Job ID = 10937670 sra ファイルのダウンロード中... Completed: 310604K bytes transferred in 12 seconds (200383K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 9461907 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3463314/SRR6367853.sra Written 9461907 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3463314/SRR6367853.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:49 9461907 reads; of these: 9461907 (100.00%) were unpaired; of these: 640635 (6.77%) aligned 0 times 7838407 (82.84%) aligned exactly 1 time 982865 (10.39%) aligned >1 times 93.23% overall alignment rate Time searching: 00:01:49 Overall time: 00:01:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2263738 / 8821272 = 0.2566 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 10 Aug 2018 02:50:54: # Command line: callpeak -t SRX3463314.bam -f BAM -g 12100000 -n SRX3463314.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3463314.10 # format = BAM # ChIP-seq file = ['SRX3463314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:50:54: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:50:54: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:50:54: # Command line: callpeak -t SRX3463314.bam -f BAM -g 12100000 -n SRX3463314.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3463314.20 # format = BAM # ChIP-seq file = ['SRX3463314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:50:54: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:50:54: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:50:54: # Command line: callpeak -t SRX3463314.bam -f BAM -g 12100000 -n SRX3463314.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3463314.05 # format = BAM # ChIP-seq file = ['SRX3463314.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:50:54: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:50:54: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:51:02: 1000000 INFO @ Fri, 10 Aug 2018 02:51:04: 1000000 INFO @ Fri, 10 Aug 2018 02:51:04: 1000000 INFO @ Fri, 10 Aug 2018 02:51:11: 2000000 INFO @ Fri, 10 Aug 2018 02:51:14: 2000000 INFO @ Fri, 10 Aug 2018 02:51:14: 2000000 INFO @ Fri, 10 Aug 2018 02:51:19: 3000000 INFO @ Fri, 10 Aug 2018 02:51:23: 3000000 INFO @ Fri, 10 Aug 2018 02:51:23: 3000000 INFO @ Fri, 10 Aug 2018 02:51:27: 4000000 INFO @ Fri, 10 Aug 2018 02:51:33: 4000000 INFO @ Fri, 10 Aug 2018 02:51:33: 4000000 INFO @ Fri, 10 Aug 2018 02:51:35: 5000000 INFO @ Fri, 10 Aug 2018 02:51:43: 5000000 INFO @ Fri, 10 Aug 2018 02:51:43: 5000000 INFO @ Fri, 10 Aug 2018 02:51:43: 6000000 INFO @ Fri, 10 Aug 2018 02:51:47: #1 tag size is determined as 51 bps INFO @ Fri, 10 Aug 2018 02:51:47: #1 tag size = 51 INFO @ Fri, 10 Aug 2018 02:51:47: #1 total tags in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:47: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:51:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:51:48: #1 tags after filtering in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:51:48: #1 finished! INFO @ Fri, 10 Aug 2018 02:51:48: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:51:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:51:48: #2 number of paired peaks: 0 WARNING @ Fri, 10 Aug 2018 02:51:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Aug 2018 02:51:48: Process for pairing-model is terminated! cat: SRX3463314.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3463314.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 10 Aug 2018 02:51:52: 6000000 INFO @ Fri, 10 Aug 2018 02:51:53: 6000000 INFO @ Fri, 10 Aug 2018 02:51:57: #1 tag size is determined as 51 bps INFO @ Fri, 10 Aug 2018 02:51:57: #1 tag size = 51 INFO @ Fri, 10 Aug 2018 02:51:57: #1 total tags in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:57: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:51:57: #1 tags after filtering in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:51:57: #1 finished! INFO @ Fri, 10 Aug 2018 02:51:57: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:51:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:51:57: #2 number of paired peaks: 0 WARNING @ Fri, 10 Aug 2018 02:51:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Aug 2018 02:51:57: Process for pairing-model is terminated! cat: SRX3463314.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3463314.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 10 Aug 2018 02:51:58: #1 tag size is determined as 51 bps INFO @ Fri, 10 Aug 2018 02:51:58: #1 tag size = 51 INFO @ Fri, 10 Aug 2018 02:51:58: #1 total tags in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:58: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:51:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:51:58: #1 tags after filtering in treatment: 6557534 INFO @ Fri, 10 Aug 2018 02:51:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:51:58: #1 finished! INFO @ Fri, 10 Aug 2018 02:51:58: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:51:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:51:58: #2 number of paired peaks: 0 WARNING @ Fri, 10 Aug 2018 02:51:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Aug 2018 02:51:58: Process for pairing-model is terminated! cat: SRX3463314.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3463314.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3463314.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。