan 2022 19:39:57: 19000000 INFO @ Sat, 15 Jan 2022 19:40:00: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:40:00: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:40:00: #1 total tags in treatment: 9160081 INFO @ Sat, 15 Jan 2022 19:40:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:40:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:40:01: #1 tags after filtering in treatment: 5202658 INFO @ Sat, 15 Jan 2022 19:40:01: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:40:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:40:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:40:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:40:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:40:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:40:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659053/SRX3659053.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling