Job ID = 10488220 sra ファイルのダウンロード中... Completed: 84163K bytes transferred in 11 seconds (59170K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 3497823 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3451537/SRR6354927.sra Written 3497823 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:32 3497823 reads; of these: 3497823 (100.00%) were unpaired; of these: 287802 (8.23%) aligned 0 times 1995236 (57.04%) aligned exactly 1 time 1214785 (34.73%) aligned >1 times 91.77% overall alignment rate Time searching: 00:00:32 Overall time: 00:00:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2273526 / 3210021 = 0.7083 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 18 Mar 2018 12:20:27: # Command line: callpeak -t SRX3451537.bam -f BAM -g 12100000 -n SRX3451537.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3451537.20 # format = BAM # ChIP-seq file = ['SRX3451537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 18 Mar 2018 12:20:27: #1 read tag files... INFO @ Sun, 18 Mar 2018 12:20:27: #1 read treatment tags... INFO @ Sun, 18 Mar 2018 12:20:27: # Command line: callpeak -t SRX3451537.bam -f BAM -g 12100000 -n SRX3451537.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3451537.05 # format = BAM # ChIP-seq file = ['SRX3451537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 18 Mar 2018 12:20:27: #1 read tag files... INFO @ Sun, 18 Mar 2018 12:20:27: #1 read treatment tags... INFO @ Sun, 18 Mar 2018 12:20:27: # Command line: callpeak -t SRX3451537.bam -f BAM -g 12100000 -n SRX3451537.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3451537.10 # format = BAM # ChIP-seq file = ['SRX3451537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 18 Mar 2018 12:20:27: #1 read tag files... INFO @ Sun, 18 Mar 2018 12:20:27: #1 read treatment tags... INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size is determined as 40 bps INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size = 40 INFO @ Sun, 18 Mar 2018 12:20:34: #1 total tags in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 user defined the maximum tags... INFO @ Sun, 18 Mar 2018 12:20:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 18 Mar 2018 12:20:34: #1 tags after filtering in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 18 Mar 2018 12:20:34: #1 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 Build Peak Model... INFO @ Sun, 18 Mar 2018 12:20:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #2 number of paired peaks: 398 WARNING @ Sun, 18 Mar 2018 12:20:34: Fewer paired peaks (398) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 398 pairs to build model! INFO @ Sun, 18 Mar 2018 12:20:34: start model_add_line... INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size is determined as 40 bps INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size = 40 INFO @ Sun, 18 Mar 2018 12:20:34: #1 total tags in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 user defined the maximum tags... INFO @ Sun, 18 Mar 2018 12:20:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size is determined as 40 bps INFO @ Sun, 18 Mar 2018 12:20:34: #1 tag size = 40 INFO @ Sun, 18 Mar 2018 12:20:34: #1 total tags in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 user defined the maximum tags... INFO @ Sun, 18 Mar 2018 12:20:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 18 Mar 2018 12:20:34: start X-correlation... INFO @ Sun, 18 Mar 2018 12:20:34: end of X-cor INFO @ Sun, 18 Mar 2018 12:20:34: #2 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 predicted fragment length is 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2 alternative fragment length(s) may be 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2.2 Generate R script for model : SRX3451537.10_model.r INFO @ Sun, 18 Mar 2018 12:20:34: #3 Call peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 18 Mar 2018 12:20:34: #1 tags after filtering in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 18 Mar 2018 12:20:34: #1 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 Build Peak Model... INFO @ Sun, 18 Mar 2018 12:20:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #1 tags after filtering in treatment: 936495 INFO @ Sun, 18 Mar 2018 12:20:34: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 18 Mar 2018 12:20:34: #1 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 Build Peak Model... INFO @ Sun, 18 Mar 2018 12:20:34: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #2 number of paired peaks: 398 WARNING @ Sun, 18 Mar 2018 12:20:34: Fewer paired peaks (398) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 398 pairs to build model! INFO @ Sun, 18 Mar 2018 12:20:34: start model_add_line... INFO @ Sun, 18 Mar 2018 12:20:34: #2 number of paired peaks: 398 WARNING @ Sun, 18 Mar 2018 12:20:34: Fewer paired peaks (398) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 398 pairs to build model! INFO @ Sun, 18 Mar 2018 12:20:34: start model_add_line... INFO @ Sun, 18 Mar 2018 12:20:34: start X-correlation... INFO @ Sun, 18 Mar 2018 12:20:34: start X-correlation... INFO @ Sun, 18 Mar 2018 12:20:34: end of X-cor INFO @ Sun, 18 Mar 2018 12:20:34: end of X-cor INFO @ Sun, 18 Mar 2018 12:20:34: #2 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 finished! INFO @ Sun, 18 Mar 2018 12:20:34: #2 predicted fragment length is 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2 predicted fragment length is 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2 alternative fragment length(s) may be 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2 alternative fragment length(s) may be 132 bps INFO @ Sun, 18 Mar 2018 12:20:34: #2.2 Generate R script for model : SRX3451537.05_model.r INFO @ Sun, 18 Mar 2018 12:20:34: #2.2 Generate R script for model : SRX3451537.20_model.r INFO @ Sun, 18 Mar 2018 12:20:34: #3 Call peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 18 Mar 2018 12:20:34: #3 Call peaks... INFO @ Sun, 18 Mar 2018 12:20:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 18 Mar 2018 12:20:37: #3 Call peaks for each chromosome... INFO @ Sun, 18 Mar 2018 12:20:37: #3 Call peaks for each chromosome... INFO @ Sun, 18 Mar 2018 12:20:37: #3 Call peaks for each chromosome... INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write output xls file... SRX3451537.10_peaks.xls INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write peak in narrowPeak format file... SRX3451537.10_peaks.narrowPeak INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write summits bed file... SRX3451537.10_summits.bed INFO @ Sun, 18 Mar 2018 12:20:38: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (820 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write output xls file... SRX3451537.05_peaks.xls INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write peak in narrowPeak format file... SRX3451537.05_peaks.narrowPeak INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write summits bed file... SRX3451537.05_summits.bed INFO @ Sun, 18 Mar 2018 12:20:38: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (1173 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write output xls file... SRX3451537.20_peaks.xls INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write peak in narrowPeak format file... SRX3451537.20_peaks.narrowPeak INFO @ Sun, 18 Mar 2018 12:20:38: #4 Write summits bed file... SRX3451537.20_summits.bed INFO @ Sun, 18 Mar 2018 12:20:38: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (613 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。