Job ID = 11162587 sra ファイルのダウンロード中... Completed: 735881K bytes transferred in 9 seconds (669496K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 23779098 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585198/SRR6495898.sra Written 23779098 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3585198/SRR6495898.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:31 23779098 reads; of these: 23779098 (100.00%) were unpaired; of these: 7664638 (32.23%) aligned 0 times 14182530 (59.64%) aligned exactly 1 time 1931930 (8.12%) aligned >1 times 67.77% overall alignment rate Time searching: 00:03:31 Overall time: 00:03:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8498164 / 16114460 = 0.5274 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 05 Sep 2018 10:18:01: # Command line: callpeak -t SRX3585198.bam -f BAM -g 12100000 -n SRX3585198.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3585198.10 # format = BAM # ChIP-seq file = ['SRX3585198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:18:01: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:18:01: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:18:01: # Command line: callpeak -t SRX3585198.bam -f BAM -g 12100000 -n SRX3585198.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3585198.20 # format = BAM # ChIP-seq file = ['SRX3585198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:18:01: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:18:01: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:18:01: # Command line: callpeak -t SRX3585198.bam -f BAM -g 12100000 -n SRX3585198.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3585198.05 # format = BAM # ChIP-seq file = ['SRX3585198.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 05 Sep 2018 10:18:01: #1 read tag files... INFO @ Wed, 05 Sep 2018 10:18:01: #1 read treatment tags... INFO @ Wed, 05 Sep 2018 10:18:07: 1000000 INFO @ Wed, 05 Sep 2018 10:18:07: 1000000 INFO @ Wed, 05 Sep 2018 10:18:07: 1000000 INFO @ Wed, 05 Sep 2018 10:18:13: 2000000 INFO @ Wed, 05 Sep 2018 10:18:14: 2000000 INFO @ Wed, 05 Sep 2018 10:18:14: 2000000 INFO @ Wed, 05 Sep 2018 10:18:19: 3000000 INFO @ Wed, 05 Sep 2018 10:18:20: 3000000 INFO @ Wed, 05 Sep 2018 10:18:20: 3000000 INFO @ Wed, 05 Sep 2018 10:18:25: 4000000 INFO @ Wed, 05 Sep 2018 10:18:26: 4000000 INFO @ Wed, 05 Sep 2018 10:18:26: 4000000 INFO @ Wed, 05 Sep 2018 10:18:31: 5000000 INFO @ Wed, 05 Sep 2018 10:18:33: 5000000 INFO @ Wed, 05 Sep 2018 10:18:33: 5000000 INFO @ Wed, 05 Sep 2018 10:18:37: 6000000 INFO @ Wed, 05 Sep 2018 10:18:39: 6000000 INFO @ Wed, 05 Sep 2018 10:18:39: 6000000 INFO @ Wed, 05 Sep 2018 10:18:44: 7000000 INFO @ Wed, 05 Sep 2018 10:18:45: 7000000 INFO @ Wed, 05 Sep 2018 10:18:45: 7000000 INFO @ Wed, 05 Sep 2018 10:18:47: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:18:47: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:18:47: #1 total tags in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:47: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:18:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:18:47: #1 tags after filtering in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:47: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:18:47: #1 finished! INFO @ Wed, 05 Sep 2018 10:18:47: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:18:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:18:48: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:18:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:18:48: Process for pairing-model is terminated! cat: SRX3585198.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585198.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585198.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585198.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 05 Sep 2018 10:18:49: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:18:49: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:18:49: #1 total tags in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:49: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:18:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:18:49: #1 tag size is determined as 50 bps INFO @ Wed, 05 Sep 2018 10:18:49: #1 tag size = 50 INFO @ Wed, 05 Sep 2018 10:18:49: #1 total tags in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:49: #1 user defined the maximum tags... INFO @ Wed, 05 Sep 2018 10:18:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 05 Sep 2018 10:18:49: #1 tags after filtering in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:18:49: #1 finished! INFO @ Wed, 05 Sep 2018 10:18:49: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:18:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:18:49: #1 tags after filtering in treatment: 7616296 INFO @ Wed, 05 Sep 2018 10:18:49: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 05 Sep 2018 10:18:49: #1 finished! INFO @ Wed, 05 Sep 2018 10:18:49: #2 Build Peak Model... INFO @ Wed, 05 Sep 2018 10:18:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 05 Sep 2018 10:18:50: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:18:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:18:50: Process for pairing-model is terminated! INFO @ Wed, 05 Sep 2018 10:18:50: #2 number of paired peaks: 0 WARNING @ Wed, 05 Sep 2018 10:18:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 05 Sep 2018 10:18:50: Process for pairing-model is terminated! cat: SRX3585198.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3585198.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 5 millis rm: cannot remove `SRX3585198.05_model.r': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3585198.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585198.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX3585198.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585198.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3585198.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。