Job ID = 2640835 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,942,268 reads read : 8,942,268 reads written : 8,942,268 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322062.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:44 8942268 reads; of these: 8942268 (100.00%) were unpaired; of these: 1027964 (11.50%) aligned 0 times 7215089 (80.69%) aligned exactly 1 time 699215 (7.82%) aligned >1 times 88.50% overall alignment rate Time searching: 00:01:44 Overall time: 00:01:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1581821 / 7914304 = 0.1999 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:29:45: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:29:45: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:29:54: 1000000 INFO @ Sat, 24 Aug 2019 19:30:02: 2000000 INFO @ Sat, 24 Aug 2019 19:30:10: 3000000 INFO @ Sat, 24 Aug 2019 19:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:15: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:15: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:17: 4000000 INFO @ Sat, 24 Aug 2019 19:30:24: 1000000 INFO @ Sat, 24 Aug 2019 19:30:25: 5000000 INFO @ Sat, 24 Aug 2019 19:30:33: 6000000 INFO @ Sat, 24 Aug 2019 19:30:34: 2000000 INFO @ Sat, 24 Aug 2019 19:30:35: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:30:35: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:30:35: #1 total tags in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:30:35: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:30:36: #1 tags after filtering in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:30:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:30:36: #1 finished! INFO @ Sat, 24 Aug 2019 19:30:36: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:30:36: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:30:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:30:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:30:43: 3000000 INFO @ Sat, 24 Aug 2019 19:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:45: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:45: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:53: 4000000 INFO @ Sat, 24 Aug 2019 19:30:54: 1000000 INFO @ Sat, 24 Aug 2019 19:31:02: 5000000 INFO @ Sat, 24 Aug 2019 19:31:02: 2000000 INFO @ Sat, 24 Aug 2019 19:31:10: 3000000 INFO @ Sat, 24 Aug 2019 19:31:11: 6000000 INFO @ Sat, 24 Aug 2019 19:31:14: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:31:14: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:31:14: #1 total tags in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:31:14: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:14: #1 tags after filtering in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:31:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:14: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:14: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:15: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:31:18: 4000000 INFO @ Sat, 24 Aug 2019 19:31:26: 5000000 INFO @ Sat, 24 Aug 2019 19:31:34: 6000000 INFO @ Sat, 24 Aug 2019 19:31:36: #1 tag size is determined as 65 bps INFO @ Sat, 24 Aug 2019 19:31:36: #1 tag size = 65 INFO @ Sat, 24 Aug 2019 19:31:36: #1 total tags in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:31:36: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:36: #1 tags after filtering in treatment: 6332483 INFO @ Sat, 24 Aug 2019 19:31:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:36: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:36: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421885/SRX3421885.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。