Job ID = 10937643 sra ファイルのダウンロード中... Completed: 170663K bytes transferred in 6 seconds (221137K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 5309331 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3463294/SRR6367833.sra Written 5309331 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3463294/SRR6367833.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:04 5309331 reads; of these: 5309331 (100.00%) were unpaired; of these: 709313 (13.36%) aligned 0 times 4070348 (76.66%) aligned exactly 1 time 529670 (9.98%) aligned >1 times 86.64% overall alignment rate Time searching: 00:01:04 Overall time: 00:01:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3243367 / 4600018 = 0.7051 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 10 Aug 2018 02:45:31: # Command line: callpeak -t SRX3463294.bam -f BAM -g 12100000 -n SRX3463294.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3463294.05 # format = BAM # ChIP-seq file = ['SRX3463294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:45:31: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:45:31: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:45:31: # Command line: callpeak -t SRX3463294.bam -f BAM -g 12100000 -n SRX3463294.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3463294.20 # format = BAM # ChIP-seq file = ['SRX3463294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:45:31: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:45:31: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:45:31: # Command line: callpeak -t SRX3463294.bam -f BAM -g 12100000 -n SRX3463294.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3463294.10 # format = BAM # ChIP-seq file = ['SRX3463294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Aug 2018 02:45:31: #1 read tag files... INFO @ Fri, 10 Aug 2018 02:45:31: #1 read treatment tags... INFO @ Fri, 10 Aug 2018 02:45:40: 1000000 INFO @ Fri, 10 Aug 2018 02:45:41: 1000000 INFO @ Fri, 10 Aug 2018 02:45:41: 1000000 INFO @ Fri, 10 Aug 2018 02:45:43: #1 tag size is determined as 50 bps INFO @ Fri, 10 Aug 2018 02:45:43: #1 tag size = 50 INFO @ Fri, 10 Aug 2018 02:45:43: #1 total tags in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:43: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:45:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:45:43: #1 tags after filtering in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:45:43: #1 finished! INFO @ Fri, 10 Aug 2018 02:45:43: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:45:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:45:43: #2 number of paired peaks: 235 WARNING @ Fri, 10 Aug 2018 02:45:43: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Aug 2018 02:45:43: start model_add_line... INFO @ Fri, 10 Aug 2018 02:45:43: start X-correlation... INFO @ Fri, 10 Aug 2018 02:45:43: end of X-cor INFO @ Fri, 10 Aug 2018 02:45:43: #2 finished! INFO @ Fri, 10 Aug 2018 02:45:43: #2 predicted fragment length is 121 bps INFO @ Fri, 10 Aug 2018 02:45:43: #2 alternative fragment length(s) may be 121 bps INFO @ Fri, 10 Aug 2018 02:45:43: #2.2 Generate R script for model : SRX3463294.20_model.r INFO @ Fri, 10 Aug 2018 02:45:43: #3 Call peaks... INFO @ Fri, 10 Aug 2018 02:45:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Aug 2018 02:45:44: #1 tag size is determined as 50 bps INFO @ Fri, 10 Aug 2018 02:45:44: #1 tag size = 50 INFO @ Fri, 10 Aug 2018 02:45:44: #1 total tags in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:44: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:45:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:45:44: #1 tags after filtering in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:45:44: #1 finished! INFO @ Fri, 10 Aug 2018 02:45:44: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:45:44: #2 number of paired peaks: 235 WARNING @ Fri, 10 Aug 2018 02:45:44: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Aug 2018 02:45:44: start model_add_line... INFO @ Fri, 10 Aug 2018 02:45:44: start X-correlation... INFO @ Fri, 10 Aug 2018 02:45:44: end of X-cor INFO @ Fri, 10 Aug 2018 02:45:44: #2 finished! INFO @ Fri, 10 Aug 2018 02:45:44: #2 predicted fragment length is 121 bps INFO @ Fri, 10 Aug 2018 02:45:44: #2 alternative fragment length(s) may be 121 bps INFO @ Fri, 10 Aug 2018 02:45:44: #2.2 Generate R script for model : SRX3463294.05_model.r INFO @ Fri, 10 Aug 2018 02:45:44: #3 Call peaks... INFO @ Fri, 10 Aug 2018 02:45:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Aug 2018 02:45:44: #1 tag size is determined as 50 bps INFO @ Fri, 10 Aug 2018 02:45:44: #1 tag size = 50 INFO @ Fri, 10 Aug 2018 02:45:44: #1 total tags in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:44: #1 user defined the maximum tags... INFO @ Fri, 10 Aug 2018 02:45:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Aug 2018 02:45:44: #1 tags after filtering in treatment: 1356651 INFO @ Fri, 10 Aug 2018 02:45:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 10 Aug 2018 02:45:44: #1 finished! INFO @ Fri, 10 Aug 2018 02:45:44: #2 Build Peak Model... INFO @ Fri, 10 Aug 2018 02:45:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Aug 2018 02:45:44: #2 number of paired peaks: 235 WARNING @ Fri, 10 Aug 2018 02:45:44: Fewer paired peaks (235) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 235 pairs to build model! INFO @ Fri, 10 Aug 2018 02:45:44: start model_add_line... INFO @ Fri, 10 Aug 2018 02:45:44: start X-correlation... INFO @ Fri, 10 Aug 2018 02:45:44: end of X-cor INFO @ Fri, 10 Aug 2018 02:45:44: #2 finished! INFO @ Fri, 10 Aug 2018 02:45:44: #2 predicted fragment length is 121 bps INFO @ Fri, 10 Aug 2018 02:45:44: #2 alternative fragment length(s) may be 121 bps INFO @ Fri, 10 Aug 2018 02:45:44: #2.2 Generate R script for model : SRX3463294.10_model.r INFO @ Fri, 10 Aug 2018 02:45:44: #3 Call peaks... INFO @ Fri, 10 Aug 2018 02:45:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Aug 2018 02:45:48: #3 Call peaks for each chromosome... INFO @ Fri, 10 Aug 2018 02:45:48: #3 Call peaks for each chromosome... INFO @ Fri, 10 Aug 2018 02:45:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write output xls file... SRX3463294.20_peaks.xls INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write peak in narrowPeak format file... SRX3463294.20_peaks.narrowPeak INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write summits bed file... SRX3463294.20_summits.bed INFO @ Fri, 10 Aug 2018 02:45:50: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (386 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write output xls file... SRX3463294.05_peaks.xls INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write peak in narrowPeak format file... SRX3463294.05_peaks.narrowPeak INFO @ Fri, 10 Aug 2018 02:45:50: #4 Write summits bed file... SRX3463294.05_summits.bed INFO @ Fri, 10 Aug 2018 02:45:50: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1261 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 10 Aug 2018 02:45:51: #4 Write output xls file... SRX3463294.10_peaks.xls INFO @ Fri, 10 Aug 2018 02:45:51: #4 Write peak in narrowPeak format file... SRX3463294.10_peaks.narrowPeak INFO @ Fri, 10 Aug 2018 02:45:51: #4 Write summits bed file... SRX3463294.10_summits.bed INFO @ Fri, 10 Aug 2018 02:45:51: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (725 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。