Job ID = 2010314 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,420,119 reads read : 23,420,119 reads written : 23,420,119 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:48 23420119 reads; of these: 23420119 (100.00%) were unpaired; of these: 942722 (4.03%) aligned 0 times 20046688 (85.60%) aligned exactly 1 time 2430709 (10.38%) aligned >1 times 95.97% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11135994 / 22477397 = 0.4954 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:25:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:25:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:25:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:25:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:25:40: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:25:40: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:25:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:25:41: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:25:41: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:25:51: 1000000 INFO @ Fri, 05 Jul 2019 22:25:52: 1000000 INFO @ Fri, 05 Jul 2019 22:25:53: 1000000 INFO @ Fri, 05 Jul 2019 22:26:02: 2000000 INFO @ Fri, 05 Jul 2019 22:26:04: 2000000 INFO @ Fri, 05 Jul 2019 22:26:05: 2000000 INFO @ Fri, 05 Jul 2019 22:26:13: 3000000 INFO @ Fri, 05 Jul 2019 22:26:15: 3000000 INFO @ Fri, 05 Jul 2019 22:26:16: 3000000 INFO @ Fri, 05 Jul 2019 22:26:23: 4000000 INFO @ Fri, 05 Jul 2019 22:26:26: 4000000 INFO @ Fri, 05 Jul 2019 22:26:27: 4000000 INFO @ Fri, 05 Jul 2019 22:26:34: 5000000 INFO @ Fri, 05 Jul 2019 22:26:37: 5000000 INFO @ Fri, 05 Jul 2019 22:26:38: 5000000 INFO @ Fri, 05 Jul 2019 22:26:44: 6000000 INFO @ Fri, 05 Jul 2019 22:26:47: 6000000 INFO @ Fri, 05 Jul 2019 22:26:48: 6000000 INFO @ Fri, 05 Jul 2019 22:26:55: 7000000 INFO @ Fri, 05 Jul 2019 22:26:58: 7000000 INFO @ Fri, 05 Jul 2019 22:26:59: 7000000 INFO @ Fri, 05 Jul 2019 22:27:06: 8000000 INFO @ Fri, 05 Jul 2019 22:27:09: 8000000 INFO @ Fri, 05 Jul 2019 22:27:09: 8000000 INFO @ Fri, 05 Jul 2019 22:27:16: 9000000 INFO @ Fri, 05 Jul 2019 22:27:18: 9000000 INFO @ Fri, 05 Jul 2019 22:27:19: 9000000 INFO @ Fri, 05 Jul 2019 22:27:26: 10000000 INFO @ Fri, 05 Jul 2019 22:27:27: 10000000 INFO @ Fri, 05 Jul 2019 22:27:29: 10000000 INFO @ Fri, 05 Jul 2019 22:27:33: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 22:27:36: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:27:36: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:27:36: #1 total tags in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:27:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:27:36: #1 tags after filtering in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:27:36: #1 finished! INFO @ Fri, 05 Jul 2019 22:27:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:27:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:27:37: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:27:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:27:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:27:37: 11000000 INFO @ Fri, 05 Jul 2019 22:27:39: 11000000 INFO @ Fri, 05 Jul 2019 22:27:41: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:27:41: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:27:41: #1 total tags in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:41: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:27:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:27:41: #1 tags after filtering in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:27:41: #1 finished! INFO @ Fri, 05 Jul 2019 22:27:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:27:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:27:42: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:27:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:27:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:27:43: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:27:43: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:27:43: #1 total tags in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:27:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:27:43: #1 tags after filtering in treatment: 11341403 INFO @ Fri, 05 Jul 2019 22:27:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:27:43: #1 finished! INFO @ Fri, 05 Jul 2019 22:27:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:27:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:27:44: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:27:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:27:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX335654/SRX335654.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。