Job ID = 11162617 sra ファイルのダウンロード中... Completed: 518661K bytes transferred in 47 seconds (88719K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 16432184 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585222/SRR6495922.sra Written 16432184 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585222/SRR6495922.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 16432184 reads; of these: 16432184 (100.00%) were unpaired; of these: 1825711 (11.11%) aligned 0 times 12774879 (77.74%) aligned exactly 1 time 1831594 (11.15%) aligned >1 times 88.89% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5546972 / 14606473 = 0.3798 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:37:54: # Command line: callpeak -t SRX3585222.bam -f BAM -g 12100000 -n SRX3585222.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3585222.05 # format = BAM # ChIP-seq file = ['SRX3585222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:54: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:54: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:37:54: # Command line: callpeak -t SRX3585222.bam -f BAM -g 12100000 -n SRX3585222.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3585222.20 # format = BAM # ChIP-seq file = ['SRX3585222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:54: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:54: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:37:54: # Command line: callpeak -t SRX3585222.bam -f BAM -g 12100000 -n SRX3585222.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3585222.10 # format = BAM # ChIP-seq file = ['SRX3585222.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:54: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:54: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:38:01: 1000000 INFO @ Wed, 05 Sep 2018 10:38:02: 1000000 INFO @ Wed, 05 Sep 2018 10:38:02: 1000000 INFO @ Wed, 05 Sep 2018 10:38:08: 2000000 INFO @ Wed, 05 Sep 2018 10:38:09: 2000000 INFO @ Wed, 05 Sep 2018 10:38:09: 2000000 INFO @ Wed, 05 Sep 2018 10:38:15: 3000000 INFO @ Wed, 05 Sep 2018 10:38:16: 3000000 INFO @ Wed, 05 Sep 2018 10:38:16: 3000000 INFO @ Wed, 05 Sep 2018 10:38:22: 4000000 INFO @ Wed, 05 Sep 2018 10:38:23: 4000000 INFO @ Wed, 05 Sep 2018 10:38:23: 4000000 INFO @ Wed, 05 Sep 2018 10:38:29: 5000000 INFO @ Wed, 05 Sep 2018 10:38:30: 5000000 INFO @ Wed, 05 Sep 2018 10:38:30: 5000000 INFO @ Wed, 05 Sep 2018 10:38:36: 6000000 INFO @ Wed, 05 Sep 2018 10:38:37: 6000000 INFO @ Wed, 05 Sep 2018 10:38:37: 6000000 INFO @ Wed, 05 Sep 2018 10:38:43: 7000000 INFO @ Wed, 05 Sep 2018 10:38:44: 7000000 INFO @ Wed, 05 Sep 2018 10:38:44: 7000000 INFO @ Wed, 05 Sep 2018 10:38:50: 8000000 INFO @ Wed, 05 Sep 2018 10:38:52: 8000000 INFO @ Wed, 05 Sep 2018 10:38:52: 8000000 INFO @ Wed, 05 Sep 2018 10:38:57: 9000000 INFO @ Wed, 05 Sep 2018 10:38:57: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:57: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:57: #1 total tags in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:57: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:57: #1 tags after filtering in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:57: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:57: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:38:58: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:38:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:38:58: Process for pairing-model is terminated! cat: SRX3585222.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585222.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:38:59: 9000000 INFO @ Wed, 05 Sep 2018 10:38:59: 9000000 INFO @ Wed, 05 Sep 2018 10:38:59: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:59: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:59: #1 total tags in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:59: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:59: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:59: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:59: #1 total tags in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:59: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:59: #1 tags after filtering in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:59: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:59: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:38:59: #1 tags after filtering in treatment: 9059501 INFO @ Wed, 05 Sep 2018 10:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:59: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:59: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:39:00: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:39:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:39:00: Process for pairing-model is terminated! cat: SRX3585222.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis INFO @ Wed, 05 Sep 2018 10:39:00: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:39:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:39:00: Process for pairing-model is terminated! cat: SRX3585222.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585222.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585222.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585222.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。