Job ID = 2640837 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,189,240 reads read : 8,189,240 reads written : 8,189,240 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322064.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:54 8189240 reads; of these: 8189240 (100.00%) were unpaired; of these: 257798 (3.15%) aligned 0 times 6955719 (84.94%) aligned exactly 1 time 975723 (11.91%) aligned >1 times 96.85% overall alignment rate Time searching: 00:01:54 Overall time: 00:01:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1581889 / 7931442 = 0.1994 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:30:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:59: 1000000 INFO @ Sat, 24 Aug 2019 19:31:06: 2000000 INFO @ Sat, 24 Aug 2019 19:31:13: 3000000 INFO @ Sat, 24 Aug 2019 19:31:20: 4000000 INFO @ Sat, 24 Aug 2019 19:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:21: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:21: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:28: 5000000 INFO @ Sat, 24 Aug 2019 19:31:29: 1000000 INFO @ Sat, 24 Aug 2019 19:31:35: 6000000 INFO @ Sat, 24 Aug 2019 19:31:36: 2000000 INFO @ Sat, 24 Aug 2019 19:31:38: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:31:38: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:31:38: #1 total tags in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:31:38: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:38: #1 tags after filtering in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:31:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:38: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:38: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:38: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:31:42: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:31:49: 4000000 INFO @ Sat, 24 Aug 2019 19:31:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:56: 5000000 INFO @ Sat, 24 Aug 2019 19:31:59: 1000000 INFO @ Sat, 24 Aug 2019 19:32:03: 6000000 INFO @ Sat, 24 Aug 2019 19:32:06: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:32:06: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:32:06: #1 total tags in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:32:06: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:32:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:32:06: #1 tags after filtering in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:32:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:32:06: #1 finished! INFO @ Sat, 24 Aug 2019 19:32:06: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:32:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:32:07: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:32:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:32:07: Process for pairing-model is terminated! INFO @ Sat, 24 Aug 2019 19:32:07: 2000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:32:14: 3000000 INFO @ Sat, 24 Aug 2019 19:32:21: 4000000 INFO @ Sat, 24 Aug 2019 19:32:28: 5000000 INFO @ Sat, 24 Aug 2019 19:32:36: 6000000 INFO @ Sat, 24 Aug 2019 19:32:38: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:32:38: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:32:38: #1 total tags in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:32:38: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:32:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:32:38: #1 tags after filtering in treatment: 6349553 INFO @ Sat, 24 Aug 2019 19:32:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:32:38: #1 finished! INFO @ Sat, 24 Aug 2019 19:32:38: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:32:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:32:39: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:32:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:32:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421887/SRX3421887.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。