Job ID = 2640841 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,263,444 reads read : 19,263,444 reads written : 19,263,444 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1009217.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 19263444 reads; of these: 19263444 (100.00%) were unpaired; of these: 3999303 (20.76%) aligned 0 times 12662589 (65.73%) aligned exactly 1 time 2601552 (13.51%) aligned >1 times 79.24% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5941427 / 15264141 = 0.3892 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:41:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:41:02: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:41:02: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:41:12: 1000000 INFO @ Sat, 24 Aug 2019 19:41:22: 2000000 INFO @ Sat, 24 Aug 2019 19:41:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:41:31: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:41:31: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:41:32: 3000000 INFO @ Sat, 24 Aug 2019 19:41:40: 1000000 INFO @ Sat, 24 Aug 2019 19:41:41: 4000000 INFO @ Sat, 24 Aug 2019 19:41:49: 2000000 INFO @ Sat, 24 Aug 2019 19:41:51: 5000000 INFO @ Sat, 24 Aug 2019 19:41:57: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:42:00: 6000000 INFO @ Sat, 24 Aug 2019 19:42:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:42:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:42:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:42:06: 4000000 INFO @ Sat, 24 Aug 2019 19:42:10: 1000000 INFO @ Sat, 24 Aug 2019 19:42:10: 7000000 INFO @ Sat, 24 Aug 2019 19:42:15: 5000000 INFO @ Sat, 24 Aug 2019 19:42:19: 2000000 INFO @ Sat, 24 Aug 2019 19:42:20: 8000000 INFO @ Sat, 24 Aug 2019 19:42:23: 6000000 INFO @ Sat, 24 Aug 2019 19:42:27: 3000000 INFO @ Sat, 24 Aug 2019 19:42:29: 9000000 INFO @ Sat, 24 Aug 2019 19:42:32: 7000000 INFO @ Sat, 24 Aug 2019 19:42:32: #1 tag size is determined as 100 bps INFO @ Sat, 24 Aug 2019 19:42:32: #1 tag size = 100 INFO @ Sat, 24 Aug 2019 19:42:32: #1 total tags in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:42:32: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:42:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:42:33: #1 tags after filtering in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:42:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:42:33: #1 finished! INFO @ Sat, 24 Aug 2019 19:42:33: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:42:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:42:33: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:42:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:42:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:42:36: 4000000 INFO @ Sat, 24 Aug 2019 19:42:41: 8000000 INFO @ Sat, 24 Aug 2019 19:42:45: 5000000 INFO @ Sat, 24 Aug 2019 19:42:49: 9000000 INFO @ Sat, 24 Aug 2019 19:42:52: #1 tag size is determined as 100 bps INFO @ Sat, 24 Aug 2019 19:42:52: #1 tag size = 100 INFO @ Sat, 24 Aug 2019 19:42:52: #1 total tags in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:42:52: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:42:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:42:52: #1 tags after filtering in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:42:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:42:52: #1 finished! INFO @ Sat, 24 Aug 2019 19:42:52: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:42:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:42:53: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:42:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:42:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:42:53: 6000000 INFO @ Sat, 24 Aug 2019 19:43:02: 7000000 INFO @ Sat, 24 Aug 2019 19:43:10: 8000000 INFO @ Sat, 24 Aug 2019 19:43:19: 9000000 INFO @ Sat, 24 Aug 2019 19:43:21: #1 tag size is determined as 100 bps INFO @ Sat, 24 Aug 2019 19:43:21: #1 tag size = 100 INFO @ Sat, 24 Aug 2019 19:43:21: #1 total tags in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:43:21: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:43:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:43:21: #1 tags after filtering in treatment: 9322714 INFO @ Sat, 24 Aug 2019 19:43:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:43:21: #1 finished! INFO @ Sat, 24 Aug 2019 19:43:21: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:43:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:43:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:43:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:43:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363335/SRX363335.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。