Job ID = 2640831 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,389,377 reads read : 10,389,377 reads written : 10,389,377 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322058.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 10389377 reads; of these: 10389377 (100.00%) were unpaired; of these: 552656 (5.32%) aligned 0 times 8592173 (82.70%) aligned exactly 1 time 1244548 (11.98%) aligned >1 times 94.68% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2318508 / 9836721 = 0.2357 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:30:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:16: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:16: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:26: 1000000 INFO @ Sat, 24 Aug 2019 19:30:37: 2000000 INFO @ Sat, 24 Aug 2019 19:30:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:30:46: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:30:46: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:30:47: 3000000 INFO @ Sat, 24 Aug 2019 19:30:55: 1000000 INFO @ Sat, 24 Aug 2019 19:30:58: 4000000 INFO @ Sat, 24 Aug 2019 19:31:04: 2000000 INFO @ Sat, 24 Aug 2019 19:31:09: 5000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:31:14: 3000000 INFO @ Sat, 24 Aug 2019 19:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:31:16: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:31:16: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:31:21: 6000000 INFO @ Sat, 24 Aug 2019 19:31:23: 4000000 INFO @ Sat, 24 Aug 2019 19:31:28: 1000000 INFO @ Sat, 24 Aug 2019 19:31:31: 7000000 INFO @ Sat, 24 Aug 2019 19:31:32: 5000000 INFO @ Sat, 24 Aug 2019 19:31:37: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:31:37: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:31:37: #1 total tags in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:31:37: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:37: #1 tags after filtering in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:31:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:37: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:37: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:37: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:31:39: 2000000 INFO @ Sat, 24 Aug 2019 19:31:40: 6000000 INFO @ Sat, 24 Aug 2019 19:31:49: 7000000 INFO @ Sat, 24 Aug 2019 19:31:51: 3000000 INFO @ Sat, 24 Aug 2019 19:31:53: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:31:53: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:31:53: #1 total tags in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:31:53: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:31:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:31:53: #1 tags after filtering in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:31:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:31:53: #1 finished! INFO @ Sat, 24 Aug 2019 19:31:53: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:31:54: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:31:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:31:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:32:01: 4000000 INFO @ Sat, 24 Aug 2019 19:32:12: 5000000 INFO @ Sat, 24 Aug 2019 19:32:24: 6000000 INFO @ Sat, 24 Aug 2019 19:32:35: 7000000 INFO @ Sat, 24 Aug 2019 19:32:41: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:32:41: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:32:41: #1 total tags in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:32:41: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:32:41: #1 tags after filtering in treatment: 7518213 INFO @ Sat, 24 Aug 2019 19:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:32:41: #1 finished! INFO @ Sat, 24 Aug 2019 19:32:41: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:32:42: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:32:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:32:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421881/SRX3421881.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。