Job ID = 11192957 sra ファイルのダウンロード中... Completed: 260889K bytes transferred in 10 seconds (195178K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 7810853 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3544075/SRR6453181.sra Written 7810853 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3544075/SRR6453181.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:33 7810853 reads; of these: 7810853 (100.00%) were paired; of these: 2738273 (35.06%) aligned concordantly 0 times 3404029 (43.58%) aligned concordantly exactly 1 time 1668551 (21.36%) aligned concordantly >1 times ---- 2738273 pairs aligned concordantly 0 times; of these: 214553 (7.84%) aligned discordantly 1 time ---- 2523720 pairs aligned 0 times concordantly or discordantly; of these: 5047440 mates make up the pairs; of these: 3427004 (67.90%) aligned 0 times 159065 (3.15%) aligned exactly 1 time 1461371 (28.95%) aligned >1 times 78.06% overall alignment rate Time searching: 00:04:33 Overall time: 00:04:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2713228 / 5257617 = 0.5161 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:44:50: # Command line: callpeak -t SRX3544075.bam -f BAM -g 12100000 -n SRX3544075.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3544075.20 # format = BAM # ChIP-seq file = ['SRX3544075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:44:50: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:44:50: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:44:50: # Command line: callpeak -t SRX3544075.bam -f BAM -g 12100000 -n SRX3544075.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3544075.05 # format = BAM # ChIP-seq file = ['SRX3544075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:44:50: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:44:50: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:44:50: # Command line: callpeak -t SRX3544075.bam -f BAM -g 12100000 -n SRX3544075.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3544075.10 # format = BAM # ChIP-seq file = ['SRX3544075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:44:50: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:44:50: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:44:56: 1000000 INFO @ Sat, 15 Sep 2018 10:44:56: 1000000 INFO @ Sat, 15 Sep 2018 10:44:56: 1000000 INFO @ Sat, 15 Sep 2018 10:45:01: 2000000 INFO @ Sat, 15 Sep 2018 10:45:01: 2000000 INFO @ Sat, 15 Sep 2018 10:45:02: 2000000 INFO @ Sat, 15 Sep 2018 10:45:07: 3000000 INFO @ Sat, 15 Sep 2018 10:45:07: 3000000 INFO @ Sat, 15 Sep 2018 10:45:07: 3000000 INFO @ Sat, 15 Sep 2018 10:45:12: 4000000 INFO @ Sat, 15 Sep 2018 10:45:12: 4000000 INFO @ Sat, 15 Sep 2018 10:45:12: 4000000 INFO @ Sat, 15 Sep 2018 10:45:17: 5000000 INFO @ Sat, 15 Sep 2018 10:45:17: 5000000 INFO @ Sat, 15 Sep 2018 10:45:17: 5000000 INFO @ Sat, 15 Sep 2018 10:45:23: 6000000 INFO @ Sat, 15 Sep 2018 10:45:23: 6000000 INFO @ Sat, 15 Sep 2018 10:45:23: 6000000 INFO @ Sat, 15 Sep 2018 10:45:27: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:45:27: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:45:27: #1 total tags in treatment: 2499211 INFO @ Sat, 15 Sep 2018 10:45:27: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:45:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:45:27: #1 tags after filtering in treatment: 1710587 INFO @ Sat, 15 Sep 2018 10:45:27: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Sep 2018 10:45:27: #1 finished! INFO @ Sat, 15 Sep 2018 10:45:27: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:45:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:45:27: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:45:27: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:45:27: #1 total tags in treatment: 2499211 INFO @ Sat, 15 Sep 2018 10:45:27: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:45:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:45:27: #1 tags after filtering in treatment: 1710587 INFO @ Sat, 15 Sep 2018 10:45:27: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Sep 2018 10:45:27: #1 finished! INFO @ Sat, 15 Sep 2018 10:45:27: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:45:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:45:28: #2 number of paired peaks: 32 WARNING @ Sat, 15 Sep 2018 10:45:28: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:45:28: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:45:28: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:45:28: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:45:28: #1 total tags in treatment: 2499211 INFO @ Sat, 15 Sep 2018 10:45:28: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cat: SRX3544075.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544075.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:45:28: #1 tags after filtering in treatment: 1710587 INFO @ Sat, 15 Sep 2018 10:45:28: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Sep 2018 10:45:28: #1 finished! INFO @ Sat, 15 Sep 2018 10:45:28: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:45:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:45:28: #2 number of paired peaks: 32 WARNING @ Sat, 15 Sep 2018 10:45:28: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:45:28: Process for pairing-model is terminated! cat: SRX3544075.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544075.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:45:28: #2 number of paired peaks: 32 WARNING @ Sat, 15 Sep 2018 10:45:28: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:45:28: Process for pairing-model is terminated! cat: SRX3544075.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544075.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544075.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。