Job ID = 11162600 sra ファイルのダウンロード中... Completed: 909648K bytes transferred in 10 seconds (731255K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 49611249 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585210/SRR6495910.sra Written 49611249 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585210/SRR6495910.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:29 49611249 reads; of these: 49611249 (100.00%) were unpaired; of these: 13635781 (27.49%) aligned 0 times 32584131 (65.68%) aligned exactly 1 time 3391337 (6.84%) aligned >1 times 72.51% overall alignment rate Time searching: 00:07:29 Overall time: 00:07:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 22849835 / 35975468 = 0.6352 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:37:14: # Command line: callpeak -t SRX3585210.bam -f BAM -g 12100000 -n SRX3585210.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3585210.20 # format = BAM # ChIP-seq file = ['SRX3585210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:14: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:14: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:37:14: # Command line: callpeak -t SRX3585210.bam -f BAM -g 12100000 -n SRX3585210.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3585210.10 # format = BAM # ChIP-seq file = ['SRX3585210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:14: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:14: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:37:14: # Command line: callpeak -t SRX3585210.bam -f BAM -g 12100000 -n SRX3585210.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3585210.05 # format = BAM # ChIP-seq file = ['SRX3585210.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:37:14: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:37:14: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:37:21: 1000000 INFO @ Wed, 05 Sep 2018 10:37:22: 1000000 INFO @ Wed, 05 Sep 2018 10:37:22: 1000000 INFO @ Wed, 05 Sep 2018 10:37:28: 2000000 INFO @ Wed, 05 Sep 2018 10:37:29: 2000000 INFO @ Wed, 05 Sep 2018 10:37:29: 2000000 INFO @ Wed, 05 Sep 2018 10:37:35: 3000000 INFO @ Wed, 05 Sep 2018 10:37:37: 3000000 INFO @ Wed, 05 Sep 2018 10:37:37: 3000000 INFO @ Wed, 05 Sep 2018 10:37:42: 4000000 INFO @ Wed, 05 Sep 2018 10:37:45: 4000000 INFO @ Wed, 05 Sep 2018 10:37:45: 4000000 INFO @ Wed, 05 Sep 2018 10:37:48: 5000000 INFO @ Wed, 05 Sep 2018 10:37:52: 5000000 INFO @ Wed, 05 Sep 2018 10:37:52: 5000000 INFO @ Wed, 05 Sep 2018 10:37:55: 6000000 INFO @ Wed, 05 Sep 2018 10:38:00: 6000000 INFO @ Wed, 05 Sep 2018 10:38:00: 6000000 INFO @ Wed, 05 Sep 2018 10:38:02: 7000000 INFO @ Wed, 05 Sep 2018 10:38:08: 7000000 INFO @ Wed, 05 Sep 2018 10:38:08: 7000000 INFO @ Wed, 05 Sep 2018 10:38:09: 8000000 INFO @ Wed, 05 Sep 2018 10:38:16: 8000000 INFO @ Wed, 05 Sep 2018 10:38:16: 8000000 INFO @ Wed, 05 Sep 2018 10:38:16: 9000000 INFO @ Wed, 05 Sep 2018 10:38:22: 10000000 INFO @ Wed, 05 Sep 2018 10:38:23: 9000000 INFO @ Wed, 05 Sep 2018 10:38:23: 9000000 INFO @ Wed, 05 Sep 2018 10:38:29: 11000000 INFO @ Wed, 05 Sep 2018 10:38:31: 10000000 INFO @ Wed, 05 Sep 2018 10:38:31: 10000000 INFO @ Wed, 05 Sep 2018 10:38:36: 12000000 INFO @ Wed, 05 Sep 2018 10:38:39: 11000000 INFO @ Wed, 05 Sep 2018 10:38:39: 11000000 INFO @ Wed, 05 Sep 2018 10:38:42: 13000000 INFO @ Wed, 05 Sep 2018 10:38:43: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:43: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:43: #1 total tags in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:43: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:44: #1 tags after filtering in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:44: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:44: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:38:44: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:38:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:38:44: Process for pairing-model is terminated! cat: SRX3585210.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585210.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:38:46: 12000000 INFO @ Wed, 05 Sep 2018 10:38:46: 12000000 INFO @ Wed, 05 Sep 2018 10:38:53: 13000000 INFO @ Wed, 05 Sep 2018 10:38:53: 13000000 INFO @ Wed, 05 Sep 2018 10:38:54: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:54: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:54: #1 total tags in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:54: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:54: #1 tags after filtering in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:54: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:54: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:38:54: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:38:54: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:38:54: #1 total tags in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:54: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:38:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:38:54: #1 tags after filtering in treatment: 13125633 INFO @ Wed, 05 Sep 2018 10:38:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:38:54: #1 finished! INFO @ Wed, 05 Sep 2018 10:38:54: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:38:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:38:55: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:38:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:38:55: Process for pairing-model is terminated! cat: SRX3585210.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585210.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:38:55: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:38:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:38:55: Process for pairing-model is terminated! cat: SRX3585210.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585210.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585210.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。