Job ID = 14520472 SRX = SRX3659052 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8410381 spots for SRR6682749/SRR6682749.sra Written 8410381 spots for SRR6682749/SRR6682749.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:24 8410381 reads; of these: 8410381 (100.00%) were paired; of these: 822787 (9.78%) aligned concordantly 0 times 7025645 (83.54%) aligned concordantly exactly 1 time 561949 (6.68%) aligned concordantly >1 times ---- 822787 pairs aligned concordantly 0 times; of these: 520917 (63.31%) aligned discordantly 1 time ---- 301870 pairs aligned 0 times concordantly or discordantly; of these: 603740 mates make up the pairs; of these: 448310 (74.26%) aligned 0 times 75354 (12.48%) aligned exactly 1 time 80076 (13.26%) aligned >1 times 97.33% overall alignment rate Time searching: 00:10:24 Overall time: 00:10:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 887683 / 8029749 = 0.1105 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:30:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:30:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:30:54: 1000000 INFO @ Sat, 15 Jan 2022 19:31:02: 2000000 INFO @ Sat, 15 Jan 2022 19:31:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:18: 4000000 INFO @ Sat, 15 Jan 2022 19:31:23: 1000000 INFO @ Sat, 15 Jan 2022 19:31:27: 5000000 INFO @ Sat, 15 Jan 2022 19:31:32: 2000000 INFO @ Sat, 15 Jan 2022 19:31:35: 6000000 INFO @ Sat, 15 Jan 2022 19:31:40: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:44: 7000000 INFO @ Sat, 15 Jan 2022 19:31:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:49: 4000000 INFO @ Sat, 15 Jan 2022 19:31:53: 8000000 INFO @ Sat, 15 Jan 2022 19:31:54: 1000000 INFO @ Sat, 15 Jan 2022 19:31:58: 5000000 INFO @ Sat, 15 Jan 2022 19:32:02: 9000000 INFO @ Sat, 15 Jan 2022 19:32:03: 2000000 INFO @ Sat, 15 Jan 2022 19:32:07: 6000000 INFO @ Sat, 15 Jan 2022 19:32:11: 10000000 INFO @ Sat, 15 Jan 2022 19:32:12: 3000000 INFO @ Sat, 15 Jan 2022 19:32:16: 7000000 INFO @ Sat, 15 Jan 2022 19:32:21: 11000000 INFO @ Sat, 15 Jan 2022 19:32:21: 4000000 INFO @ Sat, 15 Jan 2022 19:32:25: 8000000 INFO @ Sat, 15 Jan 2022 19:32:30: 12000000 INFO @ Sat, 15 Jan 2022 19:32:30: 5000000 INFO @ Sat, 15 Jan 2022 19:32:35: 9000000 INFO @ Sat, 15 Jan 2022 19:32:39: 13000000 INFO @ Sat, 15 Jan 2022 19:32:39: 6000000 INFO @ Sat, 15 Jan 2022 19:32:44: 10000000 INFO @ Sat, 15 Jan 2022 19:32:48: 14000000 INFO @ Sat, 15 Jan 2022 19:32:49: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:32:53: 11000000 INFO @ Sat, 15 Jan 2022 19:32:54: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:32:54: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:32:54: #1 total tags in treatment: 6726518 INFO @ Sat, 15 Jan 2022 19:32:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:32:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:32:54: #1 tags after filtering in treatment: 4001824 INFO @ Sat, 15 Jan 2022 19:32:54: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:32:54: #1 finished! INFO @ Sat, 15 Jan 2022 19:32:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:32:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:32:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:32:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:32:57: 8000000 INFO @ Sat, 15 Jan 2022 19:33:01: 12000000 INFO @ Sat, 15 Jan 2022 19:33:06: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:33:10: 13000000 INFO @ Sat, 15 Jan 2022 19:33:14: 10000000 INFO @ Sat, 15 Jan 2022 19:33:18: 14000000 INFO @ Sat, 15 Jan 2022 19:33:22: 11000000 INFO @ Sat, 15 Jan 2022 19:33:23: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:33:23: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:33:23: #1 total tags in treatment: 6726518 INFO @ Sat, 15 Jan 2022 19:33:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:23: #1 tags after filtering in treatment: 4001824 INFO @ Sat, 15 Jan 2022 19:33:23: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:33:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:33:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:33:30: 12000000 INFO @ Sat, 15 Jan 2022 19:33:37: 13000000 INFO @ Sat, 15 Jan 2022 19:33:45: 14000000 INFO @ Sat, 15 Jan 2022 19:33:50: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:33:50: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:33:50: #1 total tags in treatment: 6726518 INFO @ Sat, 15 Jan 2022 19:33:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:50: #1 tags after filtering in treatment: 4001824 INFO @ Sat, 15 Jan 2022 19:33:50: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:33:50: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:33:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659052/SRX3659052.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling