Job ID = 11162595 sra ファイルのダウンロード中... Completed: 976055K bytes transferred in 10 seconds (739215K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 53582696 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585205/SRR6495905.sra Written 53582696 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585205/SRR6495905.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:28 53582696 reads; of these: 53582696 (100.00%) were unpaired; of these: 11500985 (21.46%) aligned 0 times 37817766 (70.58%) aligned exactly 1 time 4263945 (7.96%) aligned >1 times 78.54% overall alignment rate Time searching: 00:09:28 Overall time: 00:09:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28325990 / 42081711 = 0.6731 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:38:22: # Command line: callpeak -t SRX3585205.bam -f BAM -g 12100000 -n SRX3585205.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3585205.05 # format = BAM # ChIP-seq file = ['SRX3585205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:38:22: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:38:22: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:38:22: # Command line: callpeak -t SRX3585205.bam -f BAM -g 12100000 -n SRX3585205.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3585205.20 # format = BAM # ChIP-seq file = ['SRX3585205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:38:22: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:38:22: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:38:22: # Command line: callpeak -t SRX3585205.bam -f BAM -g 12100000 -n SRX3585205.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3585205.10 # format = BAM # ChIP-seq file = ['SRX3585205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:38:22: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:38:22: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:38:28: 1000000 INFO @ Wed, 05 Sep 2018 10:38:29: 1000000 INFO @ Wed, 05 Sep 2018 10:38:29: 1000000 INFO @ Wed, 05 Sep 2018 10:38:35: 2000000 INFO @ Wed, 05 Sep 2018 10:38:37: 2000000 INFO @ Wed, 05 Sep 2018 10:38:37: 2000000 INFO @ Wed, 05 Sep 2018 10:38:42: 3000000 INFO @ Wed, 05 Sep 2018 10:38:45: 3000000 INFO @ Wed, 05 Sep 2018 10:38:45: 3000000 INFO @ Wed, 05 Sep 2018 10:38:49: 4000000 INFO @ Wed, 05 Sep 2018 10:38:54: 4000000 INFO @ Wed, 05 Sep 2018 10:38:54: 4000000 INFO @ Wed, 05 Sep 2018 10:38:57: 5000000 INFO @ Wed, 05 Sep 2018 10:39:04: 5000000 INFO @ Wed, 05 Sep 2018 10:39:04: 5000000 INFO @ Wed, 05 Sep 2018 10:39:06: 6000000 INFO @ Wed, 05 Sep 2018 10:39:14: 6000000 INFO @ Wed, 05 Sep 2018 10:39:14: 6000000 INFO @ Wed, 05 Sep 2018 10:39:14: 7000000 INFO @ Wed, 05 Sep 2018 10:39:21: 8000000 INFO @ Wed, 05 Sep 2018 10:39:23: 7000000 INFO @ Wed, 05 Sep 2018 10:39:23: 7000000 INFO @ Wed, 05 Sep 2018 10:39:29: 9000000 INFO @ Wed, 05 Sep 2018 10:39:32: 8000000 INFO @ Wed, 05 Sep 2018 10:39:32: 8000000 INFO @ Wed, 05 Sep 2018 10:39:36: 10000000 INFO @ Wed, 05 Sep 2018 10:39:41: 9000000 INFO @ Wed, 05 Sep 2018 10:39:41: 9000000 INFO @ Wed, 05 Sep 2018 10:39:44: 11000000 INFO @ Wed, 05 Sep 2018 10:39:51: 10000000 INFO @ Wed, 05 Sep 2018 10:39:51: 10000000 INFO @ Wed, 05 Sep 2018 10:39:51: 12000000 INFO @ Wed, 05 Sep 2018 10:39:59: 13000000 INFO @ Wed, 05 Sep 2018 10:40:00: 11000000 INFO @ Wed, 05 Sep 2018 10:40:00: 11000000 INFO @ Wed, 05 Sep 2018 10:40:05: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:40:05: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:40:05: #1 total tags in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:05: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:40:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:40:05: #1 tags after filtering in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:40:05: #1 finished! INFO @ Wed, 05 Sep 2018 10:40:05: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:40:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:40:06: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:40:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:40:06: Process for pairing-model is terminated! cat: SRX3585205.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585205.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585205.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585205.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:40:09: 12000000 INFO @ Wed, 05 Sep 2018 10:40:09: 12000000 INFO @ Wed, 05 Sep 2018 10:40:18: 13000000 INFO @ Wed, 05 Sep 2018 10:40:18: 13000000 INFO @ Wed, 05 Sep 2018 10:40:25: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:40:25: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:40:25: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:40:25: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:40:25: #1 total tags in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:25: #1 total tags in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:25: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:40:25: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:40:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:40:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:40:25: #1 tags after filtering in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:25: #1 tags after filtering in treatment: 13755721 INFO @ Wed, 05 Sep 2018 10:40:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:40:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:40:25: #1 finished! INFO @ Wed, 05 Sep 2018 10:40:25: #1 finished! INFO @ Wed, 05 Sep 2018 10:40:25: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:40:25: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:40:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:40:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:40:26: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:40:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:40:26: Process for pairing-model is terminated! INFO @ Wed, 05 Sep 2018 10:40:26: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:40:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:40:26: Process for pairing-model is terminated! cat: SRX3585205.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3585205.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX3585205.10_model.r': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585205.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585205.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX3585205.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585205.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585205.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。