Job ID = 2640840 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,079,456 reads read : 10,079,456 reads written : 10,079,456 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322066.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 10079456 reads; of these: 10079456 (100.00%) were unpaired; of these: 1148790 (11.40%) aligned 0 times 7856042 (77.94%) aligned exactly 1 time 1074624 (10.66%) aligned >1 times 88.60% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1892515 / 8930666 = 0.2119 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:31:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:27: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:27: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:36: 1000000 INFO @ Sat, 24 Aug 2019 19:31:45: 2000000 INFO @ Sat, 24 Aug 2019 19:31:54: 3000000 INFO @ Sat, 24 Aug 2019 19:31:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:57: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:57: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:32:03: 4000000 INFO @ Sat, 24 Aug 2019 19:32:06: 1000000 INFO @ Sat, 24 Aug 2019 19:32:12: 5000000 INFO @ Sat, 24 Aug 2019 19:32:15: 2000000 INFO @ Sat, 24 Aug 2019 19:32:21: 6000000 INFO @ Sat, 24 Aug 2019 19:32:24: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:32:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:32:27: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:32:27: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:32:30: 7000000 INFO @ Sat, 24 Aug 2019 19:32:30: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:32:30: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:32:30: #1 total tags in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:32:30: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:32:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:32:30: #1 tags after filtering in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:32:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:32:30: #1 finished! INFO @ Sat, 24 Aug 2019 19:32:30: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:32:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:32:31: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:32:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:32:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:32:32: 4000000 INFO @ Sat, 24 Aug 2019 19:32:36: 1000000 INFO @ Sat, 24 Aug 2019 19:32:40: 5000000 INFO @ Sat, 24 Aug 2019 19:32:46: 2000000 INFO @ Sat, 24 Aug 2019 19:32:49: 6000000 INFO @ Sat, 24 Aug 2019 19:32:56: 3000000 INFO @ Sat, 24 Aug 2019 19:33:00: 7000000 INFO @ Sat, 24 Aug 2019 19:33:01: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:33:01: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:33:01: #1 total tags in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:33:01: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:33:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:33:01: #1 tags after filtering in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:33:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:33:01: #1 finished! INFO @ Sat, 24 Aug 2019 19:33:01: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:33:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:33:01: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:33:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:33:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:33:06: 4000000 INFO @ Sat, 24 Aug 2019 19:33:13: 5000000 INFO @ Sat, 24 Aug 2019 19:33:20: 6000000 INFO @ Sat, 24 Aug 2019 19:33:27: 7000000 INFO @ Sat, 24 Aug 2019 19:33:27: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:33:27: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:33:27: #1 total tags in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:33:27: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:33:27: #1 tags after filtering in treatment: 7038151 INFO @ Sat, 24 Aug 2019 19:33:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:33:27: #1 finished! INFO @ Sat, 24 Aug 2019 19:33:27: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:33:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:33:28: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:33:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:33:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421889/SRX3421889.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。