Job ID = 2640822 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,764,522 reads read : 9,764,522 reads written : 9,764,522 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322052.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:52 9764522 reads; of these: 9764522 (100.00%) were unpaired; of these: 159702 (1.64%) aligned 0 times 8780832 (89.93%) aligned exactly 1 time 823988 (8.44%) aligned >1 times 98.36% overall alignment rate Time searching: 00:01:52 Overall time: 00:01:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2407400 / 9604820 = 0.2506 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:24:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:24:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:24:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:24:42: 1000000 INFO @ Sat, 24 Aug 2019 19:24:49: 2000000 INFO @ Sat, 24 Aug 2019 19:24:57: 3000000 INFO @ Sat, 24 Aug 2019 19:25:04: 4000000 INFO @ Sat, 24 Aug 2019 19:25:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:25:05: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:25:05: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:25:12: 1000000 INFO @ Sat, 24 Aug 2019 19:25:12: 5000000 INFO @ Sat, 24 Aug 2019 19:25:19: 2000000 INFO @ Sat, 24 Aug 2019 19:25:20: 6000000 INFO @ Sat, 24 Aug 2019 19:25:26: 3000000 INFO @ Sat, 24 Aug 2019 19:25:28: 7000000 INFO @ Sat, 24 Aug 2019 19:25:29: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:25:29: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:25:29: #1 total tags in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:25:29: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:25:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:25:30: #1 tags after filtering in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:25:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:25:30: #1 finished! INFO @ Sat, 24 Aug 2019 19:25:30: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:25:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:25:30: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:25:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:25:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:25:34: 4000000 INFO @ Sat, 24 Aug 2019 19:25:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:25:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:25:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:25:42: 5000000 INFO @ Sat, 24 Aug 2019 19:25:42: 1000000 INFO @ Sat, 24 Aug 2019 19:25:49: 2000000 INFO @ Sat, 24 Aug 2019 19:25:50: 6000000 INFO @ Sat, 24 Aug 2019 19:25:57: 3000000 INFO @ Sat, 24 Aug 2019 19:25:58: 7000000 INFO @ Sat, 24 Aug 2019 19:26:00: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:26:00: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:26:00: #1 total tags in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:26:00: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:26:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:26:00: #1 tags after filtering in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:26:00: #1 finished! INFO @ Sat, 24 Aug 2019 19:26:00: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:26:01: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:26:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:26:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:26:05: 4000000 INFO @ Sat, 24 Aug 2019 19:26:13: 5000000 INFO @ Sat, 24 Aug 2019 19:26:21: 6000000 INFO @ Sat, 24 Aug 2019 19:26:29: 7000000 INFO @ Sat, 24 Aug 2019 19:26:30: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:26:30: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:26:30: #1 total tags in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:26:30: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:26:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:26:30: #1 tags after filtering in treatment: 7197420 INFO @ Sat, 24 Aug 2019 19:26:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:26:30: #1 finished! INFO @ Sat, 24 Aug 2019 19:26:30: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:26:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:26:31: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:26:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:26:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421875/SRX3421875.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。