Job ID = 2010318 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-07-05T13:07:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T13:09:16 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T13:12:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 13,808,287 reads read : 27,616,574 reads written : 27,616,574 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:00 13808287 reads; of these: 13808287 (100.00%) were paired; of these: 4363954 (31.60%) aligned concordantly 0 times 5444424 (39.43%) aligned concordantly exactly 1 time 3999909 (28.97%) aligned concordantly >1 times ---- 4363954 pairs aligned concordantly 0 times; of these: 3714 (0.09%) aligned discordantly 1 time ---- 4360240 pairs aligned 0 times concordantly or discordantly; of these: 8720480 mates make up the pairs; of these: 8036484 (92.16%) aligned 0 times 178151 (2.04%) aligned exactly 1 time 505845 (5.80%) aligned >1 times 70.90% overall alignment rate Time searching: 00:11:00 Overall time: 00:11:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2312728 / 9439455 = 0.2450 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:39:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:39:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:39:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:39:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:39:25: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:39:25: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:39:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:39:26: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:39:26: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:39:35: 1000000 INFO @ Fri, 05 Jul 2019 22:39:36: 1000000 INFO @ Fri, 05 Jul 2019 22:39:37: 1000000 INFO @ Fri, 05 Jul 2019 22:39:46: 2000000 INFO @ Fri, 05 Jul 2019 22:39:48: 2000000 INFO @ Fri, 05 Jul 2019 22:39:49: 2000000 INFO @ Fri, 05 Jul 2019 22:39:59: 3000000 INFO @ Fri, 05 Jul 2019 22:40:01: 3000000 INFO @ Fri, 05 Jul 2019 22:40:02: 3000000 INFO @ Fri, 05 Jul 2019 22:40:12: 4000000 INFO @ Fri, 05 Jul 2019 22:40:13: 4000000 INFO @ Fri, 05 Jul 2019 22:40:15: 4000000 INFO @ Fri, 05 Jul 2019 22:40:24: 5000000 INFO @ Fri, 05 Jul 2019 22:40:26: 5000000 INFO @ Fri, 05 Jul 2019 22:40:27: 5000000 INFO @ Fri, 05 Jul 2019 22:40:36: 6000000 INFO @ Fri, 05 Jul 2019 22:40:37: 6000000 INFO @ Fri, 05 Jul 2019 22:40:39: 6000000 INFO @ Fri, 05 Jul 2019 22:40:45: 7000000 INFO @ Fri, 05 Jul 2019 22:40:47: 7000000 INFO @ Fri, 05 Jul 2019 22:40:48: 7000000 INFO @ Fri, 05 Jul 2019 22:40:53: 8000000 INFO @ Fri, 05 Jul 2019 22:40:56: 8000000 INFO @ Fri, 05 Jul 2019 22:40:57: 8000000 INFO @ Fri, 05 Jul 2019 22:41:00: 9000000 INFO @ Fri, 05 Jul 2019 22:41:05: 9000000 INFO @ Fri, 05 Jul 2019 22:41:06: 9000000 INFO @ Fri, 05 Jul 2019 22:41:08: 10000000 INFO @ Fri, 05 Jul 2019 22:41:14: 10000000 INFO @ Fri, 05 Jul 2019 22:41:15: 10000000 INFO @ Fri, 05 Jul 2019 22:41:16: 11000000 INFO @ Fri, 05 Jul 2019 22:41:23: 11000000 INFO @ Fri, 05 Jul 2019 22:41:23: 12000000 INFO @ Fri, 05 Jul 2019 22:41:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 22:41:31: 13000000 INFO @ Fri, 05 Jul 2019 22:41:32: 12000000 INFO @ Fri, 05 Jul 2019 22:41:33: 12000000 INFO @ Fri, 05 Jul 2019 22:41:39: 14000000 INFO @ Fri, 05 Jul 2019 22:41:41: 13000000 INFO @ Fri, 05 Jul 2019 22:41:42: 13000000 INFO @ Fri, 05 Jul 2019 22:41:46: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 22:41:46: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 22:41:46: #1 total tags in treatment: 7132037 INFO @ Fri, 05 Jul 2019 22:41:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:46: #1 tags after filtering in treatment: 4303524 INFO @ Fri, 05 Jul 2019 22:41:46: #1 Redundant rate of treatment: 0.40 INFO @ Fri, 05 Jul 2019 22:41:46: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:46: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:41:47: #2 number of paired peaks: 44 WARNING @ Fri, 05 Jul 2019 22:41:47: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:41:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:41:50: 14000000 INFO @ Fri, 05 Jul 2019 22:41:51: 14000000 BigWig に変換しました。 INFO @ Fri, 05 Jul 2019 22:41:58: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 22:41:58: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 22:41:58: #1 total tags in treatment: 7132037 INFO @ Fri, 05 Jul 2019 22:41:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:58: #1 tags after filtering in treatment: 4303524 INFO @ Fri, 05 Jul 2019 22:41:58: #1 Redundant rate of treatment: 0.40 INFO @ Fri, 05 Jul 2019 22:41:58: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:41:59: #2 number of paired peaks: 44 WARNING @ Fri, 05 Jul 2019 22:41:59: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:41:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 179 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:41:59: #1 tag size is determined as 45 bps INFO @ Fri, 05 Jul 2019 22:41:59: #1 tag size = 45 INFO @ Fri, 05 Jul 2019 22:41:59: #1 total tags in treatment: 7132037 INFO @ Fri, 05 Jul 2019 22:41:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:59: #1 tags after filtering in treatment: 4303524 INFO @ Fri, 05 Jul 2019 22:41:59: #1 Redundant rate of treatment: 0.40 INFO @ Fri, 05 Jul 2019 22:41:59: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:42:00: #2 number of paired peaks: 44 WARNING @ Fri, 05 Jul 2019 22:42:00: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:42:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363259/SRX363259.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling