Job ID = 14521981 SRX = SRX3471500 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8128086 spots for SRR6376707/SRR6376707.sra Written 8128086 spots for SRR6376707/SRR6376707.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 8128086 reads; of these: 8128086 (100.00%) were unpaired; of these: 30758 (0.38%) aligned 0 times 7978189 (98.16%) aligned exactly 1 time 119139 (1.47%) aligned >1 times 99.62% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3308182 / 8097328 = 0.4086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:32: 1000000 INFO @ Sat, 15 Jan 2022 22:02:38: 2000000 INFO @ Sat, 15 Jan 2022 22:02:45: 3000000 INFO @ Sat, 15 Jan 2022 22:02:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:58: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:02:58: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:02:58: #1 total tags in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:02:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:02:58: #1 tags after filtering in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:02:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:02:58: #1 finished! INFO @ Sat, 15 Jan 2022 22:02:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:02:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:02:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:02:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:02:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:02: 1000000 INFO @ Sat, 15 Jan 2022 22:03:09: 2000000 INFO @ Sat, 15 Jan 2022 22:03:16: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:23: 4000000 INFO @ Sat, 15 Jan 2022 22:03:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:28: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:03:28: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:03:28: #1 total tags in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:03:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:28: #1 tags after filtering in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:03:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:03:28: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:32: 1000000 INFO @ Sat, 15 Jan 2022 22:03:39: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:03:47: 3000000 INFO @ Sat, 15 Jan 2022 22:03:54: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:03:59: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 22:03:59: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 22:03:59: #1 total tags in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:03:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:59: #1 tags after filtering in treatment: 4789146 INFO @ Sat, 15 Jan 2022 22:03:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 22:03:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3471500/SRX3471500.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling