Job ID = 4289035 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,549,020 reads read : 9,549,020 reads written : 9,549,020 spots read : 9,353,634 reads read : 9,353,634 reads written : 9,353,634 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:31 18902654 reads; of these: 18902654 (100.00%) were unpaired; of these: 484253 (2.56%) aligned 0 times 16488894 (87.23%) aligned exactly 1 time 1929507 (10.21%) aligned >1 times 97.44% overall alignment rate Time searching: 00:03:31 Overall time: 00:03:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7343269 / 18418401 = 0.3987 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Tue, 10 Dec 2019 13:37:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:37:42: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:37:42: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:37:49: 1000000 INFO @ Tue, 10 Dec 2019 13:37:56: 2000000 INFO @ Tue, 10 Dec 2019 13:38:03: 3000000 INFO @ Tue, 10 Dec 2019 13:38:10: 4000000 INFO @ Tue, 10 Dec 2019 13:38:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:38:11: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:38:11: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:38:17: 5000000 INFO @ Tue, 10 Dec 2019 13:38:20: 1000000 INFO @ Tue, 10 Dec 2019 13:38:25: 6000000 INFO @ Tue, 10 Dec 2019 13:38:28: 2000000 INFO @ Tue, 10 Dec 2019 13:38:32: 7000000 INFO @ Tue, 10 Dec 2019 13:38:36: 3000000 BedGraph に変換中... INFO @ Tue, 10 Dec 2019 13:38:39: 8000000 INFO @ Tue, 10 Dec 2019 13:38:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 10 Dec 2019 13:38:41: #1 read tag files... INFO @ Tue, 10 Dec 2019 13:38:41: #1 read treatment tags... INFO @ Tue, 10 Dec 2019 13:38:44: 4000000 INFO @ Tue, 10 Dec 2019 13:38:46: 9000000 INFO @ Tue, 10 Dec 2019 13:38:50: 1000000 INFO @ Tue, 10 Dec 2019 13:38:52: 5000000 INFO @ Tue, 10 Dec 2019 13:38:53: 10000000 INFO @ Tue, 10 Dec 2019 13:38:59: 2000000 INFO @ Tue, 10 Dec 2019 13:39:01: 11000000 INFO @ Tue, 10 Dec 2019 13:39:01: 6000000 INFO @ Tue, 10 Dec 2019 13:39:01: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 13:39:01: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 13:39:01: #1 total tags in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:39:01: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:39:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:39:01: #1 tags after filtering in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:39:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:39:01: #1 finished! INFO @ Tue, 10 Dec 2019 13:39:01: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:39:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:39:02: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:39:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:39:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:39:07: 3000000 INFO @ Tue, 10 Dec 2019 13:39:09: 7000000 INFO @ Tue, 10 Dec 2019 13:39:16: 4000000 INFO @ Tue, 10 Dec 2019 13:39:17: 8000000 INFO @ Tue, 10 Dec 2019 13:39:24: 5000000 INFO @ Tue, 10 Dec 2019 13:39:25: 9000000 INFO @ Tue, 10 Dec 2019 13:39:32: 6000000 INFO @ Tue, 10 Dec 2019 13:39:33: 10000000 INFO @ Tue, 10 Dec 2019 13:39:40: 7000000 INFO @ Tue, 10 Dec 2019 13:39:41: 11000000 INFO @ Tue, 10 Dec 2019 13:39:42: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 13:39:42: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 13:39:42: #1 total tags in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:39:42: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:39:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:39:42: #1 tags after filtering in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:39:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:39:42: #1 finished! INFO @ Tue, 10 Dec 2019 13:39:42: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:39:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:39:43: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:39:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:39:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 10 Dec 2019 13:39:49: 8000000 INFO @ Tue, 10 Dec 2019 13:39:57: 9000000 INFO @ Tue, 10 Dec 2019 13:40:05: 10000000 INFO @ Tue, 10 Dec 2019 13:40:13: 11000000 INFO @ Tue, 10 Dec 2019 13:40:14: #1 tag size is determined as 51 bps INFO @ Tue, 10 Dec 2019 13:40:14: #1 tag size = 51 INFO @ Tue, 10 Dec 2019 13:40:14: #1 total tags in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:40:14: #1 user defined the maximum tags... INFO @ Tue, 10 Dec 2019 13:40:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 10 Dec 2019 13:40:14: #1 tags after filtering in treatment: 11075132 INFO @ Tue, 10 Dec 2019 13:40:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 10 Dec 2019 13:40:14: #1 finished! INFO @ Tue, 10 Dec 2019 13:40:14: #2 Build Peak Model... INFO @ Tue, 10 Dec 2019 13:40:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 10 Dec 2019 13:40:15: #2 number of paired peaks: 0 WARNING @ Tue, 10 Dec 2019 13:40:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 10 Dec 2019 13:40:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3463338/SRX3463338.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。