Job ID = 14520471 SRX = SRX3659051 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7927754 spots for SRR6682748/SRR6682748.sra Written 7927754 spots for SRR6682748/SRR6682748.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:25 7927754 reads; of these: 7927754 (100.00%) were paired; of these: 477738 (6.03%) aligned concordantly 0 times 6750421 (85.15%) aligned concordantly exactly 1 time 699595 (8.82%) aligned concordantly >1 times ---- 477738 pairs aligned concordantly 0 times; of these: 250376 (52.41%) aligned discordantly 1 time ---- 227362 pairs aligned 0 times concordantly or discordantly; of these: 454724 mates make up the pairs; of these: 341109 (75.01%) aligned 0 times 54622 (12.01%) aligned exactly 1 time 58993 (12.97%) aligned >1 times 97.85% overall alignment rate Time searching: 00:10:25 Overall time: 00:10:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 690486 / 7659236 = 0.0902 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:30:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:30:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:30:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:30:58: 1000000 INFO @ Sat, 15 Jan 2022 19:31:08: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:19: 3000000 INFO @ Sat, 15 Jan 2022 19:31:31: 1000000 INFO @ Sat, 15 Jan 2022 19:31:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:31:44: 5000000 INFO @ Sat, 15 Jan 2022 19:31:45: 2000000 INFO @ Sat, 15 Jan 2022 19:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:31:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:31:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:31:57: 6000000 INFO @ Sat, 15 Jan 2022 19:32:01: 3000000 INFO @ Sat, 15 Jan 2022 19:32:03: 1000000 INFO @ Sat, 15 Jan 2022 19:32:09: 7000000 INFO @ Sat, 15 Jan 2022 19:32:19: 4000000 INFO @ Sat, 15 Jan 2022 19:32:19: 2000000 INFO @ Sat, 15 Jan 2022 19:32:20: 8000000 INFO @ Sat, 15 Jan 2022 19:32:32: 9000000 INFO @ Sat, 15 Jan 2022 19:32:36: 5000000 INFO @ Sat, 15 Jan 2022 19:32:36: 3000000 INFO @ Sat, 15 Jan 2022 19:32:44: 10000000 INFO @ Sat, 15 Jan 2022 19:32:53: 6000000 INFO @ Sat, 15 Jan 2022 19:32:53: 4000000 INFO @ Sat, 15 Jan 2022 19:32:56: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:33:08: 12000000 INFO @ Sat, 15 Jan 2022 19:33:10: 5000000 INFO @ Sat, 15 Jan 2022 19:33:10: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:33:20: 13000000 INFO @ Sat, 15 Jan 2022 19:33:27: 6000000 INFO @ Sat, 15 Jan 2022 19:33:27: 8000000 INFO @ Sat, 15 Jan 2022 19:33:32: 14000000 INFO @ Sat, 15 Jan 2022 19:33:33: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:33:33: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:33:33: #1 total tags in treatment: 6767794 INFO @ Sat, 15 Jan 2022 19:33:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:33:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:33:33: #1 tags after filtering in treatment: 4172220 INFO @ Sat, 15 Jan 2022 19:33:33: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:33:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:33:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:33:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:33:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:33:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:33:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:33:43: 9000000 INFO @ Sat, 15 Jan 2022 19:33:44: 7000000 INFO @ Sat, 15 Jan 2022 19:34:00: 8000000 INFO @ Sat, 15 Jan 2022 19:34:00: 10000000 INFO @ Sat, 15 Jan 2022 19:34:16: 11000000 INFO @ Sat, 15 Jan 2022 19:34:16: 9000000 INFO @ Sat, 15 Jan 2022 19:34:33: 10000000 INFO @ Sat, 15 Jan 2022 19:34:33: 12000000 INFO @ Sat, 15 Jan 2022 19:34:50: 11000000 INFO @ Sat, 15 Jan 2022 19:34:51: 13000000 INFO @ Sat, 15 Jan 2022 19:35:07: 12000000 INFO @ Sat, 15 Jan 2022 19:35:09: 14000000 INFO @ Sat, 15 Jan 2022 19:35:10: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:35:10: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:35:10: #1 total tags in treatment: 6767794 INFO @ Sat, 15 Jan 2022 19:35:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:11: #1 tags after filtering in treatment: 4172220 INFO @ Sat, 15 Jan 2022 19:35:11: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:35:11: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:35:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:35:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:35:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 8 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:35:23: 13000000 INFO @ Sat, 15 Jan 2022 19:35:39: 14000000 INFO @ Sat, 15 Jan 2022 19:35:40: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:35:40: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:35:40: #1 total tags in treatment: 6767794 INFO @ Sat, 15 Jan 2022 19:35:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:41: #1 tags after filtering in treatment: 4172220 INFO @ Sat, 15 Jan 2022 19:35:41: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:35:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:35:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:35:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:35:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659051/SRX3659051.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling