Job ID = 11192956 sra ファイルのダウンロード中... Completed: 309222K bytes transferred in 8 seconds (285883K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 9231485 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3544074/SRR6453180.sra Written 9231485 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3544074/SRR6453180.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:39 9231485 reads; of these: 9231485 (100.00%) were paired; of these: 1314163 (14.24%) aligned concordantly 0 times 4558796 (49.38%) aligned concordantly exactly 1 time 3358526 (36.38%) aligned concordantly >1 times ---- 1314163 pairs aligned concordantly 0 times; of these: 121677 (9.26%) aligned discordantly 1 time ---- 1192486 pairs aligned 0 times concordantly or discordantly; of these: 2384972 mates make up the pairs; of these: 1825538 (76.54%) aligned 0 times 113517 (4.76%) aligned exactly 1 time 445917 (18.70%) aligned >1 times 90.11% overall alignment rate Time searching: 00:05:39 Overall time: 00:05:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4997103 / 8016482 = 0.6234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:46:11: # Command line: callpeak -t SRX3544074.bam -f BAM -g 12100000 -n SRX3544074.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3544074.20 # format = BAM # ChIP-seq file = ['SRX3544074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:46:11: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:46:11: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:46:11: # Command line: callpeak -t SRX3544074.bam -f BAM -g 12100000 -n SRX3544074.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3544074.05 # format = BAM # ChIP-seq file = ['SRX3544074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:46:11: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:46:11: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:46:11: # Command line: callpeak -t SRX3544074.bam -f BAM -g 12100000 -n SRX3544074.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3544074.10 # format = BAM # ChIP-seq file = ['SRX3544074.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:46:11: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:46:11: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:46:17: 1000000 INFO @ Sat, 15 Sep 2018 10:46:17: 1000000 INFO @ Sat, 15 Sep 2018 10:46:17: 1000000 INFO @ Sat, 15 Sep 2018 10:46:22: 2000000 INFO @ Sat, 15 Sep 2018 10:46:23: 2000000 INFO @ Sat, 15 Sep 2018 10:46:23: 2000000 INFO @ Sat, 15 Sep 2018 10:46:28: 3000000 INFO @ Sat, 15 Sep 2018 10:46:29: 3000000 INFO @ Sat, 15 Sep 2018 10:46:29: 3000000 INFO @ Sat, 15 Sep 2018 10:46:33: 4000000 INFO @ Sat, 15 Sep 2018 10:46:35: 4000000 INFO @ Sat, 15 Sep 2018 10:46:35: 4000000 INFO @ Sat, 15 Sep 2018 10:46:39: 5000000 INFO @ Sat, 15 Sep 2018 10:46:41: 5000000 INFO @ Sat, 15 Sep 2018 10:46:41: 5000000 INFO @ Sat, 15 Sep 2018 10:46:44: 6000000 INFO @ Sat, 15 Sep 2018 10:46:47: 6000000 INFO @ Sat, 15 Sep 2018 10:46:48: 6000000 INFO @ Sat, 15 Sep 2018 10:46:48: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:46:48: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:46:48: #1 total tags in treatment: 2992184 INFO @ Sat, 15 Sep 2018 10:46:48: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:46:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:46:48: #1 tags after filtering in treatment: 1842518 INFO @ Sat, 15 Sep 2018 10:46:48: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Sep 2018 10:46:48: #1 finished! INFO @ Sat, 15 Sep 2018 10:46:48: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:46:48: #2 number of paired peaks: 39 WARNING @ Sat, 15 Sep 2018 10:46:48: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:46:48: Process for pairing-model is terminated! cat: SRX3544074.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544074.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:46:51: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:46:51: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:46:51: #1 total tags in treatment: 2992184 INFO @ Sat, 15 Sep 2018 10:46:51: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:46:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:46:51: #1 tags after filtering in treatment: 1842518 INFO @ Sat, 15 Sep 2018 10:46:51: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Sep 2018 10:46:51: #1 finished! INFO @ Sat, 15 Sep 2018 10:46:51: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:46:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:46:51: #2 number of paired peaks: 39 WARNING @ Sat, 15 Sep 2018 10:46:51: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:46:51: Process for pairing-model is terminated! cat: SRX3544074.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544074.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:46:52: #1 tag size is determined as 35 bps INFO @ Sat, 15 Sep 2018 10:46:52: #1 tag size = 35 INFO @ Sat, 15 Sep 2018 10:46:52: #1 total tags in treatment: 2992184 INFO @ Sat, 15 Sep 2018 10:46:52: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:46:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:46:52: #1 tags after filtering in treatment: 1842518 INFO @ Sat, 15 Sep 2018 10:46:52: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Sep 2018 10:46:52: #1 finished! INFO @ Sat, 15 Sep 2018 10:46:52: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:46:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:46:52: #2 number of paired peaks: 39 WARNING @ Sat, 15 Sep 2018 10:46:52: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:46:52: Process for pairing-model is terminated! cat: SRX3544074.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3544074.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3544074.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。