Job ID = 2640820 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,332,086 reads read : 8,332,086 reads written : 8,332,086 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6322050.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:35 8332086 reads; of these: 8332086 (100.00%) were unpaired; of these: 431631 (5.18%) aligned 0 times 6888554 (82.68%) aligned exactly 1 time 1011901 (12.14%) aligned >1 times 94.82% overall alignment rate Time searching: 00:01:35 Overall time: 00:01:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1624893 / 7900455 = 0.2057 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:23:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:23:21: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:23:21: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:23:30: 1000000 INFO @ Sat, 24 Aug 2019 19:23:40: 2000000 INFO @ Sat, 24 Aug 2019 19:23:48: 3000000 INFO @ Sat, 24 Aug 2019 19:23:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:23:51: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:23:51: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:23:57: 4000000 INFO @ Sat, 24 Aug 2019 19:24:00: 1000000 INFO @ Sat, 24 Aug 2019 19:24:07: 5000000 INFO @ Sat, 24 Aug 2019 19:24:11: 2000000 INFO @ Sat, 24 Aug 2019 19:24:16: 6000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:24:18: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:24:18: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:24:18: #1 total tags in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:24:18: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:24:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:24:18: #1 tags after filtering in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:24:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:24:18: #1 finished! INFO @ Sat, 24 Aug 2019 19:24:18: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:24:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:24:19: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:24:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:24:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:24:20: 3000000 INFO @ Sat, 24 Aug 2019 19:24:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:24:21: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:24:21: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:24:29: 4000000 INFO @ Sat, 24 Aug 2019 19:24:30: 1000000 INFO @ Sat, 24 Aug 2019 19:24:37: 5000000 INFO @ Sat, 24 Aug 2019 19:24:39: 2000000 INFO @ Sat, 24 Aug 2019 19:24:46: 6000000 INFO @ Sat, 24 Aug 2019 19:24:48: 3000000 INFO @ Sat, 24 Aug 2019 19:24:48: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:24:48: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:24:48: #1 total tags in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:24:48: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:24:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:24:49: #1 tags after filtering in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:24:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:24:49: #1 finished! INFO @ Sat, 24 Aug 2019 19:24:49: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:24:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:24:49: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:24:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:24:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:24:57: 4000000 INFO @ Sat, 24 Aug 2019 19:25:05: 5000000 INFO @ Sat, 24 Aug 2019 19:25:14: 6000000 INFO @ Sat, 24 Aug 2019 19:25:16: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:25:16: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:25:16: #1 total tags in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:25:16: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:25:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:25:16: #1 tags after filtering in treatment: 6275562 INFO @ Sat, 24 Aug 2019 19:25:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:25:16: #1 finished! INFO @ Sat, 24 Aug 2019 19:25:16: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:25:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:25:17: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:25:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:25:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3421873/SRX3421873.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。