Job ID = 14521982 SRX = SRX3471501 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 52975451 spots for SRR6376708/SRR6376708.sra Written 52975451 spots for SRR6376708/SRR6376708.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:01 52975451 reads; of these: 52975451 (100.00%) were unpaired; of these: 204260 (0.39%) aligned 0 times 52006584 (98.17%) aligned exactly 1 time 764607 (1.44%) aligned >1 times 99.61% overall alignment rate Time searching: 00:04:01 Overall time: 00:04:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 38109043 / 52771191 = 0.7222 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:13:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:13:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:13:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:13:07: 1000000 INFO @ Sat, 15 Jan 2022 22:13:12: 2000000 INFO @ Sat, 15 Jan 2022 22:13:18: 3000000 INFO @ Sat, 15 Jan 2022 22:13:23: 4000000 INFO @ Sat, 15 Jan 2022 22:13:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:13:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:13:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:13:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:13:34: 6000000 INFO @ Sat, 15 Jan 2022 22:13:38: 1000000 INFO @ Sat, 15 Jan 2022 22:13:40: 7000000 INFO @ Sat, 15 Jan 2022 22:13:44: 2000000 INFO @ Sat, 15 Jan 2022 22:13:46: 8000000 INFO @ Sat, 15 Jan 2022 22:13:50: 3000000 INFO @ Sat, 15 Jan 2022 22:13:52: 9000000 INFO @ Sat, 15 Jan 2022 22:13:56: 4000000 INFO @ Sat, 15 Jan 2022 22:13:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:14:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:14:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:14:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:14:03: 5000000 INFO @ Sat, 15 Jan 2022 22:14:04: 11000000 INFO @ Sat, 15 Jan 2022 22:14:09: 1000000 INFO @ Sat, 15 Jan 2022 22:14:09: 6000000 INFO @ Sat, 15 Jan 2022 22:14:11: 12000000 INFO @ Sat, 15 Jan 2022 22:14:16: 7000000 INFO @ Sat, 15 Jan 2022 22:14:17: 2000000 INFO @ Sat, 15 Jan 2022 22:14:18: 13000000 INFO @ Sat, 15 Jan 2022 22:14:23: 8000000 INFO @ Sat, 15 Jan 2022 22:14:24: 3000000 INFO @ Sat, 15 Jan 2022 22:14:25: 14000000 INFO @ Sat, 15 Jan 2022 22:14:29: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:14:29: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:14:29: #1 total tags in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:14:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:14:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:14:29: #1 tags after filtering in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:14:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:14:29: #1 finished! INFO @ Sat, 15 Jan 2022 22:14:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:14:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:14:30: 9000000 INFO @ Sat, 15 Jan 2022 22:14:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:14:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:14:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:14:32: 4000000 INFO @ Sat, 15 Jan 2022 22:14:37: 10000000 INFO @ Sat, 15 Jan 2022 22:14:39: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:14:44: 11000000 INFO @ Sat, 15 Jan 2022 22:14:47: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:14:50: 12000000 INFO @ Sat, 15 Jan 2022 22:14:54: 7000000 INFO @ Sat, 15 Jan 2022 22:14:57: 13000000 INFO @ Sat, 15 Jan 2022 22:15:01: 8000000 INFO @ Sat, 15 Jan 2022 22:15:04: 14000000 INFO @ Sat, 15 Jan 2022 22:15:08: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:15:08: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:15:08: #1 total tags in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:15:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:09: #1 tags after filtering in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:15:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:15:09: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:09: 9000000 INFO @ Sat, 15 Jan 2022 22:15:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:15:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:15:16: 10000000 INFO @ Sat, 15 Jan 2022 22:15:23: 11000000 INFO @ Sat, 15 Jan 2022 22:15:29: 12000000 INFO @ Sat, 15 Jan 2022 22:15:36: 13000000 INFO @ Sat, 15 Jan 2022 22:15:43: 14000000 INFO @ Sat, 15 Jan 2022 22:15:47: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:15:47: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:15:47: #1 total tags in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:15:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:47: #1 tags after filtering in treatment: 14662148 INFO @ Sat, 15 Jan 2022 22:15:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:15:47: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:15:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471501/SRX3471501.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling