Job ID = 14520449 SRX = SRX3659049 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10700688 spots for SRR6682746/SRR6682746.sra Written 10700688 spots for SRR6682746/SRR6682746.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:25 10700688 reads; of these: 10700688 (100.00%) were paired; of these: 739374 (6.91%) aligned concordantly 0 times 9250498 (86.45%) aligned concordantly exactly 1 time 710816 (6.64%) aligned concordantly >1 times ---- 739374 pairs aligned concordantly 0 times; of these: 409434 (55.38%) aligned discordantly 1 time ---- 329940 pairs aligned 0 times concordantly or discordantly; of these: 659880 mates make up the pairs; of these: 503664 (76.33%) aligned 0 times 90654 (13.74%) aligned exactly 1 time 65562 (9.94%) aligned >1 times 97.65% overall alignment rate Time searching: 00:13:25 Overall time: 00:13:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1087345 / 10303888 = 0.1055 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:32:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:32:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:32:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:32:54: 1000000 INFO @ Sat, 15 Jan 2022 19:33:03: 2000000 INFO @ Sat, 15 Jan 2022 19:33:12: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:21: 4000000 INFO @ Sat, 15 Jan 2022 19:33:24: 1000000 INFO @ Sat, 15 Jan 2022 19:33:30: 5000000 INFO @ Sat, 15 Jan 2022 19:33:34: 2000000 INFO @ Sat, 15 Jan 2022 19:33:39: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:43: 3000000 INFO @ Sat, 15 Jan 2022 19:33:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:49: 7000000 INFO @ Sat, 15 Jan 2022 19:33:52: 4000000 INFO @ Sat, 15 Jan 2022 19:33:53: 1000000 INFO @ Sat, 15 Jan 2022 19:33:58: 8000000 INFO @ Sat, 15 Jan 2022 19:34:01: 5000000 INFO @ Sat, 15 Jan 2022 19:34:02: 2000000 INFO @ Sat, 15 Jan 2022 19:34:08: 9000000 INFO @ Sat, 15 Jan 2022 19:34:10: 6000000 INFO @ Sat, 15 Jan 2022 19:34:11: 3000000 INFO @ Sat, 15 Jan 2022 19:34:17: 10000000 INFO @ Sat, 15 Jan 2022 19:34:19: 7000000 INFO @ Sat, 15 Jan 2022 19:34:20: 4000000 INFO @ Sat, 15 Jan 2022 19:34:26: 11000000 INFO @ Sat, 15 Jan 2022 19:34:28: 8000000 INFO @ Sat, 15 Jan 2022 19:34:29: 5000000 INFO @ Sat, 15 Jan 2022 19:34:35: 12000000 INFO @ Sat, 15 Jan 2022 19:34:37: 9000000 INFO @ Sat, 15 Jan 2022 19:34:38: 6000000 INFO @ Sat, 15 Jan 2022 19:34:44: 13000000 INFO @ Sat, 15 Jan 2022 19:34:46: 10000000 INFO @ Sat, 15 Jan 2022 19:34:47: 7000000 INFO @ Sat, 15 Jan 2022 19:34:53: 14000000 INFO @ Sat, 15 Jan 2022 19:34:55: 11000000 INFO @ Sat, 15 Jan 2022 19:34:56: 8000000 INFO @ Sat, 15 Jan 2022 19:35:02: 15000000 INFO @ Sat, 15 Jan 2022 19:35:04: 12000000 INFO @ Sat, 15 Jan 2022 19:35:05: 9000000 INFO @ Sat, 15 Jan 2022 19:35:11: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:35:13: 13000000 INFO @ Sat, 15 Jan 2022 19:35:14: 10000000 INFO @ Sat, 15 Jan 2022 19:35:20: 17000000 INFO @ Sat, 15 Jan 2022 19:35:22: 14000000 INFO @ Sat, 15 Jan 2022 19:35:23: 11000000 INFO @ Sat, 15 Jan 2022 19:35:29: 18000000 INFO @ Sat, 15 Jan 2022 19:35:32: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:35:32: 12000000 INFO @ Sat, 15 Jan 2022 19:35:35: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:35:35: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:35:35: #1 total tags in treatment: 8890961 INFO @ Sat, 15 Jan 2022 19:35:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:35:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:35:36: #1 tags after filtering in treatment: 5210675 INFO @ Sat, 15 Jan 2022 19:35:36: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:35:36: #1 finished! INFO @ Sat, 15 Jan 2022 19:35:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:35:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:35:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:35:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:35:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:35:41: 16000000 INFO @ Sat, 15 Jan 2022 19:35:41: 13000000 INFO @ Sat, 15 Jan 2022 19:35:50: 17000000 INFO @ Sat, 15 Jan 2022 19:35:50: 14000000 INFO @ Sat, 15 Jan 2022 19:35:59: 18000000 INFO @ Sat, 15 Jan 2022 19:35:59: 15000000 INFO @ Sat, 15 Jan 2022 19:36:05: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:36:05: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:36:05: #1 total tags in treatment: 8890961 INFO @ Sat, 15 Jan 2022 19:36:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:05: #1 tags after filtering in treatment: 5210675 INFO @ Sat, 15 Jan 2022 19:36:05: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:36:05: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:36:08: 16000000 INFO @ Sat, 15 Jan 2022 19:36:16: 17000000 INFO @ Sat, 15 Jan 2022 19:36:25: 18000000 INFO @ Sat, 15 Jan 2022 19:36:32: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:36:32: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:36:32: #1 total tags in treatment: 8890961 INFO @ Sat, 15 Jan 2022 19:36:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:32: #1 tags after filtering in treatment: 5210675 INFO @ Sat, 15 Jan 2022 19:36:32: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:36:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659049/SRX3659049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling