Job ID = 2640836 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,667,422 reads read : 8,667,422 reads written : 8,667,422 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322063.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:57 8667422 reads; of these: 8667422 (100.00%) were unpaired; of these: 240186 (2.77%) aligned 0 times 7354571 (84.85%) aligned exactly 1 time 1072665 (12.38%) aligned >1 times 97.23% overall alignment rate Time searching: 00:01:57 Overall time: 00:01:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1757911 / 8427236 = 0.2086 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:30:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:36: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:36: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:43: 1000000 INFO @ Sat, 24 Aug 2019 19:30:50: 2000000 INFO @ Sat, 24 Aug 2019 19:30:56: 3000000 INFO @ Sat, 24 Aug 2019 19:31:03: 4000000 INFO @ Sat, 24 Aug 2019 19:31:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:06: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:06: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:10: 5000000 INFO @ Sat, 24 Aug 2019 19:31:14: 1000000 INFO @ Sat, 24 Aug 2019 19:31:16: 6000000 INFO @ Sat, 24 Aug 2019 19:31:21: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:31:21: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:31:21: #1 total tags in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:31:21: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:21: #1 tags after filtering in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:21: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:21: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:22: 2000000 INFO @ Sat, 24 Aug 2019 19:31:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:31:29: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:31:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:36: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:36: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:36: 4000000 INFO @ Sat, 24 Aug 2019 19:31:43: 1000000 INFO @ Sat, 24 Aug 2019 19:31:44: 5000000 INFO @ Sat, 24 Aug 2019 19:31:50: 2000000 INFO @ Sat, 24 Aug 2019 19:31:51: 6000000 INFO @ Sat, 24 Aug 2019 19:31:56: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:31:56: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:31:56: #1 total tags in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:31:56: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:56: #1 tags after filtering in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:31:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:56: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:56: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:57: 3000000 INFO @ Sat, 24 Aug 2019 19:31:57: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:32:03: 4000000 INFO @ Sat, 24 Aug 2019 19:32:10: 5000000 INFO @ Sat, 24 Aug 2019 19:32:17: 6000000 INFO @ Sat, 24 Aug 2019 19:32:21: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:32:21: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:32:21: #1 total tags in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:32:21: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:32:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:32:21: #1 tags after filtering in treatment: 6669325 INFO @ Sat, 24 Aug 2019 19:32:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:32:21: #1 finished! INFO @ Sat, 24 Aug 2019 19:32:21: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:32:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:32:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:32:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:32:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421886/SRX3421886.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。