Job ID = 10254450 sra ファイルのダウンロード中... Completed: 207364K bytes transferred in 9 seconds (178234K bits/sec), in 1 file. Completed: 234935K bytes transferred in 8 seconds (237640K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 8212758 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3353388/SRR6246250.sra Written 8212758 spots total Written 9272493 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3353388/SRR6246251.sra Written 9272493 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:33 17485251 reads; of these: 17485251 (100.00%) were unpaired; of these: 5318356 (30.42%) aligned 0 times 11416537 (65.29%) aligned exactly 1 time 750358 (4.29%) aligned >1 times 69.58% overall alignment rate Time searching: 00:02:33 Overall time: 00:02:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4747116 / 12166895 = 0.3902 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 06 Dec 2017 18:18:46: # Command line: callpeak -t SRX3353388.bam -f BAM -g 12100000 -n SRX3353388.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3353388.20 # format = BAM # ChIP-seq file = ['SRX3353388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 06 Dec 2017 18:18:46: #1 read tag files... INFO @ Wed, 06 Dec 2017 18:18:46: #1 read treatment tags... INFO @ Wed, 06 Dec 2017 18:18:46: # Command line: callpeak -t SRX3353388.bam -f BAM -g 12100000 -n SRX3353388.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3353388.05 # format = BAM # ChIP-seq file = ['SRX3353388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 06 Dec 2017 18:18:46: #1 read tag files... INFO @ Wed, 06 Dec 2017 18:18:46: #1 read treatment tags... INFO @ Wed, 06 Dec 2017 18:18:46: # Command line: callpeak -t SRX3353388.bam -f BAM -g 12100000 -n SRX3353388.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3353388.10 # format = BAM # ChIP-seq file = ['SRX3353388.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 06 Dec 2017 18:18:46: #1 read tag files... INFO @ Wed, 06 Dec 2017 18:18:46: #1 read treatment tags... INFO @ Wed, 06 Dec 2017 18:18:53: 1000000 INFO @ Wed, 06 Dec 2017 18:18:53: 1000000 INFO @ Wed, 06 Dec 2017 18:18:53: 1000000 INFO @ Wed, 06 Dec 2017 18:19:00: 2000000 INFO @ Wed, 06 Dec 2017 18:19:00: 2000000 INFO @ Wed, 06 Dec 2017 18:19:00: 2000000 INFO @ Wed, 06 Dec 2017 18:19:06: 3000000 INFO @ Wed, 06 Dec 2017 18:19:06: 3000000 INFO @ Wed, 06 Dec 2017 18:19:07: 3000000 INFO @ Wed, 06 Dec 2017 18:19:13: 4000000 INFO @ Wed, 06 Dec 2017 18:19:13: 4000000 INFO @ Wed, 06 Dec 2017 18:19:14: 4000000 INFO @ Wed, 06 Dec 2017 18:19:20: 5000000 INFO @ Wed, 06 Dec 2017 18:19:20: 5000000 INFO @ Wed, 06 Dec 2017 18:19:20: 5000000 INFO @ Wed, 06 Dec 2017 18:19:26: 6000000 INFO @ Wed, 06 Dec 2017 18:19:27: 6000000 INFO @ Wed, 06 Dec 2017 18:19:27: 6000000 INFO @ Wed, 06 Dec 2017 18:19:33: 7000000 INFO @ Wed, 06 Dec 2017 18:19:33: 7000000 INFO @ Wed, 06 Dec 2017 18:19:34: 7000000 INFO @ Wed, 06 Dec 2017 18:19:36: #1 tag size is determined as 51 bps INFO @ Wed, 06 Dec 2017 18:19:36: #1 tag size = 51 INFO @ Wed, 06 Dec 2017 18:19:36: #1 total tags in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:36: #1 user defined the maximum tags... INFO @ Wed, 06 Dec 2017 18:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 06 Dec 2017 18:19:36: #1 tags after filtering in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 06 Dec 2017 18:19:36: #1 finished! INFO @ Wed, 06 Dec 2017 18:19:36: #2 Build Peak Model... INFO @ Wed, 06 Dec 2017 18:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 06 Dec 2017 18:19:36: #1 tag size is determined as 51 bps INFO @ Wed, 06 Dec 2017 18:19:36: #1 tag size = 51 INFO @ Wed, 06 Dec 2017 18:19:36: #1 total tags in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:36: #1 user defined the maximum tags... INFO @ Wed, 06 Dec 2017 18:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 06 Dec 2017 18:19:36: #1 tags after filtering in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 06 Dec 2017 18:19:36: #1 finished! INFO @ Wed, 06 Dec 2017 18:19:36: #2 Build Peak Model... INFO @ Wed, 06 Dec 2017 18:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 06 Dec 2017 18:19:36: #2 number of paired peaks: 0 WARNING @ Wed, 06 Dec 2017 18:19:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 06 Dec 2017 18:19:36: Process for pairing-model is terminated! cat: SRX3353388.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3353388.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 06 Dec 2017 18:19:37: #2 number of paired peaks: 0 WARNING @ Wed, 06 Dec 2017 18:19:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 06 Dec 2017 18:19:37: Process for pairing-model is terminated! cat: SRX3353388.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3353388.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 06 Dec 2017 18:19:37: #1 tag size is determined as 51 bps INFO @ Wed, 06 Dec 2017 18:19:37: #1 tag size = 51 INFO @ Wed, 06 Dec 2017 18:19:37: #1 total tags in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:37: #1 user defined the maximum tags... INFO @ Wed, 06 Dec 2017 18:19:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 06 Dec 2017 18:19:37: #1 tags after filtering in treatment: 7419779 INFO @ Wed, 06 Dec 2017 18:19:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 06 Dec 2017 18:19:37: #1 finished! INFO @ Wed, 06 Dec 2017 18:19:37: #2 Build Peak Model... INFO @ Wed, 06 Dec 2017 18:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 06 Dec 2017 18:19:37: #2 number of paired peaks: 0 WARNING @ Wed, 06 Dec 2017 18:19:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 06 Dec 2017 18:19:37: Process for pairing-model is terminated! cat: SRX3353388.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3353388.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3353388.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。