Job ID = 14520446 SRX = SRX3659046 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9855844 spots for SRR6682743/SRR6682743.sra Written 9855844 spots for SRR6682743/SRR6682743.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:33 9855844 reads; of these: 9855844 (100.00%) were paired; of these: 909582 (9.23%) aligned concordantly 0 times 8101530 (82.20%) aligned concordantly exactly 1 time 844732 (8.57%) aligned concordantly >1 times ---- 909582 pairs aligned concordantly 0 times; of these: 566403 (62.27%) aligned discordantly 1 time ---- 343179 pairs aligned 0 times concordantly or discordantly; of these: 686358 mates make up the pairs; of these: 466199 (67.92%) aligned 0 times 91646 (13.35%) aligned exactly 1 time 128513 (18.72%) aligned >1 times 97.63% overall alignment rate Time searching: 00:14:33 Overall time: 00:14:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 867685 / 9435309 = 0.0920 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:33:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:33:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:33:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:33:57: 1000000 INFO @ Sat, 15 Jan 2022 19:34:09: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:20: 3000000 INFO @ Sat, 15 Jan 2022 19:34:28: 1000000 INFO @ Sat, 15 Jan 2022 19:34:31: 4000000 INFO @ Sat, 15 Jan 2022 19:34:38: 2000000 INFO @ Sat, 15 Jan 2022 19:34:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:34:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:34:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:34:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:34:49: 3000000 INFO @ Sat, 15 Jan 2022 19:34:51: 6000000 INFO @ Sat, 15 Jan 2022 19:34:57: 1000000 INFO @ Sat, 15 Jan 2022 19:35:00: 4000000 INFO @ Sat, 15 Jan 2022 19:35:02: 7000000 INFO @ Sat, 15 Jan 2022 19:35:07: 2000000 INFO @ Sat, 15 Jan 2022 19:35:12: 5000000 INFO @ Sat, 15 Jan 2022 19:35:13: 8000000 INFO @ Sat, 15 Jan 2022 19:35:16: 3000000 INFO @ Sat, 15 Jan 2022 19:35:23: 6000000 INFO @ Sat, 15 Jan 2022 19:35:23: 9000000 INFO @ Sat, 15 Jan 2022 19:35:25: 4000000 INFO @ Sat, 15 Jan 2022 19:35:33: 7000000 INFO @ Sat, 15 Jan 2022 19:35:33: 10000000 INFO @ Sat, 15 Jan 2022 19:35:34: 5000000 INFO @ Sat, 15 Jan 2022 19:35:43: 11000000 INFO @ Sat, 15 Jan 2022 19:35:43: 6000000 INFO @ Sat, 15 Jan 2022 19:35:44: 8000000 INFO @ Sat, 15 Jan 2022 19:35:52: 7000000 INFO @ Sat, 15 Jan 2022 19:35:53: 12000000 INFO @ Sat, 15 Jan 2022 19:35:55: 9000000 INFO @ Sat, 15 Jan 2022 19:36:00: 8000000 INFO @ Sat, 15 Jan 2022 19:36:03: 13000000 INFO @ Sat, 15 Jan 2022 19:36:05: 10000000 INFO @ Sat, 15 Jan 2022 19:36:09: 9000000 INFO @ Sat, 15 Jan 2022 19:36:13: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:36:16: 11000000 INFO @ Sat, 15 Jan 2022 19:36:18: 10000000 INFO @ Sat, 15 Jan 2022 19:36:23: 15000000 INFO @ Sat, 15 Jan 2022 19:36:26: 11000000 INFO @ Sat, 15 Jan 2022 19:36:27: 12000000 INFO @ Sat, 15 Jan 2022 19:36:34: 16000000 INFO @ Sat, 15 Jan 2022 19:36:35: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:36:37: 13000000 INFO @ Sat, 15 Jan 2022 19:36:43: 13000000 INFO @ Sat, 15 Jan 2022 19:36:45: 17000000 INFO @ Sat, 15 Jan 2022 19:36:47: 14000000 INFO @ Sat, 15 Jan 2022 19:36:51: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:36:51: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:36:51: #1 total tags in treatment: 8106872 INFO @ Sat, 15 Jan 2022 19:36:51: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:51: #1 tags after filtering in treatment: 4984278 INFO @ Sat, 15 Jan 2022 19:36:51: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:36:51: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:51: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:36:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:36:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:36:52: 14000000 INFO @ Sat, 15 Jan 2022 19:36:57: 15000000 INFO @ Sat, 15 Jan 2022 19:37:01: 15000000 INFO @ Sat, 15 Jan 2022 19:37:07: 16000000 INFO @ Sat, 15 Jan 2022 19:37:10: 16000000 INFO @ Sat, 15 Jan 2022 19:37:18: 17000000 INFO @ Sat, 15 Jan 2022 19:37:18: 17000000 INFO @ Sat, 15 Jan 2022 19:37:23: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:37:23: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:37:23: #1 total tags in treatment: 8106872 INFO @ Sat, 15 Jan 2022 19:37:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:37:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:37:23: #1 tags after filtering in treatment: 4984278 INFO @ Sat, 15 Jan 2022 19:37:23: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:37:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:37:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:37:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:37:24: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:37:24: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:37:24: #1 total tags in treatment: 8106872 INFO @ Sat, 15 Jan 2022 19:37:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:37:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:37:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:37:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:37:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 15 Jan 2022 19:37:24: #1 tags after filtering in treatment: 4984278 INFO @ Sat, 15 Jan 2022 19:37:24: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:37:24: #1 finished! INFO @ Sat, 15 Jan 2022 19:37:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:37:24: #2 looking for paired plus/minus strand peaks... needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:37:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:37:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:37:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3659046/SRX3659046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling